Title:
PRODUCTION OF ASCORBIC ACID IN PLANTS
Document Type and Number:
WIPO Patent Application WO1998050558
Kind Code:
A3
Abstract:
The present invention relates to a polynucleotide in isolated form, which polynucleotide codes for a protein with the activity of the enzyme L-galactono-gamma-lactone dehydrogenase, which polynucleotide comprises at least the L-galactono-gamma-lactone dehydrogenase activity-determining parts of the coding part of the sequence or a sequence derived therefrom on the basis of the degeneration of the genetic code. The invention further relates to the use of the polynucleotide in the production of transgenic plants, plant cells, or other eukaryotic cells.
Inventors:
BAUW GUY JEROME CORNEEL (BE)
DAVEY MARK WILLIAM (BE)
OSTERGAARD JENS (BE)
VAN MONTAGU MARC CHARLES ERNES (BE)
DAVEY MARK WILLIAM (BE)
OSTERGAARD JENS (BE)
VAN MONTAGU MARC CHARLES ERNES (BE)
Application Number:
PCT/EP1998/002830
Publication Date:
February 04, 1999
Filing Date:
May 07, 1998
Export Citation:
Assignee:
VLAAMS INTERUNIV INST BIOTECH (BE)
BAUW GUY JEROME CORNEEL (BE)
DAVEY MARK WILLIAM (BE)
OSTERGAARD JENS (BE)
MONTAGU MARC CHARLES ERNEST VA (BE)
BAUW GUY JEROME CORNEEL (BE)
DAVEY MARK WILLIAM (BE)
OSTERGAARD JENS (BE)
MONTAGU MARC CHARLES ERNEST VA (BE)
International Classes:
C12N9/02; C12N15/53; C12N15/82; C12P17/04; (IPC1-7): C12N15/53; C12N9/02; C12N15/82; A01H5/00
Domestic Patent References:
| WO1997004100A2 | 1997-02-06 | |||
| WO1985001745A1 | 1985-04-25 | |||
| WO1996031115A1 | 1996-10-10 | |||
| WO1996017944A2 | 1996-06-13 | |||
| WO1993019193A1 | 1993-09-30 |
Foreign References:
| US4259443A | 1981-03-31 |
Other References:
YAGI, ET AL.: "Expression in monkey cells of the missing enzyme in L-ascorbic acid biosynthesis, L-glucono-gamma-lactone oxidase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 177, no. 2, 1991, pages 659 - 663, XP002054716
MINOBE, Y., ET AL.: "Rice cDNA, partial sequence (R0642_1A)", EMBL DATABASE, REL.37 29-NOV-1993, ACCESSION NO. D23947, XP002054717
OSTERGAARD, J., ET AL.: "Isolation of a cDNA coding for L-galactono-gamma-lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 28 November 1997 (1997-11-28), pages 30009 - 30016, XP002054719
BAI Y ET AL: "L-GALACTONO-1,4-LACTONE: FERRICYTICHROME-C OXIDOREDUCTASE E.C.1.3.2.3 FROM PEAS AND ITS USE IN A BIOPROCESS FOR L-ASCORBIC ACID SYNTHESIS", BIOLOGICAL CHEMISTRY HOPPE-SEYLER, vol. 373, no. 9, 25 September 1992 (1992-09-25), pages 857/858, XP002025678
OBA, K., ET AL.: "Purification and properties of L-galactono-gamma-lactone dehydrogenase, a key entyme for ascorbic acid biosynthesis, from sweet potato roots.", J. BIOCHEM., vol. 117, 1995, pages 120 - 124, XP002054718
CHEMICAL ABSTRACTS, vol. 125, no. 15, 1996, Columbus, Ohio, US; abstract no. 190777, ISHII, G., ET AL.: "Biochemical approach to higher ascorbic acid levels in potato breeding" XP002080811
KULBE K D ET AL: "ENZYMATIC SYNTHESIS OF L-ASCORBIC ACID VIA D-URONIC ACIDS: MEMBRANE-REACTOR INTEGRATED RECOVERY OF D-GALACTURONIC ACID FROM PECTIN HYDROLYSATES", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 506, 1987, pages 543 - 551, XP000646778
CHEMICAL ABSTRACTS, vol. 124, no. 9, 1995, Columbus, Ohio, US; abstract no. 115786, FUKADA,M., ET AL.: "Effect of storage time of potatoes after harvest on increase in the ascorbic acid content by wounding" XP002054720
DATABASE WPI Section Ch Week 8920, Derwent World Patents Index; Class B04, AN 89-148142, XP002054721
MINOBE, Y., ET AL.: "Rice cDNA, partial sequence (R0642_1A)", EMBL DATABASE, REL.37 29-NOV-1993, ACCESSION NO. D23947, XP002054717
OSTERGAARD, J., ET AL.: "Isolation of a cDNA coding for L-galactono-gamma-lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 28 November 1997 (1997-11-28), pages 30009 - 30016, XP002054719
BAI Y ET AL: "L-GALACTONO-1,4-LACTONE: FERRICYTICHROME-C OXIDOREDUCTASE E.C.1.3.2.3 FROM PEAS AND ITS USE IN A BIOPROCESS FOR L-ASCORBIC ACID SYNTHESIS", BIOLOGICAL CHEMISTRY HOPPE-SEYLER, vol. 373, no. 9, 25 September 1992 (1992-09-25), pages 857/858, XP002025678
OBA, K., ET AL.: "Purification and properties of L-galactono-gamma-lactone dehydrogenase, a key entyme for ascorbic acid biosynthesis, from sweet potato roots.", J. BIOCHEM., vol. 117, 1995, pages 120 - 124, XP002054718
CHEMICAL ABSTRACTS, vol. 125, no. 15, 1996, Columbus, Ohio, US; abstract no. 190777, ISHII, G., ET AL.: "Biochemical approach to higher ascorbic acid levels in potato breeding" XP002080811
KULBE K D ET AL: "ENZYMATIC SYNTHESIS OF L-ASCORBIC ACID VIA D-URONIC ACIDS: MEMBRANE-REACTOR INTEGRATED RECOVERY OF D-GALACTURONIC ACID FROM PECTIN HYDROLYSATES", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 506, 1987, pages 543 - 551, XP000646778
CHEMICAL ABSTRACTS, vol. 124, no. 9, 1995, Columbus, Ohio, US; abstract no. 115786, FUKADA,M., ET AL.: "Effect of storage time of potatoes after harvest on increase in the ascorbic acid content by wounding" XP002054720
DATABASE WPI Section Ch Week 8920, Derwent World Patents Index; Class B04, AN 89-148142, XP002054721
Download PDF:
Previous Patent: INTRACELLULAR PRODUCTION OF HEPATITIS C E1 AND E2 TRUNCATED POLYPEPTIDES
Next Patent: HUMAN PROHORMONE CONVERTASE 4
Next Patent: HUMAN PROHORMONE CONVERTASE 4