Title:
SECRETED AND CYTOPLASMIC TUMOR ENDOTHELIAL MARKERS
Document Type and Number:
WIPO Patent Application WO2004005883
Kind Code:
A3
Abstract:
To gain a better understanding of tumor angiogenesis, new techniques for isolating endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, over 170 genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed many differentially expressed genes, including a large nujber of genes that were specifically elevated in tumor-associated endothelium. Experiments with representative genes from this group demonstrated that most were similarly expressed in the endothelium of primary lung, breast, brain, and pancreatic cancers as well as in metastatic lesions fo the liver. Theses results demonstrate that neoplastic and normal endothelium in humans are distinct at the molecular level, and have significant implications for the development of anti-angiogenic.
Inventors:
ST CROIX BRAD (US)
KINZLER KENNETH W (US)
VOGELSTEIN BERT (US)
KINZLER KENNETH W (US)
VOGELSTEIN BERT (US)
Application Number:
PCT/US2003/016250
Publication Date:
July 27, 2006
Filing Date:
July 02, 2003
Export Citation:
Assignee:
UNIV JOHNS HOPKINS (US)
ST CROIX BRAD (US)
KINZLER KENNETH W (US)
VOGELSTEIN BERT (US)
ST CROIX BRAD (US)
KINZLER KENNETH W (US)
VOGELSTEIN BERT (US)
International Classes:
A61K39/395; A61K38/00; A61K38/17; A61K38/18; A61K38/19; A61K38/39; A61K38/44; A61K38/47; A61K38/48; A61K38/49; A61K38/55; C12Q1/68; G01N33/50; G01N33/53; G01N33/574
Domestic Patent References:
| WO2002010217A2 | 2002-02-07 |
Other References:
ST. CROIX ET AL.: "Genes Expressed in Human Tumor Endothelium", SCIENCE, 18 August 2000 (2000-08-18), pages 1197 - 1202, XP002222041, DOI: doi:10.1126/science.289.5482.1197
NOVATCHKOVA ET AL.: "Can molecular mechanisms of biological processes be extracted from expression profiles?", BIOESSAYS, vol. 23, 2001, pages 1159 - 1175
CARSON-WALTER ET AL.: "Cell Surface Tumor Endothelial Markers Are Conserved in Mice and Humans", CANCER RESEARCH, vol. 61, 15 September 2001 (2001-09-15), pages 6649 - 6655
WANG ET AL.: "Identification of Tumor Angiogenesis-Related Genes by Subtractive Hybridization", MICROVASCULAR RESEARCH, vol. 59, 2000, pages 394 - 397, XP002372830, DOI: doi:10.1006/mvre.2000.2242
NOVATCHKOVA ET AL.: "Can molecular mechanisms of biological processes be extracted from expression profiles?", BIOESSAYS, vol. 23, 2001, pages 1159 - 1175
CARSON-WALTER ET AL.: "Cell Surface Tumor Endothelial Markers Are Conserved in Mice and Humans", CANCER RESEARCH, vol. 61, 15 September 2001 (2001-09-15), pages 6649 - 6655
WANG ET AL.: "Identification of Tumor Angiogenesis-Related Genes by Subtractive Hybridization", MICROVASCULAR RESEARCH, vol. 59, 2000, pages 394 - 397, XP002372830, DOI: doi:10.1006/mvre.2000.2242
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