Title:
PROCESS FOR THE FERMENTATIVE PREPARATION OF L-AMINO ACIDS USING CORYNEFORM BACTERIA
Document Type and Number:
WIPO Patent Application WO2003014370
Kind Code:
A3
Abstract:
The invention relates to a process for the preparation of L-amino acids, in which the following steps are carried out: a) fermentation of the coryneform bacteria which produce the desired L-amino acid and in which at least the gene which codes for trehalose phosphatase and/or the gene which codes for maltooligosyl-trehalose synthase and/or the gene which codes for maltooligosyl-trehalose trehalohydrolase is or are attenuated, b) concentration of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the L-amino acid,and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are addtionally enhanced are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed.
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Inventors:
WOLF ANDREAS
SCHISCHKA NATALIE
HERMANN THOMAS
MORBACH SUSANNE
KRAEMER REINHARD
SCHISCHKA NATALIE
HERMANN THOMAS
MORBACH SUSANNE
KRAEMER REINHARD
Application Number:
PCT/EP2002/005264
Publication Date:
December 11, 2003
Filing Date:
May 14, 2002
Export Citation:
Assignee:
DEGUSSA (DE)
International Classes:
C12N1/21; C12N9/10; C12N9/90; C12P13/08; C12P13/14; (IPC1-7): C12N9/10; C12P13/04
Domestic Patent References:
| WO1997038111A1 | 1997-10-16 |
Foreign References:
| EP1174508A2 | 2002-01-23 |
Other References:
DATABASE GENBANK [online] 24 January 2001 (2001-01-24), POMPEJUS, M. ET AL.: "Corynebacterium glutamicum genes encoding metabolic pathway proteins", XP002231768, retrieved from NCBI Database accession no. AX064857
DATABASE GENBANK [online] 24 January 2001 (2001-01-24), POMPEJUS, M. ET AL.: "Corynebacterium glutamicum genes encoding metabolic pathway proteins", XP002231769, retrieved from NCBI Database accession no. Ax064861
DATABASE GENBANK [online] 24 January 2001 (2001-01-24), POMPEJUS, M. ET AL.: "Corynebacterium glutamicum genes encoding metabolic pathway proteins", XP002231770, retrieved from NCBI Database accession no. Ax064863
MARTIN J F ET AL: "CLONING SYSTEMS IN AMINO ACID-PRODUCING CORYNEBACTERIA", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 5, 1 February 1987 (1987-02-01), pages 137 - 146, XP002034056, ISSN: 0733-222X
DATABASE GENBANK [online] 24 January 2001 (2001-01-24), POMPEJUS, M. ET AL.: "Corynebacterium glutamicum genes encoding metabolic pathway proteins", XP002231769, retrieved from NCBI Database accession no. Ax064861
DATABASE GENBANK [online] 24 January 2001 (2001-01-24), POMPEJUS, M. ET AL.: "Corynebacterium glutamicum genes encoding metabolic pathway proteins", XP002231770, retrieved from NCBI Database accession no. Ax064863
MARTIN J F ET AL: "CLONING SYSTEMS IN AMINO ACID-PRODUCING CORYNEBACTERIA", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 5, 1 February 1987 (1987-02-01), pages 137 - 146, XP002034056, ISSN: 0733-222X
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