Title:
POLYCISTRONIC EXPRESSION OF ANTIBODIES
Document Type and Number:
WIPO Patent Application WO2003048306
Kind Code:
A3
Abstract:
Described herein is a novel expression system for producing multiple gene products of interest from a single polycistronic construct. In particular, the expression system contains a polycistronic vector capable of expressing functional antibodies in eukaryotic host cells, which vector contains at least the following elements operably linked in the 5' to 3' orientation: a promoter operable in a eukaryotic cell; a DNA sequence encoding at least the variable region of an antibody light chain; an internal ribosome entry site (IRES); and at least one DNA sequence encoding an antibody heavy chain. Also disclosed are mammalian cells containing the polycistronic expression vector, and a method of producing functional antibodies in mammalian cells transfected with the polycistronic expression vector.
Inventors:
REFF MITCHELL (US)
BARNETT RICHARD (US)
BARNETT RICHARD (US)
Application Number:
PCT/US2002/036856
Publication Date:
December 11, 2003
Filing Date:
November 18, 2002
Export Citation:
Assignee:
IDEC PHARMA CORP (US)
REFF MITCHELL (US)
BARNETT RICHARD (US)
REFF MITCHELL (US)
BARNETT RICHARD (US)
International Classes:
A61K47/48; A61K51/10; A61P35/00; C07K16/00; C07K16/28; C07K16/30; C12N15/09; C12N1/19; C12N5/10; C12N15/12; C12N15/85; C12P21/02; (IPC1-7): C12N15/63; C12N15/12; C12N15/85; C12N15/86; C12N15/00; C07K16/00
Domestic Patent References:
| WO1993011161A1 | 1993-06-10 |
Foreign References:
| US6060273A | 2000-05-09 | |||
| US5843685A | 1998-12-01 | |||
| US5693762A | 1997-12-02 | |||
| US5843439A | 1998-12-01 |
Other References:
"Invitrogen Catalog", 1997, pages 14 - 19, XP002967953
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