Title:
PROCESS FOR EXTRACTING SULFURIC ACID CONJUGATE AND GLUCURONIC ACID CONJUGATE OF STEROID
Document Type and Number:
WIPO Patent Application WO/1989/012825
Kind Code:
A1
Abstract:
A process for extracting sulfuric acid conjugate and glucuronic acid conjugate of steroid, which comprises converting a sulfuric acid conjugate of steroid and a glucuronic acid conjugate of steroid in a sample solution (e.g., urine) to tetrapentylammonium ion pairs, first extracting the ion pair of the sulfuric acid conjugate with benzene or the like at a pH of 6 or more utilizing difference in extractability between the two ion pairs with organic solvents, adding sodium sulfate to the remaining sample solution in an amount of 20 wt % or more and, after dissolving the sodium sulfate, extracting the ion pair of the glucuronic acid conjugate with dichloromethane or the like at a pH of 7 or more. Also disclosed is another process for extracting the two ion pairs with dichloromethane or the like, which comprises adding 20 wt % or more sodium sulfate and extracting the ion pairs between the two conjugates and a tetraalkylammonium ion wherein the straight chain of the alkyl group contains 5 to 7 carbon atoms with dichloromethane or the like at a pH of 7 or more utilizing common extractability of the two ion pairs with organic solvents.
Inventors:
IWATA JIHEI (JP)
Application Number:
PCT/JP1988/000567
Publication Date:
December 28, 1989
Filing Date:
June 13, 1988
Export Citation:
Assignee:
IWATA JIHEI (JP)
International Classes:
G01N33/50; G01N33/74; (IPC1-7): G01N33/74; G01N33/493
Foreign References:
| JPS59155761A | 1984-09-04 | |||
| JPS53103798A | 1978-09-09 | |||
| JPS5155294A | 1976-05-14 |
Other References:
JOURNAL OF STEROID BIOCHEMISTRY, 1976, Vol. 7, pp. 615-629.
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