Title:
PROCESS FOR PRODUCING L-LYSINE AND L-ARGININE USING METHANOL ASSIMILATING BACTERIUM
Document Type and Number:
WIPO Patent Application WO/2006/059715
Kind Code:
A1
Abstract:
DNA is introduced into a methanol-assimilating bacterium such as a Methylobacillus bacterium and the obtained bacterium is used for producing L-lysine and L-arginine. The DNA has a nucleotide sequence encoding LysE protein derived from a coryneform bacterium, which has a loop region and six hydrophobic helixes, wherein a region encoding the loop region has been modified in such a manner that a stop codon is present in all three reading frames, and facilitates secretion of L-lysine or L-arginine or both of these L-amino acids to the outside of the cell of the methanol-assimilating bacterium when the DNA is introduced into the bacterium.
Inventors:
GUNJI YOSHIYA (JP)
ITO HISAO (JP)
ITO HISAO (JP)
Application Number:
PCT/JP2005/022180
Publication Date:
June 08, 2006
Filing Date:
December 02, 2005
Export Citation:
Assignee:
AJINOMOTO KK (JP)
GUNJI YOSHIYA (JP)
ITO HISAO (JP)
GUNJI YOSHIYA (JP)
ITO HISAO (JP)
International Classes:
C12N1/21; C12P13/08; C12P13/10
Foreign References:
| JP2004166594A | 2004-06-17 |
Other References:
FORMAN B M ET AL: "pEXPRESS: a family of expression vectors containing a single transcription unit active in prokaryotes, eukaryotes and in vitro.", GENE., vol. 105, no. 1, 1991, pages 9 - 15, XP002995237
PETTERSSON R F ET AL: "Chemical synthesis and molecular cloning of a STOP oligonucleotide encoding an UGA translation terminator in all three reading frames.", GENE., vol. 24, no. 1, 1983, pages 15 - 27, XP000905050
PETTERSSON R F ET AL: "Chemical synthesis and molecular cloning of a STOP oligonucleotide encoding an UGA translation terminator in all three reading frames.", GENE., vol. 24, no. 1, 1983, pages 15 - 27, XP000905050
Attorney, Agent or Firm:
Kawaguchi, Yoshiyuki (4-10 Higashi Nihonbashi 3-chom, Chuo-ku Tokyo 04, JP)
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