Title:
REAGENT AND METHOD FOR THE DETECTION OF A NUCLEOTIDE SEQUENCE WITH SIGNAL AMPLIFICATION
Document Type and Number:
WIPO Patent Application WO1995008000
Kind Code:
A3
Abstract:
PCT No. PCT/FR94/01084 Sec. 371 Date Jun. 14, 1995 Sec. 102(e) Date Jun. 14, 1995 PCT Filed Sep. 15, 1994 PCT Pub. No. WO95/08000 PCT Pub. Date Mar. 23, 1995Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods comprise use of a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
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Inventors:
MANDRAND BERNARD (FR)
CROS PHILIPPE (FR)
DELAIR THIERRY (FR)
CHARLES MARIE-HELENE (FR)
EROUT MARIE-NOELLE (FR)
PICHOT CHRISTIAN (FR)
CROS PHILIPPE (FR)
DELAIR THIERRY (FR)
CHARLES MARIE-HELENE (FR)
EROUT MARIE-NOELLE (FR)
PICHOT CHRISTIAN (FR)
Application Number:
PCT/FR1994/001084
Publication Date:
June 08, 1995
Filing Date:
September 15, 1994
Export Citation:
Assignee:
BIO MERIEUX (FR)
MANDRAND BERNARD (FR)
CROS PHILIPPE (FR)
DELAIR THIERRY (FR)
CHARLES MARIE HELENE (FR)
EROUT MARIE NOELLE (FR)
PICHOT CHRISTIAN (FR)
MANDRAND BERNARD (FR)
CROS PHILIPPE (FR)
DELAIR THIERRY (FR)
CHARLES MARIE HELENE (FR)
EROUT MARIE NOELLE (FR)
PICHOT CHRISTIAN (FR)
International Classes:
C08G81/02; C12Q1/68; C12Q1/6813; C12Q1/682; (IPC1-7): C12Q1/68; C08G81/02
Domestic Patent References:
| WO1991008307A1 | 1991-06-13 |
Foreign References:
| US4925785A | 1990-05-15 | |||
| EP0450594A2 | 1991-10-09 | |||
| EP0317077A1 | 1989-05-14 |
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