Title:
NUCLEIC ACID PROBES AND BROAD-RANGE PRIMERS FROM REGIONS IN DNA DIRECTED RNA POLYMERASE SUBUNIT B GENES, AND METHODS IN WHICH THEY ARE USED
Document Type and Number:
WIPO Patent Application WO2005059156
Kind Code:
A3
Abstract:
The invention relates to nucleic acid probes and to broad-range primers that are useful in the identification of bacte-rial species and the diagnosis of bacterial infections. Es-pecially, the invention relates to specific nucleic acid probes that originate from hyper-variable regions situated near the conserved sequences of the gene for RNA poly-merase beta subunit, rpoB (DNA directed RNA poly-merase subunit B) of infection-causing bacteria. The in-vention also relates to broad-range primers originating from the conserved regions of rpoB genes. In addition, the invention relates to the use of these nucleic acid probes and broad-range primers in the diagnosis of bacterial in-fections as well as to diagnostic methods in which these nucleic acid probes and broad-range primers are used.
Inventors:
PALGI JAAN (FI)
JALAVA JARI (FI)
NIKKARI SIMO (FI)
JALAVA JARI (FI)
NIKKARI SIMO (FI)
Application Number:
PCT/FI2004/000776
Publication Date:
September 09, 2005
Filing Date:
December 17, 2004
Export Citation:
Assignee:
MOBIDIAG OY (FI)
PALGI JAAN (FI)
JALAVA JARI (FI)
NIKKARI SIMO (FI)
PALGI JAAN (FI)
JALAVA JARI (FI)
NIKKARI SIMO (FI)
International Classes:
C12N15/11; C12Q1/68; C12N; C12Q; (IPC1-7): C12Q1/68; C12N15/11
Domestic Patent References:
| WO2001092573A1 | 2001-12-06 | |||
| WO2001031061A1 | 2001-05-03 | |||
| WO1995033851A2 | 1995-12-14 | |||
| WO2003008645A1 | 2003-01-30 | |||
| WO2003020972A1 | 2003-03-13 | |||
| WO2003016534A1 | 2003-02-27 | |||
| WO2004041841A2 | 2004-05-21 |
Foreign References:
| US5786147A | 1998-07-28 |
Other References:
KHAMIS A. ET AL: "Usefulness of rpoB Gene Sequencing for Identification of Afipia and Bosea Species, Including a Strategy for Chossing Discriminative Partial Sequences.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 69, no. 11, November 2003 (2003-11-01), pages 6740 - 6749, XP002999723
DAHLLOF I ET AL: "rpoB-Based Microbial Community Analysis Avoids Limitations Inherent in 16S rRNA Gene Intraspecies Heterogeneity.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 66, no. 8, August 2000 (2000-08-01), pages 3376 - 3380, XP002999724
MOLLET C. ET AL: "rpoB sequence analysis as a novel basis for bacterial identification.", MOLECULAR MICROBIOLOGY., vol. 26, no. 5, 1997, pages 1005 - 1011, XP000913977
DAHLLOF I ET AL: "rpoB-Based Microbial Community Analysis Avoids Limitations Inherent in 16S rRNA Gene Intraspecies Heterogeneity.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 66, no. 8, August 2000 (2000-08-01), pages 3376 - 3380, XP002999724
MOLLET C. ET AL: "rpoB sequence analysis as a novel basis for bacterial identification.", MOLECULAR MICROBIOLOGY., vol. 26, no. 5, 1997, pages 1005 - 1011, XP000913977
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