Title:
PEPTIDE ANALYSIS USING A SOLID SUPPORT
Document Type and Number:
WIPO Patent Application WO2002095419
Kind Code:
A3
Abstract:
The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.
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Inventors:
BHIKHABHAI RAMAGAURI (SE)
LIMINGA MARIA (SE)
MALOISEL JEAN-LUC (SE)
PALMGREN RONNIE (SE)
KEOUGH THOMAS W (US)
YOUNGQUIST ROBERT S (US)
VAUGHN HAROLD L (US)
YELM KEN E (US)
LIMINGA MARIA (SE)
MALOISEL JEAN-LUC (SE)
PALMGREN RONNIE (SE)
KEOUGH THOMAS W (US)
YOUNGQUIST ROBERT S (US)
VAUGHN HAROLD L (US)
YELM KEN E (US)
Application Number:
PCT/US2002/016247
Publication Date:
April 24, 2003
Filing Date:
May 20, 2002
Export Citation:
Assignee:
AMERSHAM BIOSCIENCES AB (SE)
PROCTER & GAMBLE (US)
BHIKHABHAI RAMAGAURI (SE)
LIMINGA MARIA (SE)
MALOISEL JEAN-LUC (SE)
PALMGREN RONNIE (SE)
KEOUGH THOMAS W (US)
YOUNGQUIST ROBERT S (US)
VAUGHN HAROLD L (US)
YELM KEN E (US)
PROCTER & GAMBLE (US)
BHIKHABHAI RAMAGAURI (SE)
LIMINGA MARIA (SE)
MALOISEL JEAN-LUC (SE)
PALMGREN RONNIE (SE)
KEOUGH THOMAS W (US)
YOUNGQUIST ROBERT S (US)
VAUGHN HAROLD L (US)
YELM KEN E (US)
International Classes:
G01N27/62; G01N33/58; G01N33/68; (IPC1-7): G01N33/58; C12Q1/37; G01N33/68
Domestic Patent References:
| WO2000043792A2 | 2000-07-27 |
Foreign References:
| US3478013A | 1969-11-11 | |||
| US4296109A | 1981-10-20 |
Other References:
KEOUGH ET AL: "A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionization mass spectrometry", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, June 1999 (1999-06-01), pages 7131 - 7136, XP002141709, ISSN: 0027-8424
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1980, FINK G ET AL: "REAGENTS SUITABLE FOR THE CROSS LINKING OF NUCLEIC-ACIDS TO PROTEINS", XP002227343, Database accession no. PREV198171050036
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1983, KEANA J F W ET AL: "DETERGENTS CONTAINING A 1 3 DIENE GROUP IN THE HYDROPHOBIC SEGMENT FACILE CHEMICAL MODIFICATION BY A DIELS ALDER REACTION WITH HYDROPHILIC DIENOPHILES IN AQUEOUS SOLUTION", XP002227344, Database accession no. PREV198477032524
KEOUGH T ET AL: "Solid-phase derivatization of tryptic peptides for rapid protein identification by matrix-assisted laser desorption/ionization mass spectrometry.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 16, no. 11, 2002, pages 1003 - 1015, XP009004084, ISSN: 0951-4198
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1980, FINK G ET AL: "REAGENTS SUITABLE FOR THE CROSS LINKING OF NUCLEIC-ACIDS TO PROTEINS", XP002227343, Database accession no. PREV198171050036
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1983, KEANA J F W ET AL: "DETERGENTS CONTAINING A 1 3 DIENE GROUP IN THE HYDROPHOBIC SEGMENT FACILE CHEMICAL MODIFICATION BY A DIELS ALDER REACTION WITH HYDROPHILIC DIENOPHILES IN AQUEOUS SOLUTION", XP002227344, Database accession no. PREV198477032524
KEOUGH T ET AL: "Solid-phase derivatization of tryptic peptides for rapid protein identification by matrix-assisted laser desorption/ionization mass spectrometry.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 16, no. 11, 2002, pages 1003 - 1015, XP009004084, ISSN: 0951-4198
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