Title:
PRIMITIVE GUT ENDODERM CELLS AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2016/093222
Kind Code:
A1
Abstract:
Provided is a fundamental technology for inexpensively and safely inducing a large amount of human organ cells. Provided are cells that are induced after differentiating pluripotent stem cells and then subculturing the cells at least once. Said cells: comprise the undifferentiated (pluripotent) cell markers NANOG, OCT4, MYC and LIN28A, which are negative, the endoderm cell markers CXCR4, CER1, HHEX and GATA4, which are negative, the gut endoderm cell markers CDX2 and HOXB9, which are positive, the mesenchymal cell marker brachyury (T), which is negative, and the pancreatic cell marker PDX1, which is negative; and can be differentiated into at least liver cells, pancreatic cells and intestinal cells. Further provided are methods for producing and amplifying the cells, a method for producing organ cells using the abovementioned cells, and a method for creating a working cell bank for freezing and storing the abovementioned cells and producing organ cells.
Inventors:
TAKEBE TAKANORI (JP)
TANIGUCHI HIDEKI (JP)
ZHANG RAN RAN (JP)
TANIGUCHI HIDEKI (JP)
ZHANG RAN RAN (JP)
Application Number:
PCT/JP2015/084379
Publication Date:
June 16, 2016
Filing Date:
December 08, 2015
Export Citation:
Assignee:
PUBLIC UNIV CORP YOKOHAMA CITY (JP)
International Classes:
C12N5/074; C12N5/10
Foreign References:
| US20140329321A1 | 2014-11-06 |
Other References:
WANG, PEI ET AL.: "Targeting SOX17 in Human Embryonic Stem Cells Creates Unique Strategies for Isolating and Analyzing Developing Endoderm", CELL STEM CELL, vol. 8, no. 3, 4 March 2011 (2011-03-04), pages 335 - 346, XP028183971, ISSN: 1934-5909, DOI: doi:10.1016/j.stem.2011.01.017
TAKEBE, T ET AL.: "Human iPSC-Derived Miniature Organs: A Tool for Drug Studies", CLIN PHARMACOL THER, vol. 96, no. 3, September 2014 (2014-09-01), pages 310 - 313, XP055173771, ISSN: 1532-6535, DOI: doi:10.1038/clpt.2014.110
HASSANI, S.-N. ET AL.: "Inhibition of TGFβ Signaling Promotes Ground State Pluripotency", STEM CELL REV AND REP, vol. 10, no. 1, February 2014 (2014-02-01), pages 16 - 30, ISSN: 1550-8943
See also references of EP 3239294A4
TAKEBE, T ET AL.: "Human iPSC-Derived Miniature Organs: A Tool for Drug Studies", CLIN PHARMACOL THER, vol. 96, no. 3, September 2014 (2014-09-01), pages 310 - 313, XP055173771, ISSN: 1532-6535, DOI: doi:10.1038/clpt.2014.110
HASSANI, S.-N. ET AL.: "Inhibition of TGFβ Signaling Promotes Ground State Pluripotency", STEM CELL REV AND REP, vol. 10, no. 1, February 2014 (2014-02-01), pages 16 - 30, ISSN: 1550-8943
See also references of EP 3239294A4
Attorney, Agent or Firm:
MAYAMA, Setsuko et al. (JP)
Setsuko Mayama (JP)
Setsuko Mayama (JP)
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