Title:
RIBOZYMES CAPABLE OF CONFERRING RESISTANCE TO POTYVIRUS INFECTION, AND PLANTS EXPRESSING SAID RIBOZYMES
Document Type and Number:
WIPO Patent Application WO1999028485
Kind Code:
A3
Abstract:
The invention concerns a nucleic acid molecule, called polyribozyme, having endoribonuclease activity, comprising at least first and second hybridising regions and at least two catalytic regions, said first and second hybridizing regions being complementary to first and second target regions, respectively, in the genomic (+) RNA of a potyvirus, or in the replicative (-) strand RNA of a potyvirus, said first and second target regions being either: within or comprising different genes or untranslated regions on the same RNA strand, or on different RNA strands, or within a region corresponding to one gene or cistron on the replicative (-) strand RNA.
Inventors:
HUTTNER ERIC (FR)
TUCKER WILLIAM (AU)
VERMEULEN AGNES (FR)
IGNART FREDERIC (FR)
TUCKER WILLIAM (AU)
VERMEULEN AGNES (FR)
IGNART FREDERIC (FR)
Application Number:
PCT/EP1998/007871
Publication Date:
September 02, 1999
Filing Date:
December 03, 1998
Export Citation:
Assignee:
GENE SHEARS PTY LTD (AU)
HUTTNER ERIC (FR)
TUCKER WILLIAM (AU)
VERMEULEN AGNES (FR)
IGNART FREDERIC (FR)
HUTTNER ERIC (FR)
TUCKER WILLIAM (AU)
VERMEULEN AGNES (FR)
IGNART FREDERIC (FR)
International Classes:
C07H21/02; C12N5/10; C12N9/00; C12N9/22; A01H5/00; C12N15/09; C12N15/113; C12N15/82; C12Q1/68; C12R1/92; (IPC1-7): C12N15/82; C12N9/00; A01H5/00; C07H21/02
Domestic Patent References:
| WO1994019476A1 | 1994-09-01 | |||
| WO1995003404A1 | 1995-02-02 | |||
| WO1994000012A1 | 1994-01-06 | |||
| WO1993014210A1 | 1993-07-22 | |||
| WO1995004825A1 | 1995-02-16 | |||
| WO1996021024A1 | 1996-07-11 |
Other References:
FANG, G. & GRUMET, R.: "Genetic engineering of potyvirus resistance using constructs derived from the Zucchini yellow mosaic virus coat protein gene", MOL. PLANT-MICROBE INTERACTIONS, vol. 6, 1993, pages 358 - 367, XP002064882
SULLENGER, B. & CECH, T.: "TETHERING RIBOZYMES TO A RETROVIAL PACKAGING SIGNAL FOR DESTRUCTION OF VIRAL RNA", SCIENCE, vol. 262, 3 December 1993 (1993-12-03), pages 1566 - 1569, XP000567869
GADANI, F. ET AL.: "Genetic engineering of plants for virus resistance.", ARCHIVES OF VIROLOGY, vol. 115, 1990, pages 1 - 21, XP002064877
SAWYER, B. & BIRCH, R.: "A generic transient assay for antisense and ribozyme inhibition in vivo", PROCEEDINGS OF THE AUSTRALIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 28, 1996, XP002073852
VAN DER VLUGT R A ET AL: "Complex formation determines the activity of ribozymes directed against potato virus YN genomic RNA sequences.", VIRUS RESEARCH, (1993 FEB) 27 (2) 185-200., XP002064878
TURPEN T: "Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of poty viruses", J.GEN.VIROL.;(1989) 70, PT.8, 1951-6, XP002064879
EDINGTON, B. & NELSON, R.: "Utilization of ribozymes in plants: Plant viral resistance", ERICKSON, R. & IZANT, J. 'GENE REGULATION: BIOLOGY OF ANTISENSE RNA AND DNA'; RAVEN PRESS, NEW YORK, US; 1992, pages 209 - 221, XP002064880
SULLENGER, B. & CECH, T.: "TETHERING RIBOZYMES TO A RETROVIAL PACKAGING SIGNAL FOR DESTRUCTION OF VIRAL RNA", SCIENCE, vol. 262, 3 December 1993 (1993-12-03), pages 1566 - 1569, XP000567869
GADANI, F. ET AL.: "Genetic engineering of plants for virus resistance.", ARCHIVES OF VIROLOGY, vol. 115, 1990, pages 1 - 21, XP002064877
SAWYER, B. & BIRCH, R.: "A generic transient assay for antisense and ribozyme inhibition in vivo", PROCEEDINGS OF THE AUSTRALIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 28, 1996, XP002073852
VAN DER VLUGT R A ET AL: "Complex formation determines the activity of ribozymes directed against potato virus YN genomic RNA sequences.", VIRUS RESEARCH, (1993 FEB) 27 (2) 185-200., XP002064878
TURPEN T: "Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of poty viruses", J.GEN.VIROL.;(1989) 70, PT.8, 1951-6, XP002064879
EDINGTON, B. & NELSON, R.: "Utilization of ribozymes in plants: Plant viral resistance", ERICKSON, R. & IZANT, J. 'GENE REGULATION: BIOLOGY OF ANTISENSE RNA AND DNA'; RAVEN PRESS, NEW YORK, US; 1992, pages 209 - 221, XP002064880
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