CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims benefit to U.S. Provisional Application Serial Number 60/638,315, filed December 22, 2004, incorporated herein by reference in its entirety.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR

DEVELOPMENT

The research and development of the invention described below was not federally sponsored.

BACKGROUND

The term "opiate" has been used to designate pharmacologically active alkaloids derived from opium, e.g., morphine, codeine, and many semi-synthetic congeners of morphine. After the isolation of peptide compounds with morphine-like actions, the term opioid was introduced to refer generically to all drugs with morphine-like actions. Included among opioids are various peptides that exhibit morphine-like activity, such as endorphins, enkephalins and dynorphins. However, some sources use the term "opiate" in a generic sense, and in suclVcontexts, opiate and opioid are interchangeable. Additionally, the term opioid has been used to refer to antagonists of morphine-like drugs as well as to characterize receptors or binding sites that combine with such agents.

Opioids are generally employed as analgesics, but they may have many other pharmacological effects as well. Morphine and related opioids produce certain of their major effects on the central nervous and digestive systems. The effects are diverse, including analgesia, drowsiness, mood changes, respiratory depression, dizziness, mental clouding, dysphoria, pruritus, increased pressure

in the biliary tract, decreased gastrointestinal motility, nausea, vomiting, and alterations of the endocrine and autonomic nervous systems.

When therapeutic doses of morphine are given to patients with pain, they report that the pain is less intense, less discomforting, or entirely gone. In addition to experiencing relief of distress, some patients experience euphoria. However, when morphine in a selected pain-relieving dose is given to a pain-free individual, the experience is not always pleasant; nausea is common, and vomiting may also occur. Drowsiness, inability to concentrate, difficulty in mentation, apathy, lessened physical activity, reduced visual acuity, and lethargy may ensue.

Two distinct classes of opioid molecules can bind opioid receptors: the opioid peptides (e.g., the enkephalins, dynorphins, and endorphins) and the alkaloid opiates (e.g., morphine, etorphine, diprenorphine and naloxone). Subsequent to the initial demonstration of opiate binding sites (Pert, C. B. and Snyder, S. H., Science (1973) 179:1011-1014), the differential pharmacological and physiological effects of both opioid peptide analogues and alkaloid opiates served to delineate multiple opioid receptors. Accordingly, three molecularly and pharmacologically distinct opioid receptor types have been described: delta, kappa and mu. Furthermore, each type is believed to have sub-types (Wollemann, M., J Neurochem (1990) 54:1095-1101 ; Lord, J. A., et al., Nature (1977) 267:495-499).

All three of these opioid receptor types appear to share the same functional mechanisms at a cellular level. For example, the opioid receptors cause inhibition of adenylate cyclase, and inhibition of neurotransmitter release via both potassium channel activation and inhibition of Ca ²⁺ channels (Evans, C. J., In: Biological Basis of Substance Abuse, S. G. Korenman & J. D. Barchas, eds., Oxford University Press (in press); North, A. R., et al., Proc Natl Acad Sci USA (1990) 87:7025-29; Gross, R. A., et al., Proc Natl Acad Sci USA (1990) 87:7025-29; Sharma, S. K., et al., Proc Natl Acad Sci USA (1975) 72:3092-96). Although the functional mechanisms are the same, the behavioral manifestations of receptor-selective drugs differ greatly (Gilbert, P. E. & Martin,

W. R., J Pharmacol Exp Ther (1976) 198:66-82). Such differences may be attributable in part to the anatomical location of the different receptors.

Delta receptors have a more discrete distribution within the mammalian CNS than either mu or kappa receptors, with high concentrations in the amygdaloid complex, striatum, substantia nigra, olfactory bulb, olfactory tubercles, hippocampal formation, and the cerebral cortex (Mansour, A., et al., Trends in Neurosci (1988) 11 :308-14). The rat cerebellum is remarkably devoid of opioid receptors including delta opioid receptors.

D. Delorme, E. Roberts and Z _L Wei, World Patent WO/28275 (1998) discloses diaryl methylidenylpiperidines that are opioid analgesics, but does not disclose or suggest the compounds of the present invention.

C. Kaiser, and others (J. Med. Chem. 1974, Volume 17, pages 57-61 ) disclose some piperidylidene derivatives of thioxanthenes, xanthenes, dibenoxepins and acridans that are neuroleptic agents. These authors, however, do not disclose or suggest either the structure or the activity of the compounds of the present invention.

British Patent GB 1128734 (1966) discloses derivatives of 6,11-dihydrodibenzo[b,e]oxepine that are anticholinergic, anti-convulsive, muscle-relaxing, sedating, diuretic, and/or vasoactive agents. These, agents, however, differ significantly from the compounds of the present invention both structurally and pharmacologically.

There is a continuing need for new delta opioid receptor modulators as analgesics. There is a further need for delta opioid receptor selective agonists as analgesics having reduced side effects. There is also a need for delta opioid receptor antagonists as immunosuppressants, antiinflammatory agents, agents for the treatment of neurological and psychiatric conditions, agents for the treatment of urological and reproductive conditions, medicaments for drug and alcohol abuse, agents for treating gastritis and diarrhea, cardiovascular agents and agents for the treatment of respiratory diseases, having reduced side effects.

SUMMARY

The present invention is directed, inter alia, to compounds of Formula (I) and compositions comprising a compound of Formula (I):

Formula (I) wherein:

G is -C(Z)N(Ri)R ₂ , C _6- i _O aryl, or a heterocycle selected from the group consisting of imidazolyl, triazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, imidazolinyl, tetrahydropyrimidinyl, thienyl, pyrazolyl, pyrimidinyl, triazinyl, furyl, indazolyl, indolyl, indolinyl, isothiazolyl, isoxazolyl, oxazolyl, isoxadiazolyl, benzoxazolyl, quinolinyl, isoquinolinyl, and pyridinyl; wherein aryl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of C _1-8 alkanyl, C _2- salkenyl, C^-salkynyl, C<|. ₈ alkanyloxy, hydroxy(Ci- ₈ )alkanyl, carboxy(C ₁ - ₈ )alkanyl, Ci-salkanylcarbonylamino, halogen, hydroxy, cyano, nitro, oxo, thioxo, amino, Ci- ₆ alkanylamino, di(Ci _-6 alkanyl)amino, Ci- ₈ alkanylthio, Ci- ₈ alkanylsulfonyl, C-ι_ ₈ alkanylsulfonylamino, ) aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, Ci-salkanylaminocarbonyl, di(Ci _-8 alkanyl)aminocarbonyl, and C-i-ealkanyloxycarbonylamino;

Ri is a substituent selected from the group consisting of hydrogen, Ci _-8 alkanyl, C _2-8 alkenyl, and C _2-8 alkynyl;

R ₂ is a substituent selected from the group consisting of hydrogen; Ci _-8 alkanyl; C _2-8 alkenyl; C _2-8 alkynyl; C ₆ -ioaryl; and

Ci- ₈ cycloalkanyl; wherein Ci _-8 alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, amino, Ci _-6 alkanylamino, di(Ci _-6 alkanyl)amino, Ci _-6 alkanyloxy, thioCi _-6 alkanyloxy, hydroxy, fluoro, chloro, cyano, aminocarbonyl, C-i-βalkanylaminocarbonyl, di(Ci. ₈ alkanyl)aminocarbonyl, C-i _-6 alkanyloxycarbonyl, and aryloxy; and wherein any aryl-containing substituents and Ci _-8 cycloalkanyl substituents of R ₂ are optionally substituted with one to three substituents independently selected from the group consisting of C-ι _-8 alkanyl, C- _2-8 alkenyl, C _2-8 alkynyl, Ci _-8 alkanyloxy, trifluoromethyl, trifluoromethoxy, phenyl, halogen, cyano, hydroxy, Ci _-8 alkanylthio, Ci _-8 alkanylsulfonyl, and Ci _-8 alkanylsulfonylamino; or Ri and R ₂ taken together with the nitrogen to which they are attached form a 5-7 membered cycloheteroalkyl optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, hydroxy(Ci _-8 )alkanyl, hydroxy, amino, C-i-βalkanylamino, di(Ci- ₆ alkanyl)amino, and halogen;

R ₃ is a substituent selected from the group consisting of hydrogen, Ci _-8 alkanyl, haloi _-3 (C"i _-8 )alkanyl, C _2-8 alkenyl, C _2-8 alkynyl, C- ₃ - _δ Cycloalkanyl, cycloalkanyl(Ci _-8 )alkanyl, Ci _-8 alkanyloxy(Ci _-8 )alkanyl, Ci _-8 alkanylthio(Ci _-8 )alkanyl, hydroxyCi _-8 alkanyl, Ci-salkanyloxycarbonyl, halθi_ ₃ (Ci _-8 )alkanylcarbonyl, formyl, thioformyl, carbamimidoyl, phenylimino(Ci _-8 )alkanyl, phenyl(Ci _-8 )alkanyl, phenyl(Ci _-8 )aikenyl, phenyl(Ci _-8 )alkynyl, naphthyl(Ci _-8 )alkanyl and heteroaryl(Ci _-8 )alkanyl wherein the heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, thienyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, pyrrolyl, quinolinyl, isoquinolinyl, tetrazolyl, thiazolyl; wherein phenyl, naphthyl and

heteroaryl are optionally substituted with one to three substituents independently selected from the group consisting of C-i-βalkanyl, C _2-6 aIkenyl, C-i-βalkanyloxy, amino, d-βalkanylamino, di(Ci _-6 alkanyl)amino, Ci _-6 alkanylcarbonyl, Ci _-6 alkanylcarbonyloxy, Ci _-6 alkanylcarbonylamino, Ci _-6 alkanylthio, C-i-βalkanylsulfonyl, halogen, hydroxy, cyano, fluoro(Ci _-6 )alkanyl, thioureido, and fluoro(Ci _-6 )alkanyloxy; alternatively, when phenyl and heteroaryl are optionally substituted with alkanyl or alkanyloxy substituents attached to adjacent carbon atoms, the two substituents can together form a fused cyclic alkanyl or cycloheteroalkanyl selected from the group consisting of -(0^)3.5- , -O(CH ₂ )2-4- , -(CH ₂ ) ₂ - ₄ O -, and -O(CH ₂ )i _-3 O-;

R ₄ is one to three substituents independently selected from the group consisting of hydrogen; Ci _-6 alkanyl; C _2-6 alkenyl; C _2-6 alkynyl; aryl(C _2-6 )alkynyl; C-i-βalkanyloxy; amino; Ci-βalkanylamino; di(Ci- ₆ alkanyl)amino; C _6- ioarylamino wherein C _6- ioaryl is optionally substituted with one to three substitutents independently selected from the group consisting of C-i-βalkanyl, Ci-βalkoxy, halogen, and hydroxyl; formylamino; pyridinylamino; d-βalkanylcarbonyl; C _1-6 alkanylcarbonyloxy; Ci _-6 alkanyloxycarbonyl; aminocarbonyl; C _1-6 alkanylaminocarbonyl; di(Ci_ ₆ alkanyl)aminocarbonyl; Ci-βalkanylcarbonylamino; C _1-6 alkanylthio; Ci- ₆ alkanylsulfonyl; halogen; hydroxy; cyano; hydroxycarbonyl; Cβ-ioaryl; chromanyl; chromenyl; furanyl; imidazolyl; indazolyl; indolyl; indolinyl; isoindolinyl; isoquinolinyl; isothiazolyl; isoxazolyl; naphthyridinyl; oxazolyl; pyrazinyl; pyrazolyl; pyridazinyl; pyridinyl; pyrimidinyl; pyrrolyl; quinazolinyl; quinolinyl; quinolizinyl; quinoxalinyl; tetrazolyl; thiazolyl; thienyl; fluoroalkanyl and fluoroalkanyloxy; or optionally, when R ₄ is two substituents attached to adjacent carbon atoms, the two substituents together form a single fused

moiety; wherein the fused moiety is -(CH ₂ )3-5-, -O(CH ₂ )2-4- , -(CH ₂ )S _-4 O-, -0(CH ₂ ) _I-3 O-, or - 3-C(NH ₂ )=N-;

R ₅ is one to two substituents independently selected from the group consisting of hydrogen, Ci-βalkanyl, C ₂ _ ₆ alkenyl, Ci- ₆ alkanyloxy, amino, C _1-6 alkanylamino, di(Ci _-6 alkanyl)amino, C _1-6 alkanylcarbonyl, Ci _-6 alkanylcarbonyloxy, Ci-βalkanyloxycarbonyl, C-i-ealkanylaminocarbonyl, Ci-ealkanylcarbonylamino, C-i-βalkanylthio, Ci_ ₆ alkanylsulfonyl, halogen, hydroxy, cyano, fluoro(Ci- ₆ )alkanyl and fluoro(Ci. β)alkanyloxy;

A is -(CH ₂ ) _m -, wherein m is 2 or 3;

Y is O or S;

Z is O, S, NH, N(C _1-6 alkanyl), N(OH), N(OC _1-6 alkanyl), or N(phenyl); and enantiomers, diastereomers, tautomers, solvates, or pharmaceutically acceptable salts thereof.

The present invention is also directed to, inter alia, veterinary and pharmaceutical compositions containing compounds of Formula (I) wherein the compositions are used to treat mild to severe pain in warm-blooded animals.

DETAILED DESCRIPTION

As used herein, the following underlined terms are intended to have the following meanings:

"C _a ^" (where a and b are integers) refers to a radical containing from a to h carbon atoms inclusive. For example, Ci_ ₃ denotes a radical containing 1 , 2 or 3 carbon atoms

"Alkyl:" refers to a saturated or unsaturated, branched, straight-chain or cyclic monovalent hydrocarbon radical derived by the removal of one hydrogen

atom from a single carbon atom of a parent alkane, alkene or alkyne. Typical alkyl groups include, but are not limited to, methyl; ethyls such as ethanyl, ethenyl, ethynyl; propyls such as propan-1-yl, propan-2-yl , cyclopropan-1-yl, prop-1-en~1-yl, prop-1-en-2-yl, prop-2-en-1-yl, cycloprop-1~en-1-yl; cycloprop-2-en-1-yl, prop-1-yl)-1-yl, prop-2-yl)-1-yl, etc.; butyls such as butan-1-yl, butan-2-yl, 2-methyl-propan-1-yl, 2-methyl-propan-2-yl, cycIobutan-1-yl, but-1-en-1-yl, but-1-en-2-yl, 2-methyl-prop-1-en-1-yl, but-2-en-1-yl, but-2-en-2-yl, buta-1 ,3-dien-1-yl, buta-1 ,3-dien-2-yl, cyclobut-1-en-1-yl, cyclobut-1-en-3-yl, cyclobuta-1 ,3-dien-1-yl, but-1-yl)-1-yl, but-1-yl)-3-yl, but-3-yl)-1-yl, etc.; and the like. Where specific levels of saturation are intended, the nomenclature "alkanyl", "alkenyl" and/or "alkynyl" is used, as defined below. In preferred embodiments, the alkyl groups are (Ci-C ₆ ) alkyl, with (C- ₁ -C3) being particularly preferred.

"Alkanyl:" refers to a saturated branched, straight-chain or cyclic monovalent hydrocarbon radical derived by the removal of one hydrogen atom from a single carbon atom of a parent alkane. Typical alkanyl groups include, but are not limited to, methanyl; ethanyl; propanyls such as propan-1-yl, propan-2-yl, cyclopropan-1-yl, etc.; butyanyls such as butan-1-yl, butan-2-yl, 2-methyl-propan-1-yl, 2-methyl-propan-2-yl, cyclobutan-1-yl, etc.; and the like. In preferred embodiments, the alkanyl groups are (Ci _-8 ) alkanyl, with (C ₁ - ₃ ) being particularly preferred.

"Alkenyl" refers to an unsaturated branched, straight-chain or cyclic monovalent hydrocarbon radical having at least one carbon-carbon double bond derived by the removal of one hydrogen atom from a single carbon atom of a parent alkene. The radical may be in either the cis or trans conformation about the double bond(s). Typical alkenyl groups include, but are not limited to, ethenyl; propenyls such as prop-1-en-1-yl, prop-1-en-2-yl, prop-2-en-1-yl, prop-2-en-2-yl, cycloprop-1-en-1-yl; cycloprop-2-en-i-yl; butenyls such as but-1-en-1-yl, but-1-en-2-yl, 2-methyl-prop-1-en-1-yl, but-2-en-1-yl,

but-2-en-1-yl, but-2-en-2-yl, buta-1 ,3-dien-1-yl, buta-1 ,3-dien-2-yl, cyclobut-1-en-1-yl, cyclobut-1-en-3-yl, cyclobuta-1 ,3-dien-1-yl, etc.; and the like.

"Alkvnyl" refers to an unsaturated branched, straight-chain or cyclic monovalent hydrocarbon radical having at least one carbon-carbon triple bond derived by the removal of one hydrogen atom from a single carbon atom of a parent alkyne. Typical alkynyl groups include, but are not limited to, ethynyl; propynyls such as prop-1-yl)-1-yl, prop-2-yl)-1-yl, etc.; butynyls such as but-1-yl)-1-yl, but-1-yl)-3-yl, but-3-yl)-1-yl, etc.; and the like.

"Heteroalkyl" and Heteroalkanyl" refer to alkyl or alkanyl radicals, respectively, in which one or more carbon atoms (and any necessary associated hydrogen atoms) are independently replaced with the same or different heteroatoms (including any necessary hydrogen or other atoms). Typical heteroatoms to replace the carbon atom(s) include, but are not limited to, N, P, O, S, Si, etc. Preferred heteroatoms are O, N and S. Thus, heteroalkanyl radicals can contain one or more of the same or different heteroatomic groups, including, by way of example and not limitation, epoxy (-O-), epidioxy (-O-O-), thioether (-S-), epidithio (-SS-), epoxythio (-O-S-), epoxyimino (-O-NR ¹ -), imino (-NR ¹ -), biimino (-NR'-NR ¹ -), azino (=N-N=), azo (-N=N-), azoxy (-N-O-N-), azimino (-NR'-N=N-), phosphano (-PH-), λ ⁴ -sulfano (-SH ₂ -), sulfonyl (-3(O) ₂ -), and the like, where each R' is independently hydrogen or (Ci-Cβ) alkyl.

"Parent Aromatic Ring System:" refers to an unsaturated cyclic or , polycyclic ring system having a conjugated π electron system. Specifically included within the definition of "parent aromatic ring system" are fused ring systems in which one or more rings are aromatic and one or more rings are saturated or unsaturated, such as, for example, indane, indene, phenalene, etc. Typical parent aromatic ring systems include, but are not limited to, aceanthrylene, acenaphthylene, acephenanthrylene, anthracene, azulene,

benzene, chrysene, coronene, fluoranthene, fluorene, hexacene, hexaphene, hexalene, as-indacene, s-indacene, indane, indene, naphthalene, octacene, octaphene, octalene, ovalene, penta-2,4-diene, pentacene, pentalene, pentaphene, perylene, phenalene, phenanthrene, picene, pleiadene, pyrene, pyranthrene, rubicene, triphenylene, trinaphthalene, and the like

"Aryl:" refers to a monovalent aromatic hydrocarbon radical derived by the removal of one hydrogen atom from a single carbon atom of a parent aromatic ring system. Typical aryl groups include, but are not limited to, radicals derived from aceanthrylene, acenaphthylene, acephenanthrylene, anthracene, azulene, benzene, chrysene, coronene, fluoranthene, fluorene, hexacene, hexaphene, hexalene, as-indacene, s-indacene, indane, indene, naphthalene, octacene, octaphene, octalene, ovalene, penta-2,4~diene, pentacene, pentalene, pentaphene, perylene, phenalene, phenanthrene, picene, pleiadene, pyrene, pyranthrene, rubicene, triphenylene, trinaphthalene, and the like. In preferred embodiments, the aryl group is (C5-2 ₀ ) aryl, with (C5- ₁ 0) being particularly preferred. Particularly preferred aryl groups are phenyl and naphthyl groups.

"Arylalkyl:" refers to an acyclic alkyl group in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal carbon atom, is replaced with an aryl radical. Typical arylalkyl groups include, but are not limited to, benzyl, 2-phenylethan-1-yl, 2-phenylethen-i-yl, naphthylmethyl, 2-naphthylethan-1-yl, 2-naphthylethen-1-yl, naphthobenzyl, 2-naphthophenylethan-1-yl and the like. Where specific alkyl moieties are intended, the nomenclature arylalkanyl, arylakenyl and/or arylalkynyl is used. [In preferred embodiments, the arylalkyl group is (C ₆ - ₂₆ ) arylalkyl, e.g., the alkanyl, alkenyl or alkynyl moiety of the arylalkyl group is (C-ι- ₆ ) and the aryl moiety is (C ₅ - ₂ o). In particularly preferred embodiments the arylalkyl group is (C- ₆ - ₁₃ ). e.g., the alkanyl, alkenyl or alkynyl moiety of the arylalkyl group is (C1- ₃ )

and the aryl moiety is (C ₅ . ₁₀ ). Even more preferred arylalkyl groups are phenylalkanyls.

"Alkanyloxy:" refers to a saturated branched, straight-chain or cyclic monovalent hydrocarbon alcohol radical derived by the removal of the hydrogen atom from the hydroxide oxygen of the alcohol. Typical alkanyloxy groups include, but are not limited to, methanyloxy; ethanyloxy; propanyloxy groups such as propan-1 -yloxy (CH ₃ CH ₂ CH ₂ O-), propan-2-yloxy ((CHs) ₂ CHO-), cyclopropan-1 -yloxy, etc.; butanyloxy groups such as butan-1 -yloxy, butan-2-yloxy, 2-methyl-propan-1 -yloxy, 2-methyl-propan-2-y!oxy, cyclobutan-1 -yloxy, etc.; and the like. In preferred embodiments, the alkanyloxy groups are (C-i-s) alkanyloxy groups, with (C ₁ - ₃ ) being particularly preferred.

"Parent Heteroaromatic Ring System:" refers to a parent aromatic ring system in which one carbon atom is replaced with a heteroatom. Heteratoms to replace the carbon atoms include N, O, and S. Specifically included within the definition of "parent heteroaromatic ring systems" are fused ring systems in which one or more rings are aromatic and one or more rings are saturated or unsaturated, such as, for example, arsindole, chromane, chromene, indole, indoline, xanthene, etc. Typical parent heteroaromatic ring systems include, but are not limited to, carbazole, imidazole, indazole, indole, indoline, indolizine, isoindole, isoindoline, isoquinoline, isothiazole, isoxazole, naphthyridine, oxadiazole, oxazole, purine, pyran, pyrazine, pyrazole, pyridazine, pyridine, pyrimidine, pyrrole, pyrrolizine, quinazoline, quinoline, quinolizine, quinoxaline, tetrazole, thiadiazole, thiazole, thiophene, triazole, xanthene, and the like.

"Heteroaryl:" refers to a monovalent heteroaromatic radical derived by the removal of one hydrogen atom from a single atom of a parent heteroaromatic ring system. Typical heteroaryl groups include, but are not

limited to, radicals derived from carbazole, imidazole, indazole, indole, indoline, indolizine, isoindole, isoindoline, isoquinoline, isothiazole, isoxazole, naphthyridine, oxadiazole, oxazole, purine, pyran, pyrazine, pyrazole, pyridazine, pyridine, pyrimidine, pyrrole, pyrrolizine, quinazoline, quinoline, quinolizine, quinoxaline, tetrazole, thiadiazole, thiazole, thiophene, triazole, xanthene, and the like. In preferred embodiments, the heteroaryl group is a 5-20 membered heteroaryl, with 5-10 membered heteroaryl being particularly preferred.

"Cvcloheteroalkyl:" refers to a saturated or unsaturated monocyclic or bicyclic alkyl radical in which one carbon atom is replaced with N, O or S. In certain specified embodiments the cycloheteroalkyl may contain up to four heteroatoms independently selected from N, O or S. Typical cycloheteroalkyl moieties include, but are not limited to, radicals derived from imidazolidine, morpholine, piperazine, piperidine, pyrazolidine, pyrrolidine, quinuclidine, and the like. In preferred embodiments, the cycloheteroalkyl is a 3-6 membered cycloheteroalkyl.

"Cvcloheteroalkanyl:" refers to a saturated monocyclic or bicyclic alkanyl radical in which one carbon atom is replaced with N, O or S. In certain specified embodiments the cycloheteroalkanyl may contain up to four heteroatoms independently selected from N ₁ O or S. Typical cycloheteroalkanyl moieties include, but are not limited to, radicals derived from imidazolidine, morpholine, piperazine, piperidine, pyrazolidine, pyrrolidine, quinuclidine, and the like. In preferred embodiments, the cycloheteroalkanyl is a 3-6 membered cycloheteroalkanyl.

"Cvcloheteroalkenyl:" refers to a saturated monocyclic or bicyclic alkenyl radical in which one carbon atom is replaced with N, O or S. Ih certain specified embodiments the cycloheteroalkenyl may contain up to four heteroatoms independently selected from N ₁ O or S. Typical cycloheteroalkenyl moieties include, but are not limited to, radicals derived from imidazoline,

pyrazoline, pyrroline, indoline, pyran, and the like. In preferred embodiments, the cycloheteroalkanyl is a 3-6 membered cycloheteroalkanyl.

"Substituted:" refers to a radical in which one or more hydrogen atoms are each independently replaced with the same or different substituent(s). Typical substituents include, but are not limited to, -X, -R, -O ^" , =O, -OR, _O-OR, -SR, - 3-, =S, -NRR, =NR, -CX ₃ , -CN, -OCN, -SCN, -NCO, -NCS, -NO, -NO ₂ , =N ₂ , -N ₃ , -NHOH, -S(O) ₂ O-, -S(O) ₂ OH, -S(O) ₂ R, -P(OXO) ₂ , -P(O)(OH) ₂ , -C(O)R ₁ -C(O)X, -C(S)R, -C(S)X, -C(O)OR, -C(O)O ^' , -C(S)OR, -C(O)SR, -C(S)SR, -C(O)NRR, -C(S)NRR and -C(NR)NRR, where each X is independently a halogen (preferably -F, -Cl or -Br) and each R is independently -H, alkyl, alkanyl, alkenyl, alkynyl, alkylidene, alkylidyne, aryl, arylalkyl, arylheteroalkyl, heteroaryl, heteroarylalkyl or heteroaryl-heteroalkyl, as defined herein. Preferred substituents include hydroxy, halogen, Ci _-8 alkyl, C-i- ₈ alkanyloxy, fluorinated alkanyloxy, fluorinated alkyl, C-|. ₈ alkylthio, C _3-8 cycloalkyl, C _3-8 cycloalkanyloxy, nitro, amino, C-i-salkylamino, Ci _-8 dialkylamino, Cs-βcycloalkylamino, cyano, carboxy, Ci _-7 alkanyloxycarbonyl, Ci _-7 alkylcarbonyloxy, formyl, carbamoyl, phenyl, aroyl, carbamoyl, amidino, (Ci _-8 alkylamino)carbonyl, (arylamino)carbonyl and aryl(Ci _-8 alkyl)carbonyl.

With reference to substituents, the term "independently" means that when more than one of such substituent is possible, such substituents may be the same or different from each other.

Throughout this disclosure, the terminal portion of the designated side chain is described first, followed by the adjacent functionality toward the point of attachment. Thus, for example, a "phenylCi-ealkanylaminocarbonylCi-βalkyl" substituent refers to a group of the formula

alkanyl alkanyl

005/046691

The present invention is directed, inter alia, to compounds of Formula (I) and compositions comprising a compound of Formula (I):

Formula (I) wherein:

G is -C(Z)N(Ri)R ₂ , C ₆ --ιoaryl, or a heterocycle selected from the group consisting of imidazolyl, triazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, imidazolinyl, tetrahydropyrimidinyl, thienyl, pyrazolyl, pyrimidinyl, triazinyl, furyl, indazolyl, indolyl, indolinyl, isothiazolyl, isoxazolyl, oxazolyl, isoxadiazolyl, benzoxazolyl, quinolinyl, isoquinolinyl, and pyridinyl; wherein aryl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of C-i-salkanyl, C ₂ - ₈ alkenyl, C _2- salkynyl, d-salkanyloxy, hydroxy(Ci_ ₈ )alkanyl, carboxy(Ci- ₈ )alkanyl, Ci-salkanylcarbonylamino, halogen, hydroxy, cyano, nitro, oxo, thioxo, amino, Ci-βalkanylamino, di(C _1-6 alkanyl)amino, Ci _-8 alkanylthio, C _1-8 alkanylsulfonyl, C _1-8 alkanylsulfonylamino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, Ci-salkanylaminocarbonyl, di(Ci. ₈ alkanyl)aminocarbonyl, and Ci-βalkanyloxycarbonylamino;

Ri is a substituent selected from the group consisting of hydrogen, d-salkanyl, C _2-8 alkenyl, and C _2-8 alkynyl;

R2 is a substituent selected from the group consisting of hydrogen; Ci-aalkanyl; C _2-8 alkenyl; C _2-8 alkynyl; C ₆ -ioaryl; and Ci-scycloalkanyl; wherein Ci-salkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, amino, Ci _-6 alkanylamino, di(Ci_6aIkanyl)amino, Ci-βalkanyloxy, thioC-i-βalkanyloxy, hydroxy, fluoro, chloro, cyano, aminocarbonyl, C-i-aalkanylaminocarbonyl, di(Ci- ₈ alkanyl)aminocarbonyl, d-βalkanyloxycarbonyl, and aryloxy; and wherein any aryl-containing substituents and Ci-scycloalkanyl substituents of R ₂ are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, C _2-8 alkenyl, C _2-8 alkynyl, Ci. ₈ alkanyloxy, trifluoromethyl, trifluoromethoxy, phenyl, halogen, cyano, hydroxy, C-i-salkanylthio, Ci _-8 alkanylsulfonyl, and Ci _-8 alkanylsulfonylamino; or Ri and R ₂ taken together with the nitrogen to which they are attached form a 5-7 membered cycloheteroalkyl optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, hydroxy(Ci _-8 )alkanyl, hydroxy, amino, Ci _-6 alkanylamino, di(Ci _-6 alkanyl)amino, and halogen;

R ₃ is a substituent selected from the group consisting of hydrogen, Ci _-8 alkanyl, haloi- ₃ (Ci- ₈ )alkanyl, C _2-8 alkenyI, C _2-8 alkynyl, C _3-8 cycloalkanyl, cycloalkanyl(Ci _-8 )alkanyl, Ci _-8 alkanyloxy(Ci. ₈ )alkanyl, C ₁ _ ₈ alkanylthio(Ci _-8 )alkanyl, hydroxyd-salkanyl, Ci-salkanyloxycarbonyl, haloi _-3 (Ci _-8 )alkanylcarbonyl, formyl, thioformyl, carbamimidoyl, phenylimino(Ci _-8 )alkanyl, phenyl(Ci _-8 )alkanyl, phenyl(Ci _-8 )alkenyl, phenyl(Ci_ ₈ )alkynyl, naphthyl(C-i- ₈ )alkanyl and heteroaryl(Ci- ₈ )alkanyl wherein the heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl,

pyridinyl, thienyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, pyrrolyl, quinolinyl, isoquinolinyl, tetrazolyl, thiazolyl; wherein phenyl, naphthyl and heteroaryl are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-6 alkanyl, C _2- 6alkenyI, Ci _-6 alkanyloxy, amino, C _1-6 alkanylamino, di(C _1-6 alkanyl)amino, Ci_ ₆ alkanylcarbonyl, C _1-6 alkanylcarbonyloxy, C-i-βalkanylcarbonylamino, Ci _-6 alkanylthio, Ci _-6 alkanylsulfonyl, halogen, hydroxy, cyano, fluoro(Ci- ₆ )alkanyl, thioureido, and fluoro(Ci _-6 )alkanyloxy; alternatively, when phenyl and heteroaryl are optionally substituted with alkanyl or alkanyloxy substituents attached to adjacent carbon atoms, the two substituents can together form a fused cyclic alkanyl or cycloheteroalkanyl selected from the group consisting of -(CH2)3-5- , -O(CH2)2-4- _> - (CH ₂ ) _2-4 O -, and -O(CH ₂ ) _1-3 O-;

R ₄ is one to three substituents independently selected from the group consisting of hydrogen; Ci _-6 alkanyl; C _2-6 alkenyl; C _2-6 alkynyI; aryl(C _2-6 )alkynyl; C-i-βalkanyloxy; amino; Ci-βalkanylamino; di(Ci _-6 alkanyl)amino; Cβ-ioarylaniino wherein C ₆ -ioaryl is optionally substituted with one to three substitutents independently selected from the group consisting of Ci _-6 alkanyl, Ci _-6 alkoxy, halogen, and hydroxyl; formylamino; pyridinylamino; Ci-βalkanylcarbonyl; Ci- ₆ alkanylcarbonyloxy; C-i-βalkanyloxycarbonyl; aminocarbonyl; Ci-βalkanylaminocarbonyl; di(Ci- ₆ alkanyl)aminocarbonyl; Ci-ealkanylcarbonylamino; C-i-βalkanylthio; Ci- ₆ alkanylsulfonyl; halogen; hydroxy; cyano; hydroxycarbonyl; C ₆ -ioaryl; chromanyl; chromenyl; furanyl; imidazolyl; indazolyl; indolyl; indolinyl; isoindolinyl; isoquinolinyl; isothiazolyl; isoxazolyl; naphthyridinyl; oxazolyl; pyrazinyl; pyrazolyl; pyridazinyl; pyridinyl; pyrimidinyl; pyrrolyl; quinazolinyl; quinolinyl; quinolizinyl; quinoxalinyl;

tetrazolyl; thiazolyl; thienyl; fluoroalkanyl and fluoroalkanyloxy; or optionally, when R ₄ is two substituents attached to adjacent carbon atoms, the two substituents together form a single fused moiety; wherein the fused moiety is -(CH ₂ )3-5-, -O(CH ₂ )2-4- , -(CH ₂ ) ₂ - ₄ θ-, -O(CH ₂ )i- ₃ O-, or-S-C(NH ₂ )=N-;

R ₅ is one to two substituents independently selected from the group consisting of hydrogen, Ci _-6 alkanyl, C _2-6 alkenyl, Ci _-6 alkanyloxy, amino, C-ι _-6 alkanylamino, di(Ci _-6 alkanyl)amino, Ci-βalkanylcarbonyl, Ci-βalkanylcarbonyloxy, d-βalkanyloxycarbonyl, C^ealkanylaminocarbonyl, Ci-βalkanylcarbonylamino, d-βalkanylthio, Ci _-6 alkanylsulfonyl, halogen, hydroxy, cyano, fluoro(Ci _-6 )alkanyl and fluoro(Ci- ₆ )alkanyloxy;

A is -(CH ₂ ) _m -, wherein m is 2 or 3;

Y is O or S;

Z is O, S, NH, N(Ci _-6 alkanyl), N(OH), N(OC _1-6 alkanyl), or N(phenyl); and enantiomers, diastereomers, tautomers, solvates, or pharmaceutically acceptable salts thereof.

An embodiment of the present invention is directed to a compound of Formula (I) wherein the structure is numbered as defined herein and the substituents are as defined herein.

Formula (I)

The present invention is directed, inter alia, to analgesic and anti-pyretic uses of compositions comprising a compound of Formula (I):

Formula (I)

wherein:

G is C ₆ -ioaryl, or a heterocycle selected from the group consisting of: imidazolyl, triazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, imidazolinyl, tetrahydropyrimidinyl, thienyl, pyrazolyl, pyrimidinyl, triazinyl, furyl, indazolyl, indolyl, indolinyl, isothiazolyl, isoxazolyl, oxazolyl, isoxadiazolyl, benzoxazolyl, quinolinyl, isoquinolinyl, and pyridinyl; wherein aryl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, C- _2-8 alkenyl, C _2-8 alkynyl, Ci _-8 alkanyloxy, hydroxy(Ci_ ₈ )alkanyl, carboxy(Ci- ₈ )alkanyl, Ci_ ₈ alkanylcarbonylamino, halogen, hydroxy, cyano, nitro, oxo, thioxo, amino, Ci- ₆ alkanylamino, di(Ci- ₆ alkanyl)amino, Ci _-8 alkanylthio, Ci _-8 alkanylsulfonyl, Ci _-8 alkanylsulfonylamino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, C-i-salkanylaminocarbonyl, di(Ci _-8 alkanyl)aminocarbonyl, and C-i-βalkanyloxycarbonylamino;

R ₁ is a substituent selected from the group consisting of hydrogen, C _1-8 alkanyl, C _2-8 alkenyl, and C _2-8 alkynyl;

US2005/046691

R ₂ is a substituent selected from the group consisting of hydrogen; C-i-salkanyl; C ₂ - ₈ alkenyl; C _2-8 alkynyl; C _6- ioaryl; and Ci-βcycloalkanyl; wherein Ci _-8 alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, amino, C _1-6 alkanylamino, di(Ci- ₆ alkanyl)amino, C-i-βalkanyloxy, thioC-i-βalkanyloxy, hydroxy, fluoro, chloro, cyano, aminocarbonyl, Ci _-8 alkanylaminocarbonyl, di(Ci_ ₈ alkanyl)aminocarbonyl, C-|. ₆ alkanyloxycarbonyl, and aryloxy; and wherein any aryl-containing substituents and Ci _-8 cycloalkanyl substituents of R ₂ are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, C _2-8 alkenyl, C _2-8 alkynyl, C-ι _-8 alkanyloxy, trifluoromethyl, trifluoromethoxy, phenyl, halogen, cyano, hydroxy, C-i-salkanylthio, Ci- ₈ alkanylsulfonyl, and Ci _-8 alkanylsulfonylamino; or R ₁ and R ₂ taken together with the nitrogen to which they are attached form a 5-7 membered cycloheteroalkyl optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, hydroxy(Ci _-8 )alkanyl, hydroxy, amino, C-i-βalkanylamino, di(Ci_ ₆ alkanyl)amino , and halogen;

R ₃ is a substituent selected from the group consisting of hydrogen, Ci _-8 alkanyl, halθi _-3 (Ci _-8 )alkanyl, C _2-8 alkenyl, C _2-8 alkynyl, C _3-8 cycloalkanyl, cycloalkanyl(Ci _-8 )alkanyl, C _1-8 alkanyloxy(C-i _-8 )alkanyl, Ci.8alkanylthio(Ci _-8 )alkanyl, hydroxyCi- ₈ alkanyl, Ci _-8 alkanyloxycarbonyl, haloi- ₃ (Ci _-8 )alkanylcarbonyl, formyl, thioformyl, carbamimidoyl, phenylimino(Ci- ₈ )alkanyl, phenyl(Ci _-8 )alkanyl, phenyl(C _1-8 )alkenyl, phenyl(Ci _-8 )alkynyl, naphthyl(Ci _-8 )alkanyl and heteroaryl(C _1-8 )alkanyl wherein the heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, thienyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl,

005/046691

pyrazolyl, pyridazinyl, pyrimidinyl, pyrrolyl, quinolinyl, isoquinolinyl, tetrazolyl, thiazolyl; wherein phenyl, naphthyl and heteroaryl are optionally substituted with one to three substituents independently selected from the group consisting of Ci- ₆ alkanyl, C- ₂ - ₆ alkenyl, Ci _-6 alkanyloxy, amino, Ci _-6 alkanylannino, di(Ci- ₆ alkanyl)amino, Ci _-6 alkanylcarbonyl, C-i-βalkanylcarbonyloxy, C-i-ealkanylcarbonylamino, C-i-βalkanylthio, Ci _-6 alkanylsulfonyl, halogen, hydroxy, cyano, fluoro(Ci_ ₆ )alkanyl, thioureido, and fluoro(Ci _-6 )alkanyloxy; alternatively, when phenyl and heteroaryl are optionally substituted with alkanyl or alkanloxy substituents attached to adjacent carbon atoms, the two substituents can together form a fused cyclic alkanyl or cycloheteroalkanyl selected from the group consisting of -(CH2)3-5- , -O(CH ₂ )2-4- , - (CHz) _2-4 O -, and -O(CH ₂ ) _1-3 O-;

R ₄ is one to three substituents independently selected from the group consisting of hydrogen; C _2-6 alkenyl; C _2-6 alkynyl; aryl(C _2-6 )alkynyl; Ci _-6 alkanyloxy; amino; C-i-βalkanylamino; di(Ci- ₆ alkanyl)amino; C _6- ioarylamino wherein Cβ-ioaryl is optionally substituted with one to three substitutents independently selected from the group consisting of C-i-βalkanyl, halogen, and hydroxy; formylamino; pyridinylamino; C-i-βalkanylcarbonyl; Ci-βalkanylcarbonyloxy; Ci- ₆ alkanyloxycarbonyl; aminocarbonyl; Ci-ealkanylaminocarbonyl; di(Ci _-6 alkanyl)aminocarbonyl; Ci _-6 alkanylcarbonylamino; Ci-βalkanylthio; C-i-βalkanylsulfonyi; halogen; hydroxy; cyano; hydroxycarbonyl; C _6- ioaryl; chromanyl; chromenyl; furanyl; imidazolyl; indazolyl; indolyl; indolinyl; isoindolinyl; isoquinolinyl; isothiazolyl; isoxazolyl; naphthyridinyl; oxazolyl; pyrazinyl; pyrazolyl; pyridazinyl; pyridinyl; pyrimidinyl; pyrrolyl; quinazolinyl; quinolinyl; quinolizinyl; quinoxalinyl; tetrazolyl; thiazolyl; thienyl; fluoroalkanyl and fluoroalkanyloxy; or optionally; when R ₄ is two substituents attached to adjacent

carbon atoms; the two substituents together form a single fused moiety; wherein the fused moiety is -(CH ₂ )3-5- , -O(CH ₂ )2-4- , -(CHa) _2-4 O-, -0(CH ₂ ) _I-3 O-, or -S-C(NH ₂ )=N-;

R ₅ is one to two substituents independently selected from the group consisting of hydrogen, d-βalkanyl, C ₂ - ₆ alkenyl, Ci _-6 alkanyloxy, amino, d-βalkanylamino, di(Ci- ₆ alkanyl)amino, d-βalkanylcarbonyl, d-βalkanylcarbonyloxy, Ci _-6 alkanyloxycarbonyl, d-βalkanylaminocarbonyl, d-βalkanylcarbonylamino, C-i-βalkanylthio, Ci _-6 alkanylsulfonyl, halogen, hydroxy, cyano, fluoro(d-6)alkanyl and fluoro(d- ₆ )alkanyloxy;

A is -(CH ₂ ) _m -, wherein m is 2 or 3;

Y is O or S;

Z is O, S, NH, N(C _1-6 alkanyl), N(OH), N(OC _1-6 alkanyl), or N(phenyl); and enantiomers, diastereomers, tautomers, solvates, or pharmaceutically acceptable salts thereof.

Embodiments of the present invention include compounds of Formula (I) wherein, preferably: a) G is -C(Z)N(Ri)R ₂ , phenyl, or a heterocycle selected from the group consisting of imidazolyl, triazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, imidazolinyl, tetrahydropyrimidinyl, thienyl, pyrazolyl, pyrimidinyl, triazinyl, isothiazolyl, isoxazolyl, oxazolyl, isoxadiazolyl, and pyridinyl; wherein phenyl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of d-βalkanyl, d-salkanyloxy, hydroxy(d-8)alkanyl, carboxy(d-8)alkanyl, Ci-salkanylcarbonylamino, halogen, hydroxy, cyano, oxo, thioxo, amino, Ci _-6 alkanylamino, di(Ci _-6 alkanyl)amino, d-salkanylthio, aminocarbonyl, aminothiocarbonyl, Ci_ ₈ alkanylaminocarbonyl, di(Ci- ₈ alkanyl)aminocarbonyl, and d-βalkanyloxycarbonylamino;

b) G is -C(Z)N(Ri)R ₂ , phenyl, or a heterocycle selected from the group consisting of imidazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, imidazolinyl, thienyl, pyrazolyl, pyrimidinyl, triazinyl, isothiazolyl, isoxazolyl, oxazolyl, isoxadiazolyl, and pyridinyl; wherein phenyl and the heterocycles of G (described herein) are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-4 alkanyl, hydroxy(Ci _-4 )alkanyl, carboxy(Ci- ₄ )alkanyl, Ci^alkanylcarbonylamino, hydroxy, cyano, oxo, thioxo, amino, C _1-6 alkanylamino, di(Ci_ ₆ alkanyl)amino, Ci _-8 alkanylthio, aminocarbonyl, aminothiocarbonyl, C-i-salkanylaminocarbonyl, and di(Ci- ₈ alkanyl)aminocarbonyl; c) G is -C(Z)N(Ri)R ₂ , phenyl, or a heterocycle selected from the group consisting of imidazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, thienyl, isothiazolyl, isoxazolyl, isoxadiazolyl, and pyridinyl; wherein phenyl and the heterocycles of G (described herein) are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-4 alkanyloxy, hydroxy(C _1-4 )alkanyl, hydroxy, cyano, oxo, thioxo, and aminocarbonyl; d) Ri is a substituent selected from the group consisting of hydrogen and Ci _-4 alkanyl; e) R ₁ is selected from the group consisting of hydrogen, methyl, ethyl, and propyl; f) Ri is selected from the group consisting of hydrogen, methyl, or ethyl; g) R ₂ is selected from the group consisting of hydrogen; C _1-4 alkanyl; phenyl; and Ci-βcycloalkanyl; wherein Ci _-4 alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, amino, Ci-βalkanylamino, di(Ci- ₆ alkanyl)amino, Ci _-4 alkanyloxy, hydroxy, fluoro, chloro, cyano, aminocarbonyl, C-i-salkanylaminocarbonyl, di(C-ι- ₈ alkanyl)aminocarbonyl, and phenoxy;

and wherein any phenyl-containing substituents and C-i-βcycloalkanyl substituents of R ₂ are optionally substituted with one to three substituents independently selected from the group consisting of C-i-salkanyl, Ci _-8 alkanyloxy, trifluoromethyl, phenyl, fluoro, hydroxy, C _1-8 alkanylthio, Ci- ₈ alkanylsulfonyl, and Ci- ₈ alkanylsulfonylamino; or Ri and R ₂ taken together with the nitrogen to which they are attached form a 5-7 membered cycloheteroalkyl optionally substituted with one to three substituents independently selected from the group consisting of Ci- ₄ alkanyl, hydroxy(Ci- ₄ )alkanyl, hydroxy, amino, Ci _-6 alkanylamino, di(C-i- ₆ alkanyl)amino, and fluoro; h) R ₂ is selected from the group consisting of hydrogen, phenyl, and Ci _-6 cycloalkanyl, wherein Ci _-4 alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, C- _M alkanyloxy, hydroxy, fluoro, aminocarbonyl, Ci _-8 alkanylaminocarbonyl, di(Ci _-8 alkanyl)aminocarbonyl, and phenoxy; and wherein any phenyl-containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of Ci-βalkanyl, Ci _-6 alkanyloxy, fluoro, hydroxy, and Ci _-6 alkanylthio; or Ri and R ₂ taken together with the nitrogen to which they are attached form a 5-7 membered cycloheteroalkyl optionally substituted with one to three substituents independently selected from the group consisting of Ci- ₄ alkanyl and hydroxy; i) R ₂ is selected from the group consisting of hydrogen, Ci- ₄ alkanyl and phenyl, wherein Ci_ ₄ alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, Ci _-4 alkanyloxy, hydroxy, fluoro, and phenoxy; and wherein any phenyl- containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of Ci _-6 alkanyl, Ci _-6 alkanyloxy, fluoro, and hydroxy; or Ri and R ₂ taken together with the nitrogen to which they are attached form a pyrrolidinyl or piperidinyl ring wherein said pyrrolidinyl or piperidinyl is optionally

substituted with a substituent selected from the group consisting of C _{1- 3} alkanyl and hydroxy; j) R3 is selected from the group consisting of hydrogen, d-aalkanyl, C ₂ - ₈ alkenyl, C _2-8 alkynyl, Ci _-8 alkanyloxy(Ci- ₈ )alkanyl, Ci. ₈ alkanylthio(Ci- ₈ )alkanyl, hydroxyCi-salkanyl, thioformyl, phenylimino(Ci- ₈ )alkanyl, phenyl(Ci _-8 )alkanyl, and heteroaryl(Ci _-8 )alkanyl wherein heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, thienyl, indolyl, indolinyl, isoquinolinyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, pyrrolyl, quinolinyl, isoquinolinyl, tetrazolyl; wherein phenyl and heteroaryl are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-6 alkanyloxy and hydroxy; or optionally, when phenyl and heteroaryl are optionally substituted with two substituents attached to adjacent carbon atoms, the two substituents together form a single fused moiety; wherein the moiety is selected from -O(CH ₂ ) _1-3 O-; k) R ₃ is selected from the group consisting of hydrogen, methyl, allyl, 2-methyl-allyl, propynyl, hydroxyethyl, methylthioethyl, methoxyethyl, thioformyl, phenyliminomethyl, phenethyl, and heteroaryl(Ci_ ₈ )alkanyl wherein the heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, thienyl, pyrimidinyl, pyrrolyl, quinolinyl, isoquinolinyl, tetrazolyl; wherein the phenyl in any phenyl-containing substituent is optionally substituted with one hydroxyl group;

I) R ₃ is hydrogen, methyl, allyl, or heteroarylmethyl wherein heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, and thienyl; m) R ₄ is one to three substituents independently selected from the group consisting of hydrogen; Ci-βalkanyl; Ci _-6 alkanyloxy; Cβ-ioarylamino wherein C _6- ioaryl is optionally substituted with one to three substitutents independently selected from the group consisting of Ci _-6 alkanyl;

Ci _-6 alkoxy, halogen, and hydroxy; formylamino; pyridinylamino; aminocarbonyl; Ci-βalkanylaminocarbonyl; Ci _-6 alkanylcarbonylamino; halogen; hydroxy; C _6- ioaryl; chromanyl; chromenyl; furanyl; imidazolyl; indazolyl; indolyl; indolinyl; isoindolinyl; isoquinolinyl; isothiazolyl; isoxazolyl; naphthyridinyl; oxazolyl; pyrazinyl; pyrazolyl; pyridazinyl; pyridinyl; pyrimidinyl; pyrrolyl; quinazolinyl; quinolinyl; quinolizinyl; quinoxalinyl; tetrazolyl; thiazolyl; and thienyl; n) R ₄ is one to two substituents independently selected from the group consisting of hydrogen, Ci _-4 alkanyl, Ci _-4 alkanyloxy, halogen, phenyl, furanyl, imidazolyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridinyl, pyrimidinyl, pyrrolyl, quinolinyl, tetrazolyl, thiazolyl, thienyl, and hydroxy; o) R ₄ is one to two substituents independently selected from the group consisting of hydrogen, methyl, methoxy, bromo, fluoro, α ¹ - or β ¹ - phenyl, α'- or β'~ pyridinyl, α ¹ - or β ¹ - furanyl, and hydroxy; p) R ₅ is one to two substituents independently selected from the group consisting of hydrogen and halogen; q) R ₅ is hydrogen; r) A is -(CHz) _2-3 -; s) A is -(CH ₂ ) ₂ -; t) Y is O or S; u) Z is O, NH, N(C _1-6 alkanyl), N(OH), N(OC _1-6 alkanyl), or N(phenyl); v) Z is O, NH, or N(OH); w) Z is O or NH; aa) G is -C(Z)N(R ₁ )R ₂ , phenyl, or a heterocycle selected from the group consisting of tetrazolyl, oxadizolyl, furyl, quinolinyl, thienyl, and pyridinyl; wherein phenyl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, Ci- ₈ alkanyloxy, hydroxy(Ci _-8 )aikanyl, carboxy(Ci _-8 )alkanyl,

Ci-salkanylcarbonylamino, halogen, hydroxy, cyano, oxo, thioxo, amino, Ci _-6 alkanylamino, di(Ci _-6 alkanyl)amino, C _1-8 alkanylthio, aminocarbonyl, aminothiocarbonyl, C _1-8 alkanylaminocarbonyl, di(Ci- ₈ alkanyl)aminocarbonyl, and Ci-ealkanyloxycarbonylamino; bb) G is -C(Z)N(Ri )R2, phenyl, or a heterocycle selected from the group consisting of tetrazolyl, oxadizolyl, furyl, quinolinyl, thienyl, and pyridinyl; wherein phenyl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of C- _M alkanyl, Ci^alkanyloxy, hydroxy(Ci- ₄ )alkanyl, Ci _-4 alkanylcarbonylamino, hydroxy, cyano, oxo, thioxo, and aminocarbonyl; cc) G is -C(Z)N(Ri)R ₂ ; tetrazolyl; pyridinyl; oxadiazolyl optionally substituted with oxo; or phenyl optionally substituted with (Ci _-8 )alkanylcarbonylamino; dd) G Js-C(Z)N(R ₁ )R ₂ , I H-tetrazol-4-yl, 4H-[1 ,2,4]-oxadiazol-5-oxo-3-yl, 2- methylcarbonylaminophenyl, pyridin-3-yl or pyridin-3-yl; dd) R ₂ is selected from the group consisting of hydrogen and Ci^alkanyl; wherein Ci^alkanyl is optionally substituted with phenyl; or R ₁ and R ₂ taken together with the nitrogen to which they are attached form a pyrrolidinyl wherein said pyrrolidinyl is optionally substituted with hydroxy; ee) R ₂ is selected from the group consisting of hydrogen, methyl, ethyl, and phenethyl; or Ri and R ₂ taken together with the nitrogen to which they are attached form pyrrolidin-1-yl, 3-hydoxypyrrolidin-1-yl or 3-(S)- hydoxypyrrolidin-1 -yl; ff) R ₃ is selected from the group consisting of hydrogen, C-i-salkanyl, C- ₂ - ₈ alkeny], C _2-8 alkynyl, C _1-8 alkanyloxy(Ci _-8 )alkanyl, Ci _-8 alkanylthio(Ci _-8 )alkanyl, hydroxyCi-salkanyl, thioformyl, phenylimino(Ci-s)alkanyl, phenyl(Ci _-8 )alkanyl, and heteroaryl(Ci- ₈ )alkanyl wherein heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, thienyl, indolyl, indolinyl, isoquinolinyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, pyrrolyl, quinolinyl, thiazolyl, isoquinolinyl, tetrazolyl; wherein phenyl and heteroaryl are optionally substituted with one to three substituents independently

selected from the group consisting of C _1-6 alkanyloxy and hydroxy; or optionally, when phenyl and heteroaryl are optionally substituted with two substituents attached to adjacent carbon atoms, the two substituents together form a single fused moiety; wherein the moiety is selected from -O(CH ₂ ) _1-3 O-; gg) R ₃ is selected from the group consisting of hydrogen, methyl, methylbutenyl, propenyl, benzyl, phenethyl, and heteroaryl(Ci _-8 )alkanyl wherein the heteroaryl is selected from the group consisting of imidazolyl, furanyl, pyridinyl, thienyl, and thiazolyl; hh) R ₃ is selected from the group consisting of hydrogen, methyl, 3-methyl- 2-butenyl, 2-propenyl, benzyl, 2-phenethyl, pyridin-2-ylmethyl , fur-3- ylmethyl, thiophene-2-ylmethyl, 1 H-imidazol-2-ylmethyl, and thiazol-2- ylmethyl; ii) R ₄ is one to two substituents independently selected from the group consisting of hydrogen, methyl, phenyl, bromo, fluoro, aminocarbonyl, chloro and hydroxy; jj) R ₄ is one to two substituents independently selected from the group consisting of hydrogen, α'-hydroxy and α'-methoxy; kk) R ₄ is unsubstituted or substituted at the α' position; II) R ₄ is hydrogen and Y is O; mm) R ₄ is α'-hydroxy and Y is O; nn) R ₄ is hydrogen and Y is S; 00) R ₄ is α'-hydroxy and Y is S; and combinations of a) through 00) above.

One embodiment of the present invention is a compound of Formula (I) wherein:

G is -C(Z)N(Ri)R ₂ , phenyl, or a heterocycle selected from the group consisting of imidazolyl, triazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, imidazolinyl, tetrahydropyrimidinyl, thienyl, pyrazolyl, pyrimidinyl, triazinyl, isothiazolyl, isoxazolyl, oxazolyl, isoxadiazolyl, and

pyridinyl; wherein phenyl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, Ci _-8 alkanyloxy, hydroxy(Ci _-8 )alkanyl, carboxy(Ci _-8 )alkanyl, C^salkanylcarbonylamino, halogen, hydroxy, cyano, oxo, thioxo, amino, C-i-βalkanylamino, di(Ci _-6 alkanyl)amino, Ci _-8 alkanylthio, aminocarbonyl, aminothiocarbonyl, Ci-salkanylaminocarbonyl, di(Ci_ ₈ alkanyl)aminocarbonyl, and Ci-βalkanyloxycarbonylamino;

Ri is hydrogen or C _1-4 alkanyl;

R ₂ is selected from the group consisting of hydrogen; Ci _-4 alkanyl; phenyl; and Ci _-6 cycloalkanyl; wherein Ci _-4 alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, amino, Ci-βalkanylamino, di(C _1-6 alkanyl)amino, Ci- ₄ alkanyloxy, hydroxy, fluoro, chloro, cyano, aminocarbonyl, Ci _-8 alkanylaminocarbonyl, di(C-i _-8 alkanyl)aminocarbonyl, and phenoxy; and wherein the phenyl and C-i-βcycloalkanyl substituents of R ₂ are optionally substituted with one to three substituents independently selected from the group consisting of Ci _-8 alkanyl, Ci _-8 alkanyloxy, trifluoromethyl, phenyl, fluoro, hydroxy, Ci _-8 alkanylthio, Ci _-8 alkanylsulfonyl, and Ci _-8 alkanylsulfonylamino; or Ri and R ₂ taken together with the nitrogen to which they are attached form a 5-7 membered cycloheteroalkyl optionally substituted with one to three substituents independently selected from the group consisting of Ci- ₄ alkanyl, hydroxy(Ci- ₄ )alkanyl, hydroxy, amino, Ci _-6 alkanylamino, di(Ci- ₆ alkanyl)amino, and fluoro;

R ₃ is selected from the group consisting of hydrogen, Ci _-8 alkanyl, C ₂ - ₈ alkenyl, C _2-8 alkynyl, Ci _-8 alkanyloxy(Ci _-8 )alkanyl, Ci _-8 alkanylthio(Ci _-8 )alkanyl, hydroxyCi _-8 alkanyl, thioformyl, phenylimino(Ci _-8 )alkanyl, phenyl(Ci_ ₈ )alkanyl, and heteroaryl(Ci- ₈ )alkanyl wherein heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, thienyl, indolyl, indolinyl,

isoquinolinyl, pyrazinyl, pyrazolyl, pyridazinyl, pyrimidinyl, pyrrolyl, quinolinyl, isoquinolinyl, tetrazolyl; wherein phenyl and heteroaryl are optionally substituted with one to three substituents independently selected from the group consisting of Ci- ₆ alkanyloxy and hydroxy; or optionally, when phenyl and heteroaryl are optionally substituted with two substituents attached to adjacent carbon atoms, the two substituents together form a single fused moiety; wherein the moiety is selected from -O(CH ₂ ) _1-3 O- _;

R ₄ is one to three substituents independently selected from the group consisting of hydrogen; d-βalkanyl; d-βalkanyloxy; C _6- ioarylamino wherein C _6-10 aryl is optionally substituted with one to three substitutents independently selected from the group consisting of Ci- ₆ alkanyl; Ci _-6 alkoxy, halogen, and hydroxy; formylamino; pyridinylamino; aminocarbonyl; Ci- ₆ alkanylaminocarbonyl; Ci-βalkanylcarbonylamino; halogen; hydroxy; C _6- ioaryl; chromanyl; chromenyl; furanyl; imidazolyl; indazolyl; indolyl; indolinyl; isoindolinyl; isoquinolinyl; isothiazolyl; isoxazolyl; naphthyridinyl; oxazolyl; pyrazinyl; pyrazolyl; pyridazinyl; pyridinyl; pyrimidinyl; pyrrolyl; quinazolinyl; quinolinyl; quinolizinyl; quinoxalinyl; tetrazolyl; thiazolyl; and thienyl;

R ₅ is one to two substituents independently selected from the group consisting of hydrogen and halogen;

A is CH ₂ CH ₂ ;

Y is O or S;

Z is O, NH, N(Ci _-6 alkanyl), N(OH), N(OC _1-6 alkanyl), or N(phenyl); and enantiomers, diastereomers, tautomers, solvates, and pharmaceutically acceptable salts thereof.

Another embodiment of the present invention is a compound of Formula herein:

G is -C(Z)N(Ri)R ₂ , phenyl, or a heterocycle selected from the group consisting of imidazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl,

oxathiadiazolyl, imidazolinyl, thienyl, pyrazolyl, pyrimidinyl, triazinyl, isothiazolyl, isoxazolyl, oxazolyl, isoxadiazolyl, and pyridinyl; wherein phenyl and the heterocycles of G (described herein) are optionally substituted with one to three substituents independently selected from the group consisting of C-i _-4 alkanyl, Ci _-4 alkanyloxy, hydroxy(Ci _-4 )alkanyl, carboxy(Ci _-4 )alkanyl, Ci _-4 alkanylcarbonylamino, hydroxy, cyano, oxo, thioxo, amino, C-i _-6 alkanylamino, di(Ci- ₆ alkanyl)amino, Ci _-8 alkanylthio, aminocarbonyl, aminothiocarbonyl, C-i-salkanylaminocarbonyl, and di(Ci- ₈ alkanyl)aminocarbonyl;

Ri is selected from the group consisting of hydrogen, methyl, ethyl, and propyl;

R ₂ is selected from the group consisting of hydrogen, Ci _-4 alkanyl, phenyl, and Ci _-6 cycloalkanyl; wherein Ci _-4 alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, hydroxy, fluoro, aminocarbonyl, Ci _-8 alkanylaminocarbonyl, di(Ci _-8 alkanyl)aminocarbonyl, and phenoxy; and wherein any phenyl-containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of C-i-βalkanyl, C-i-βalkanyloxy, fluoro, hydroxy, and C-i-βalkanylthio; or Ri and Ra taken together with the nitrogen to which they are attached form a pyrrolidinyl or piperidinyl ring wherein said pyrrolidinyl or piperidinyl is optionally substituted with a substituent selected from the group consisting of Ci _-3 alkanyl and hydroxy;

R ₃ is selected from the group consisting of hydrogen, methyl, allyl, 2-methyl-allyl, propynyl, hydroxyethyl, methylthioethyl, methoxyethyl, thioformyl, phenyliminomethyl, phenethyl, and heteroaryl(Ci _-8 )alkanyl wherein the heteroaryl is selected from the group consisting of benzo[1 ,3]dioxolyl, imidazolyl, furanyl, pyridinyl, thienyl, pyrimidinyl, pyrrolyl, quinolinyl, isoquinolinyl, tetrazolyl wherein the phenyl in any phenyl-containing substituent is optionally substituted with one hydroxyl group;

R ₄ is one to two substituents independently selected from the group consisting of hydrogen, Ci _-4 alkanyl, halogen, phenyl, furanyl, imidazolyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridinyl, pyrimidinyl, pyrrolyl, quinolinyl, tetrazolyl, thiazolyl, thienyl, and hydroxy;

R ₅ is hydrogen;

A is CH ₂ CH ₂ ;

Y is O or S;

Z is O, NH, or N(OH); and enantiomers, diastereomers, tautomers, solvates, and pharmaceutically acceptable salts thereof.

Another embodiment of the present invention is directed to compositions comprising a compound of Formula (I) wherein:

G is selected from -C(Z)N(Ri)R ₂ , phenyl, or a heterocycle selected from the group consisting of imidazolyl, tetrazolyl, oxadiazolyl, thiadiazolyl, oxathiadiazolyl, thienyl, isothiazolyl, isoxazolyl, isoxadiazolyl, and pyridinyl; wherein phenyl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of C _1-4 alkanyloxy, hydroxy(Ci-4)alkanyl, Ci- ₄ alkanylcarbonylamino, hydroxy, cyano, oxo, thioxo, and aminocarbonyl;

Ri is hydrogen, methyl, or ethyl;

R ₂ is independently selected from the group consisting of hydrogen,

C- _M alkanyl and phenyl; wherein is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, C _1-4 alkanyloxy, hydroxy, fluoro, and phenoxy; and wherein any phenyl-containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of Ci _-6 alkanyl, d-βalkanyloxy, fluoro, and hydroxy; or Ri and

R2 taken together with the nitrogen to which they are attached form a pyrrolidinyl or piperidinyl ring wherein said pyrrolidinyl or piperidinyl are optionally substituted with a substituent selected from the group consisting of Ci- ₃ alkanyl and hydroxy;

R ₃ is hydrogen, methyl, allyl, or heteroarylmethyl wherein heteroaryl is selected from the group consisting of benzo[1,3]dioxolyl, imidazolyl, furanyl, pyridinyl, and thienyl;

R ₄ is one to two substituents independently selected from the group consisting of hydrogen, Ci _-4 alkanyl, Ci _-4 alkanyloxy, halogen, phenyl, furanyl, imidazolyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridinyl, pyrimidinyl, pyrrolyl, quinolinyl, tetrazolyl, thiazolyl, thienyl, and hydroxy;

A is CH ₂ CH ₂ ;

Y is O or S;

Z is O or NH; and enantiomers, diasteromers, tautomers, solvates, and pharmaceutically acceptable salts thereof.

Another embodiment of the present invention is directed to compounds ormula (I) wherein:

G is -C(Z)N(Ri)R ₂ , phenyl, or a heterocycle selected from the group consisting of tetrazolyl, oxadiazolyl, furyl, quinolinyl, thienyl, and pyridinyl; wherein phenyl and the heterocycles of G are optionally substituted with one to three substituents independently selected from the group consisting of C-t-salkanyl, C-i-βalkanyloxy, hydroxy(Ci _-8 )alkanyl, carboxy(Ci- ₈ )alkanyl, C-i-salkanylcarbonylamino, halogen, hydroxy, cyano, oxo, thioxo, amino, Ci _-6 alkanylamino, di(Ci-6alkanyl)amino, Ci _-8 alkanylthio, aminocarbonyl, aminothiocarbonyl, C-i-aalkanylaminocarbonyl, di(Ci- ₈ alkanyl)aminocarbonyl, and Ci-βalkanyloxycarbonylamino;

R ₁ is C _1-4 alkanyl, or hydrogen;

R ₂ is hydrogen or C _1-4 alkanyl optionally substituted with phenyl; or Ri and R ₂ taken together with the nitrogen to which they are attached form a pyrrolidinyl ring optionally substituted with hydroxy; Z is NH or oxygen; R ₃ is pyridinyl(C _1-8 )alkanyl, furyl(C-i- ₈ )alkanyl, C _1-8 alkanyl, hydrogen, C _2-8 alkenyl, thienyl(C _1-8 )alkanyl, imidazolyl(C _1-8 )alkanyl, phenyl(C _1-8 )alkanyl, or thiazolyl(C _1-8 )alkanyl; R ₄ is hydrogen, C _1-B alkanyl, C _1-6 alkanyloxy, hydroxy, halogen, aminocarbonyl, or phenyl; R ₅ is hydrogen; A is CH ₂ CH ₂ ; Y is O or S; Z is O or NH; and enantiomers, diastereomers, tautomers, solvates, and pharmaceutically acceptable salts thereof.

Another embodiment of the present invention is directed to compounds ormula (I) wherein:

G is -C(Z)N(R ₁ )R ₂ ; tetrazolyl, oxadiazolyl optionally substituted with oxo; phenyl optionally substituted with (C-|. ₈ )alkanylcarbonylamino; or pyridinyl;

R ₁ is C-ι- ₄ alkanyl, or hydrogen;

R ₂ is hydrogen or C _1-4 alkanyl optionally substituted with phenyl; or R ₁ and R ₂ taken together with the nitrogen to which they are attached form a pyrrolidinyl ring optionally substituted with hydroxy; Z is NH or oxygen; R ₃ is pyridinyl(C _1-8 )alkanyl, furyl(C _1-8 )alkanyl, Ci _-8 alkanyl, hydrogen, C _2-8 alkenyl, thienyl(C _1-8 )alkanyl, imidazolyl(Ci _-8 )alkanyl, phenyl(C _1-8 )alkanyl, or thiazolyl(C _1-8 )alkanyl; R ₄ is hydrogen, α'-hydroxy, or α'-methoxy;

46691

R ₅ is hydrogen; A is CH ₂ CH ₂ ;

Y is O or S;

Z is O or NH; and enantiomers, diastereomers, tautomers, solvates, and pharmaceutically acceptable salts thereof.

Another embodiment of the present invention is directed to compounds ormula (I) wherein:

G is -C(Z)N(Ri)R ₂ , 1 H-tetrazol-4-yl, 4H-[1 ,2,4]-oxadiazol-5-oxo-3-yl, 2- methylcarbonylaminophenyl, 3-furyl, quinolin-3-yl, thiophen-3-yl, pyridin-

3-yl or pyridin-3-yl, Ri is hydrogen, ethyl, or methyl, R ₂ is methyl, ethyl, phenethyl, or hydrogen; or Ri and R ₂ taken together with the nitrogen to which they are attached form pyrrolidin-1-yl, 3-hydroxypyrrolidin-1-yl, or 3-(S)-hydroxypyrrolidin-

1-yi;

Z is NH or oxygen,

R ₃ is pyridin-2-ylmethyl , fur-3-ylmethyl, methyl, hydrogen, 3-methyl-2- butenyl, thiophene-2-ylmethyl, 2-propenyl, 1H-imidazol-2-ylmethyl, 2- phenethyl, thiazol-2-ylmethyl, benzyl, or allyl; R ₄ is hydrogen, α'-methyl, α'-phenyl, β"-bromo, β"-fluoro, α'-aminocarbonyl, α'-chloro, α'-methoxy; or α'-hydroxy; R ₅ is hydrogen; A is CH ₂ CH ₂ ;

Y is O or S;

Z is O or NH; and enantiomers, diastereomers, tautomers, solvates, and pharmaceutically acceptable salts thereof

Another embodiment of the present invention is directed to compounds

of Formula (I) wherein:

G Js -C(Z)N(R ₁ )R ₂ , 1 H-tetrazol-4-yl, 4H-[1 ,2,4]-oxadiazol-5-oxo-3-yl, 2- methylcarbonylaminophenyl, pyridin-3-yl or pyridin-3-yl; Ri is hydrogen, ethyl, or methyl; R ₂ is methyl, ethyl, phenethyl, or hydrogen; or R ₁ and R ₂ taken together with the nitrogen to which they are attached form pyrrolidin-1-yl, 3-hydroxypyrrolidin-1-yl, or 3-(S)-hydroxypyrrolidin-

1-yi;

Z is NH or oxygen;

R ₃ is pyridin-2-ylmethyl, fur-3-ylmethyl, methyl, hydrogen, 3-methyl-2- butenyl, thiophene-2-ylmethyl, 2-propenyl, 1 H-imidazol-2-ylmethyl, 2- phenethyl, thiazol-2-ylmethyl, or benzyl; R ₄ is hydrogen, α'-hydroxy, or α'-methoxy; R ₅ is hydrogen; A is CH ₂ CH ₂ ; Y is O or S; Z is O or NH; and enantiomers, diastereomers, tautomers, solvates, and pharmaceutically acceptable salts thereof.

In certain embodiments of Formula (I) when R ₁ and R ₂ are taken together with the nitrogen to which they are attached to form a 5-7 membered cycloheteroalkyl optionally substituted with one to three substituents independently selected from the group consisting of C _1-8 alkanyl, hydroxy(C-|. β)alkanyl, hydroxy, amino, C-i-βalkanylamino, di(C _1-6 alkanyl)amino, and halogen; Z is oxygen.

In certain embodiments of Formula (I) when R ₁ and R ₂ are taken together with the nitrogen to which they are attached to form a pyrrolidinyl ring optionally substituted with hydroxy, Z is oxygen.

Another embodiment of the present invention is a compound of Formula>(I) wherein:

G is selected from -C(Z)N(Ri )R2, 2-methylcarbonylaminophenyl, 2-aminocarbonyl-phenyl, 1 H-tetrazol-4-yl, 2-methyl-tetrazol-5-yl, 4H-[1 ,2,4]-oxadiazol-5-oxo-3-yl, 4H-[1 ,2,4]-oxadiazol-5-thioxo-3-yl, 4H-[1 ,2,4]thiadiazol-5-oxo-3-yl, [1 ,2,3,5]oxathiadiazol-2-oxo-4-yl, or pyridin-3-yl;

Ri is hydrogen, methyl, or ethyl;

R ₂ is selected from the group consisting of hydrogen, Ci _-4 alkanyl and phenyl; wherein C- _M alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, C _1-4 alkanyloxy, hydroxy, fluoro, and phenoxy; and wherein any phenyl- containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of C-i-βalkanyl, d-βalkanyloxy, fluoro, and hydroxy; or Ri and R ₂ taken together with the nitrogen to which they are attached form a pyrrolidinyl or piperidinyl ring;

R ₃ is selected from the group consisting of hydrogen, Ci _-8 alkanyl, C _2-8 alkenyl, C _2-8 alkynyl, Ci _-8 alkanyloxy(Ci. ₈ )alkanyl ₎ Ci- ₈ alkanylthio(Ci- ₈ )alkanyl, hydroxyCi-salkanyl, thioformyl, phenylimino(Ci _-8 )alkanyl, phenyl(Ci- _δ )alkanyl, and heteroaryl(Ci- ₈ )alkanyl wherein heteroaryl is selected from the group consisting of hydrogen, methyl, allyl, or heteroarylmethyl; wherein heteroaryl is selected from the group consisting of benzo[1,3]dioxolyl, imidazolyl, furanyl, pyridinyl, and thienyl; wherein phenyl and heteroaryl are optionally substituted with one to three substituents independently selected from the group consisting of C-i-βalkanyloxy and hydroxy; or optionally, when phenyl and heteroaryl are optionally substituted with two substituents attached to adjacent carbon atoms, the two substituents together form a single fused moiety; wherein the moiety is selected from -O(CH2)i- ₃ θ-;

R ₄ is one to three substituents independently selected from the group consisting of hydrogen, Ci _-4 alkanyloxy, halogen, phenyl, furanyl, imidazolyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridinyl, pyrimidinyl, pyrrolyl, quinolinyl, tetrazolyl, thiazolyl, thienyl, and hydroxy;

Rs is hydrogen;

A is CH ₂ CH ₂ ;

Y is O or S;

Z is O or NH; and enantiomers, diastereomers, tautomers, solvates, and pharmaceutically acceptable salts thereof.

Another embodiment of the present invention is directed to compositions comprising a compound of Formula (I) wherein G is independently selected from -C(Z)N(R ₁ )R ₂ , 2-methylcarbonylaminophenyl, 2-aminocarbonyl-phenyl, 1H-tetrazol-4-yl, 2-methyl-tetrazol-5-yl, 4H-[1 ,2,4]-oxadiazol-5-oxo-3-yl, 4tf-[1 ,2,4]-oxadiazol-5-thioxo-3-yl, 4H-[1 ,2,4]thiadiazol-5-oxo-3-yl, [1 ,2,3,5]oxathiadiazol-2-oxo-4-yl, and pyridin-3-yl; Ri is hydrogen, methyl, or ethyl; R ₂ is a substituent selected from the group consisting of hydrogen, Ci _-4 alkanyl and phenyl; wherein Ci_ ₄ alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, Ci _-4 alkanyloxy, hydroxy, and 2,6-dimethyl-phenoxy; and wherein the any phenyl-containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of C _1-6 alkanyl, d-βalkanyloxy, fluoro, and hydroxy; or Ri and R ₂ taken together with the nitrogen to which they are attached form a pyrrolidinyl or piperidinyl ring wherein said pyrrolidinyl or piperidinyl is optionally substituted with a substituent selected from the group consisting of Ci _-3 alkanyl and hydroxy; R ₃ is a substituent selected from the group consisting of benzo[1 ,3]dioxol-5-ylmethyl, carbamimidoyl, 1-H-imidazol-2-ylmethyl, phenyliminomethyl, 1-prop-2-ynyl,

thioformyl, 2-hydroxyphenyl-methyl, hydroxy-ethyl, methoxy-ethyl, 2-methyl-allyl, 2-methyl-but-2-enyl, allyl, furan-3-ylmethyl, H, Me, methylthioethyl, phenethyl, pyridin-3-yl methyl, and thiophen-2-ylmethyl; R ₄ is one to two substituents independently selected from the group consisting of hydrogen, C _1-4 alkanyloxy, halogen, phenyl, furanyl, imidazolyl, indazolyl, indolyl, indolinyl, isoindolinyl, isoquinolinyl, isothiazolyl, isoxazolyl, oxazolyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridinyl, pyrimidinyl, pyrrolyl, quinolinyl, tetrazolyl, thiazolyl, thienyl, and hydroxy; Rs is H; A is CH ₂ CHb; Y is O or S; and Z is O or NH.

Another embodiment of the present invention is directed to compositions comprising a compound of Formula (I) wherein G is selected from - C(Z)N(Ri)R ₂ , 2-methylcarbonylaminophenyl, 2-aminocarbonyl-phenyl, 1H-tetrazol-4-yl, 2-methyl-tetrazol-5-yl, 4H-[1 ,2,4]-oxadiazol-5-oxo-3-yl, 4H-[1 ,2,4]-oxadiazol-5-thioxo-3-yl, 4H-[1 ,2,4]thiadiazol-5-oxo-3-yl, [1 ,2,3,5]oxathiadiazol-2-oxo-4-yl, or pyridin-3-yl; Ri is hydrogen, methyl, or ethyl; R ₂ is a substituent selected from the group consisting of hydrogen, Ci _-4 alkanyl and phenyl; wherein Ci- ₄ alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, methoxy, hydroxy, and 2,6-dimethyl-phenoxy; and wherein any phenyl- containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of C-i-βalkanyl, Ci _-6 alkanyloxy, fluoro, and hydroxy; or Ri and R ₂ taken together with the nitrogen to which they are attached form a pyrrolidinyl or piperidinyl ring wherein said pyrrolidinyl or piperidinyl are optionally substituted with a substituent selected from the group consisting of C-|. ₃ alkanyl and hydroxy; R ₃ is a substituent selected from the group consisting of benzo[1 ,3]dioxol-5-ylmethyl, carbamimidoyl, 1 -H-imidazol-4-yl methyl, phenyliminomethyl, 1-prop-2-ynyl, thioformyl, 2-hydroxyphenyl-methyl, hydroxyethyl, methoxyethyl, allyl, furan-3-yl methyl, H, Me, methylthioethyl, and phenethyl; R ₄ is one to two substituents independently selected from the group consisting of hydrogen, methyl,

46691

methoxy, bromo, fluoro, α ¹ - or β'-phenyl, α ¹ - or β'-pyridinyl, α'- or β'-furanyl, and hydroxy; R ₅ is H; A is CH ₂ CH ₂ ; Y is O or S; and Z is O or NH.

Another embodiment of the present invention is directed to compositions comprising a compound of Formula (I) wherein G is selected from - C(Z)N(Ri)R ₂ , 2-methylcarbonylaminophenyl, 2-aminocarbonyl-phenyl, 1 H-tetrazol-4-yl, 2-methyl-tetrazol-5-yl, 4H-[1 ,2,4]-oxadiazol-5-oxo-3-yl, 4H-[1 ,2,4]-oxadiazol-5-thioxo-3-yl, 4H-[1 ,2,4]thiadiazol-5-oxo~3-yl, [1,2,3,5]oxathiadiazol-2-oxo-4-yl, or pyridin-3-yl; Ri is hydrogen, methyl, or ethyl; R ₂ is a substituent selected from the group consisting of hydrogen, Ci _-4 alkanyl and phenyl; wherein Ci _-4 alkanyl is optionally substituted with one to three substituents independently selected from the group consisting of phenyl, methoxy, hydroxy, and 2,6-dimethyl-phenoxy; and wherein any phenyl- containing substituent of R ₂ is optionally substituted with one to three substituents independently selected from the group consisting of Ci _-6 alkanyl, C-i-βalkanyloxy, fluoro, and hydroxy; alternatively Ri and R ₂ are taken together with the nitrogen to which they are attached to form a pyrrolidinyl or piperidinyl ring wherein said pyrrolidinyl or piperidinyl are optionally substituted with a substituent selected from the group consisting of Ci- ₃ alkanyl and hydroxy; R ₃ is a substituent selected from the group consisting of H, benzo[1 ,3]dioxol-5-ylmethyl, 1 -H-imidazol-4-yl methyl, furan-3-ylmethyl, pyridin-2-ylmethyl , and phenyliminomethyl; R ₄ is a substituent independently selected from the group consisting of hydrogen, methyl, methoxy, bromo, fluoro cf- or β'-phenyl, α ¹ - or β'-pyridinyl, α'- or β'-furanyl, and hydroxy; R ₅ is H; A is CH ₂ CH ₂ ; Y is O or S; and Z is O or NH.

Another embodiment of the present invention is directed to a compound of Formula (I) wherein R ₄ is preferably substituted at the α ¹ - or β ¹ - position of Formula (I).

Another embodiment of the present invention is directed to compositions comprising a compound selected from the group consisting of: a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyI; R ³ is 1H- imidazol-2-yl-methyl; R ⁴ is α'-hydroxy; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is furan-

3-yl-methyl; R ⁴ is α'-hydroxy; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,/V-diethylaminocarbonyl; R ³ is H; R ⁴ is α'-hydroxy; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is α'-methoxy; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is pyridin-3-yl-methyl; R ⁴ is H; R ⁵ is H; Y is O; A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is furan-

3-yl-methyl; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is thien-

2-yl-methyl; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is benzyl; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is pyridin-3-yl; R ³ is furan-3-yl methyl; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is furan-

2-yl methyl; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is α'-methyl; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,/V-diethylaminocarbonyl; R ³ is H; R ⁴ is α'-phenyl; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -;

a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is β"-bromo; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is α'-chloro; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is H; R ⁴ is β"-fluoro; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is 2-methylcarbonylamino-phenyl; R ³ is

H; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is pyrrolidin-1-yl; R ³ is H; R ⁴ is H; R ⁵ is H;

Y is O; and A is -CH ₂ CH ₂ -; a compound of Formula (I) wherein G is Λ/,Λ/-diethylaminocarbonyl; R ³ is furan-

3-yl-methyl; R ⁴ is H; R ⁵ is H; Y is O; and A is -CH ₂ CH ₂ -;

Another embodiment of the present invention is directed to compounds and compositions comprising a compound selected from the group consisting of:

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin e-3-carboxylic acid diethylamide;

(3-Hydroxy-pyrrolidin-1-yl)-[10-(8-methyl-8-aza-bicyclo[3 .2.1]oct-3-yl)-10H- phenoxazin-3-yl)-methanone;

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin e-3-carboxylic acid methyl-phenethyl-amide;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H-phenoxazine; Endo-10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 3-(1 H-tetrazol-5-yl)- 10H-phenoxazine;

Endo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H- phenoxazine;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3- carbonitrile;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-N.N-diethyl-10H-pheno xazine-3- carboxamidine;

Endo-N.N-Diethyl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3 .2.1]oct-3-yl)-10H- phenoxazine-3-carboxamidine;

Endo-N.N-Diethyl-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H- phenoxazine-3-carboxamidine;

Endo- 3-[10-(3-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3-yl)-4H -

[1 ,2,4]oxadiazol-5-one; endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-car boxylic acid amide;

Endo-3-[10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazin-

3-yl)-4H-[1 ,2,4]oxadiazol-5-one;

Endo-3-[10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H -phenoxazin-3-yl)-

4H-[1 ,2,4]oxadiazol~5-one;

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin e-3-carboxylic acid diethylamide;

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-10H-phenoxazi ne-3-carboxylic acid diethylamide;

6-Methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 0H-phenoxazine-3- carboxylic acid diethylamide;

6-Hydroxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 0H-phenoxazine-3- carboxylic acid diethylamide;

6-Methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3- carboxylic acid diethylamide;

10-(8-Methyl-3-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H- phenoxazine;

N.N-Diethyl-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10 H-phenoxazine-3- carboxamidine;

3-[10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxa zin-3-yl)-4H-

[1 ,2,4]oxadiazol-5-one;

10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyrid in-3-yl-10H- phenothiazine;

N-{2-[10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl )-10H-phenothiazin-3- yl]-phenyl}-acetamide;

10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl) -3-pyridin-3-yl-10H- phenothiazine;

3-Pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2 .1]oct-3-yl)-10H- phenothiazine;

10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl) -3-pyridin-4-yl-10H- phenothiazine;

3-Pyridin-4-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2 .1]oct-3-yl)-10H- phenothiazine;

3-Pyridin-4-yl-10-(8-thiophen-2-ylmethyl-8-aza-bicyclo[3. 2.1]oct-3-yl)-10H- phenothiazine;

N-{2-[10-(8-Allyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno thiazin-3-yl)-phenyl}- acetamide;

N-{2-[10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-p henothiazin-3-yl)- phenyl}-acetamide;

N-(2-{10-[8-(1H-lmidazol-2-ylmethyl)-8-aza-bicyclo[3.2.1] oct-3-yl)-10H- phenothiazin-3-yl)-phenyl)-acetamide;

N-{2-[10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phen othiazin-3-yl)-phenyl}- acetamide;

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-phen othiazine; N-{2-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazin-3-y l)-phenyl}- acetamide;

10-(8-Allyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-phenothiazine; Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-ph enoxazine;

Endo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyri din-3-yl-10H- phenoxazine;

Endo-3-Pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicycl o[3.2.1]oct-3-yl)-10H- phenoxazine;

Endo-10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3- pyridin-3-yl-10H- phenoxazine;

Endo-3-Pyridin-3-yl-10-(8-thiophen-2-ylmethyl-8-aza-bicyc lo[3.2.1]oct-3-yl)-

10H-phenoxazine;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-chloro-10H-pheno xazine;

Endo-10^8-Aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-4-yl-10H- phθnoxazine;

Endo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyri din-4-yl-10H- phenoxazine;

Endo-3-Pyridin-4-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicycl o[3.2.1]oct-3-yl)-10H- phenoxazine;

Endo-10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl) -3-pyridin-3-yl-10H- phenoxazine;

Endo-3-Pyridin-4-yl-10-(8-thiophen-2-ylmethyl-8-aza-bicyc lo[3.2.1]oct-3-yl)-

10H-phenoxazine;

Exo-3-(3-Pyridin-3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2 .1]octane-8-carboxylic acid tert-butyl ester;

Exo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyrid in-3-yl-10H- phenoxazine;

Exo-3-Pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo [3.2.1]oct-3-yl)-10H- phenoxazine;

Exo-10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 3-pyridin-3-yl-10H- phenoxazine;

Exo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-chloro-10H-phenox azine;

Exo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H- phenoxazine;

Exo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyrid in-3-yl-10H- phenoxazine;

Exo-3-Pyridin-4-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo [3.2.1]oct-3-yl)-10H- phenoxazine;

Exo-10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 3-pyridin-3-yl-10H- phenoxazine;

Exo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H-phenoxazine;

Exo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carb onitrile;

Exo-3-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3 ~yl]-4H-

[1 ,2,4]oxadiazol-5-one;

6-Methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3- carbonitrile;

6-Hydroxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3- carbonitrile;

[ 10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phe nothiazin-3-yl)- pyrrolidin-1 -yl-methanone;

[ 10-(8-Phenθthyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothia zin-3-yl)-pyrrolidin-

1 -yl-methanone;

{10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl )-10H-phenothiazin-3- yl}-pyrrolidin-1 -yl-methanone;

[1 (H8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno thiazin-3-yl)-(3- hydroxy-pyrrolidin-1-yl)-methanone;

(3-Hydroxy-pyrrolidin-1-yl)-[10-(8-phenethyl-8-aza-bicycl o[3.2.1]oct-3-yl)-10H- phenothiazin-3-yl)-methanone;

{10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl )-10H-phenothiazin-3- yl}-(3-methyl-pyrrolidin-1-yl)-methanone;

[10-(3-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothia zin-3-yl)-pyrrolidin-1 - yl-methanone;

(3-Hydroxy-pyrrolidin-1 -yl)-[10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazin-3-yl)-methanone;

10-(3-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiaz ine-3-carboxylic acid ethylamide;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3- carboxylic acid diethylamide;

Endo-10^8-Thiophen^-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl )-10H- phenoxazine-3-carboxylic acid diethylamide;

Endo-10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-y l)-10H-phenoxazine-

3-carboxylic acid diethylamide;

Endo-10-(8-Thiazol-2-ylmethyl-8-aza-bicydo[3.2.1]oct-3-yl)-1 0H-phenoxazine-

3-carboxylic acid diethylamide;

Endo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-ph enoxazine-3- carboxylic acid diethylamide;

Endo-1 O^8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phe noxazine-

3-carboxylic acid diethylamide;

Endo-10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct- 3-yl)-1 OH- phenoxazine-3-carboxylic acid diethylamide;

Endo-10-[8-(1 H-lmidazol-2-ylmethyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-i OH- phenoxazine-3-carboxylic acid diethylamide;

Endo-10-(8-Benzyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno xazine-3-carboxylic acid diethylamide;

Exo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-c arboxylic acid diethylamide;

Exo-1 O^8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine- 3- carboxylic acid diethylamide;

Exo-10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl )-10H-phenoxazine-3- carboxylic acid diethylamide;

Exo-10-(8-Thiophen^-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl )-10H-phenoxazine-

3-carboxylic acid diethylamide;

Exo-10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3 -yl)-10H-phenoxazine-

3-carboxylic acid diethylamide;

Exo-10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl )-10H-phenoxazine-3- carboxylic acid diethylamide;

Exo-10-(8-Benzyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenox azine-3-carboxylic acid diethylamide;

Exo-1 O^8-Thiazol-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phe noxazine-3- carboxylic acid diethylamide;

Exo-10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3- carboxylic acid diethylamide;

Exo-10-[8-(1 H-lmidazol-2-ylmethyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3-carboxylic acid diethylamide;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-7-pyridin-3-yl-10H -phenoxazin-4-ol;

Endo-7-Pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicycl o[3.2.1]oct-3-yl)-10H- phenoxazin-4-ol;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-chloro-6-methoxy -10H-phenoxazine;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-3-pyridi n-3-yl-10H- phenoxazine;

Endo-8-Methoxy-3-pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8- aza-bicyclo[3.2.1]oct-

3-yl)-10H-phenoxazine;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-7-pyridin-^yl-10H- phenoxazin-4-ol;

Endo-7-Pyridin-4-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicycl o[3.2.1]oct-3-yl)-10H- phenoxazin-4-ol;

Endo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-7-pyri din-3-yl-10H- phenoxazin-4-ol;

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)- 6-methoxy-3-pyridin-3-yl-10H- phenoxazine;

Endo-6-Methoxy-3-pyridin-4-yl-10-(8-pyridin-2-ylmethyl-8- aza-bicyclo[3.2.1]oct-

3-yl)-10H-phenoxazine;

Endo-6-Methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3- yl)-3-pyridin-3-yl-

10H-phenoxazine;

Endo-N-{2-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxaz in-3-yl)-phenyl}- acetamide;

Endo-N-{2-[10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazin-3-yl)- phenyl}-acetamide;

Endo-N-{2-[10-(8-Thiophen-2-ylmethyl-8-aza-bicyclo[3.2.1] oct-3-yl)-10H- phenoxazin-3-yl)-phenyl}-acetamide;

Endo-N-{2-[10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]o ct-3-yl)-10H- phenoxazin-3-yl)-phenyl}-acetamide;

Exo-N-{2-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazi n-3-yl)-phenyl}- acetamide;

Endo-N-{2-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-6-hydroxy-10H-p henoxazin-3-yl)- phenyl}-acetamide;

Endo-N^-tiO^8-Aza-bicyclo[3.2.1]oct-3-yl)-8-methoxy-10H-p henoxazin-3-yl)- phenyl}-acetamide;

10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-CI H-tetrazol-8-yl)-10H- phenothiazine;

10-(8-Pyridin-2-ylmethyl-8-aza-bicyc!o[3.2.1]oct-3-yl)-3- (1 H-tetrazol-5-yl)-10H- phenothiazine;

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H-phenothiazine;

N.N-Diethyl-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl) -10H-phenothiazine-3- carboxamidine;

N.N-Diethyl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1] oct-3-yl)-10H- phenothiazine-3-carboxamidine;

10-(3-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H- phenothiazine;

N.N-Diethyl-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10 H-phenothiazine-3- carboxamidine;

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)- 6-methoxy-3-pyridin-3-yl-10H-phenothiazine;

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-7-pyridin-3-yl-10H-phθ nothiazin-4-ol;

6-Methoxy-10-(8-mθthyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H -phenothiazine-3- carboxylic acid diethylamide;

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-10H-phenothia zine-3-carboxylic acid diethylamide;

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-6-hydroxy-10H-phenothia zine-3-carboxylic acid diethylamide; and enantiomers, diastereomers, tautomers, solvates, or pharmaceutically acceptable salts thereof.

Another embodiment of the present invention is a composition comprising the dextrorotatory enantiomer of a compound of formula (I), wherein said composition is substantially free from the levorotatory isomer of said

4δ

compound. In the present context, substantially free means less than 25 %, preferably less than 10 %, more preferably less than 5 %, even more preferably less than 2 % and even more preferably less than 1 % of the levorotatory isomer calculated as.

Another embodiment of the present invention is a composition comprising the levorotatory enantiomer of a compound of formula (I) wherein said composition is substantially free from the dextrorotatory isomer of said compound. In the present context, substantially free from means less than 25 %, preferably less than 10 %, more preferably less than 5 %, even more preferably less than 2 % and even more preferably less than 1 % of the dextrorotatory isomer calculated as

In certain embodiments, the present invention provides the endo isomer of a compound of formula (I) wherein said compound is substantially free from the exo isomer of said compound. In certain embodiments, the present invention provides compositions comprising the endo isomer of a compound of formula (I) wherein said composition is substantially free from the exo isomer of said compound. In the present context, substantially free means less than 25 %, preferably less than 10 %, more preferably less than 5 %, even more preferably less than 2 % and even more preferably less than 1 % of the exo isomer.

In certain embodiments, the present invention provides the exo isomer of a compound of formula (I) wherein said compound is substantially free from the endo isomer of said compound. In certain embodiments, the present invention provides compositions comprising the exo isomer of a compound of formula (I) wherein said composition is substantially free from the endo isomer

of said compound. In the present context, substantially free means less than 25 %, preferably less than 10 %, more preferably less than 5 %, even more preferably less than 2 % and even more preferably less than 1 % of the endo isomer.

In other embodiments, compositions of the present invention comprise a mixture of the exo and endo isomers of a compound of formula (I).

The compounds of the present invention may also be present in the form of pharmaceutically acceptable salts. For use in medicine, the salts of the compounds of this invention refer to non-toxic "pharmaceutically acceptable salts" (Ref. International J. Pharm., 1986, 33, 201-217; J. Pharm.ScL, 1997 (Jan), 66, 1, 1 ). Other salts well known to those in the art may, however, be useful in the preparation of compounds according to this invention or of their pharmaceutically acceptable salts. Representative organic or inorganic acids include, but are not limited to, hydrochloric, hydrobromic, hydriodic, perchloric, sulfuric, nitric, phosphoric, acetic, propionic, glycolic, lactic, succinic, maleic, fumaric, malic, tartaric, citric, benzoic, mandelic, methanesulfonic, hydroxyethanesulfonic, benzenesulfonic, oxalic, pamoic, 2-naphthalenesulfonic, p-toluenesulfonic, cyclohexanesulfamic, salicylic, saccharinic or trifluoroacetic acid. Representative organic or inorganic bases include, but are not limited to, basic or cationic salts such as benzathine, chloroprocaine, choline, diethanolamine, ethylenediamine, meglumine, procaine, aluminum, calcium, lithium, magnesium, potassium, sodium and zinc.

The present invention includes within its scope prodrugs of the compounds of this invention. In general, such prodrugs will be functional derivatives of the compounds that are readily convertible in vivo into the required compound. Thus, in the methods of treatment of the present invention, the term "administering" shall encompass the treatment of the various disorders described with the compound specifically disclosed or with a compound which may not be specifically disclosed, but which converts to the

specified compound in vivo after administration to the patient. Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs", ed. H. Bundgaard, Elsevier, 1985.

Where the compounds according to this invention have at least one chiral center, they may accordingly exist as enantiomers. Where the compounds possess two or more chiral centers, they may additionally exist as diastereomers. It is to be understood that all such isomers and mixtures thereof are encompassed within the scope of the present invention. Furthermore, some of the crystalline forms for the compounds may exist as polymorphs and as such are intended to be included in the present invention. In addition, some of the compounds may form solvates with water (i.e., hydrates) or common organic solvents, and such solvates are also intended to be encompassed within the scope of this invention.

Where the processes for the preparation of the compounds according to the invention give rise to mixture of stereoisomers, these isomers may be separated by conventional techniques such as preparative chromatography. The compounds may be prepared in racemic form, or individual enantiomers may be prepared either by enantiospecific synthesis or by resolution. The compounds may, for example, be resolved into their component enantiomers by standard techniques, such as the formation of diastereomeric pairs by salt formation with an optically active acid, such as (-)-di-p-toluoyl-d-tartaric acid and/or (+)-di-p-toluoyl-l-tartaric acid followed by fractional crystallization and regeneration of the free base. The compounds may also be resolved by formation of diastereomeric esters or amides, followed by chromatographic separation and removal of the chiral auxiliary. Alternatively, the compounds may be resolved using a chiral HPLC column.

During any of the processes for preparation of the compounds of the present invention, it may be necessary and/or desirable to protect sensitive or

reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, ed. J.F.W. McOmie, Plenum Press, 1973; and T.W. Greene & P. G. M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 1991. The protecting groups may be removed at a convenient subsequent stage using methods known from the art.

Even though the compounds of the present invention (including their pharmaceutically, acceptable salts and pharmaceutically acceptable solvates) can be administered alone, they will generally be administered in admixture with a pharmaceutical carrier, excipient or diluent selected with regard to the intended route of administration and standard pharmaceutical or veterinary practice. Thus, the present invention is directed to pharmaceutical and veterinary compositions comprising compounds of Formula (I) and one or more pharmaceutically acceptable carriers, excipients or diluents.

By way of example, in the pharmaceutical and veterinary compositions of the present invention, the compounds of the present invention may be admixed with any suitable binder(s), lubricant(s), suspending agent(s), coating agent(s), and/or solubilising agent(s).

Tablets or capsules of the compounds may be administered singly or two or more at a time, as appropriate. It is also possible to administer the compounds in sustained release formulations.

Alternatively, the compounds of the general Formula (I) can be administered by inhalation or in the form of a suppository or pessary, or they may be applied topically in the form of a lotion, solution, cream, ointment or dusting powder. An alternative means of transdermal administration is by use of a skin patch. For example, they can be incorporated into a cream consisting of an aqueous emulsion of polyethylene glycols or liquid paraffin. They can also be incorporated, at a concentration of between 1 and 10% by weight, into an

ointment consisting of a white wax or white soft paraffin base together with such stabilizers and preservatives as may be required.

For some applications, preferably the compositions are administered orally in the form of tablets containing excipients such as starch or lactose, or in capsules or ovules either alone or in admixture with excipients, or in the form of elixirs, solutions or suspensions containing flavoring or coloring agents.

The compositions (as well as the compounds alone) can also be injected parenterally, for example intracavemosally, intravenously, intramuscularly or subcutaneously. In this case, the compositions will comprise a suitable carrier or diluent.

For parenteral administration, the compositions are best used in the form of a sterile aqueous solution which may contain other substances, for example enough salts or monosaccharides to make the solution isotonic with blood.

For buccal or sublingual administration the compositions may be administered in the form of tablets or lozenges which can be formulated in a conventional manner.

By way of further example, pharmaceutical and veterinary compositions containing one or more of the compounds of the invention described herein as the active ingredient can be prepared by intimately mixing the compound or compounds with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier may take a wide variety of forms depending upon the desired route of administration (e.g., oral, parenteral). Thus for liquid oral preparations such as suspensions, elixirs and solutions, suitable carriers and additives include water, glycols, oils, alcohols, flavoring agents, preservatives, stabilizers, coloring agents and the like; for solid oral preparations, such as powders, capsules and tablets, suitable carriers and additives include starches, sugars, diluents, granulating agents, lubricants,

binders, disintegrating agents and the like. Solid oral preparations may also be coated with substances such as sugars or be enteric-coated so as to modulate the major site of absorption. For parenteral administration, the carrier will usually consist of sterile water and other ingredients may be added to increase solubility or preservation. Injectable suspensions or solutions may also be prepared utilizing aqueous carriers along with appropriate additives.

Advantageously, compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal skin patches well known to those skilled in that art. To be administered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen.

It is also apparent to one skilled in the art that the therapeutically effective dose for active compounds of the invention or a pharmaceutical composition thereof will vary according to the desired effect. Therefore, optimal dosages to be administered may be readily determined and will vary with the particular compound used, the mode of administration, the strength of the preparation, and the advancement of the disease condition. In addition, factors associated with the particular subject being treated, including subject age, weight, diet and time of administration, will result in the need to adjust the dose to an appropriate therapeutic level. The above dosages are thus exemplary of the average case. There can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention.

Compounds of this invention may be administered in any of the foregoing compositions and dosage regimens or by means of those compositions and

dosage regimens established in the art whenever use of the compounds of the invention as analgesics is required for a subject in need thereof.

The invention also provides a pharmaceutical or veterinary pack or kit comprising one or more containers filled with one or more of the ingredients of the pharmaceutical and veterinary compositions of the invention. Optionally associated with such container(s) can be a notice in the form prescribed by a governmental agency regulating the manufacture, use or sale of pharmaceuticals or biological products, which notice reflects approval by the agency of manufacture, use or sale for human administration.

The compounds of the present invention may be used to treat mild to severe pain in warm-blooded animals such as humans by administration of an analgesically effective dose. The dosage range would be from about 0.1 mg to about 15,000 mg, in particular from about 50 mg to about 3500 mg or, more particularly from about 100 mg to about 1000 mg of active ingredient in a regimen of about 1 to 4 times per day for an average (70 kg) human; although, it is apparent to one skilled in the art that the therapeutically effective amount for active compounds of the invention will vary as will the types of pain being treated.

For oral administration, a pharmaceutical composition is preferably provided in the form of tablets containing 0.01 , 10.0, 50.0, 100, 150, 200, 250, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the subject to be treated.

Examples of pain intended to be within the scope of the present invention include, but are not limited to, inflammatory pain, centrally mediated pain, peripherally mediated pain, visceral pain, structural or soft tissue injury related pain, progressive disease related pain, neuropathic pain and acute pain such as caused by acute injury, trauma or surgery and chronic pain such as headache and that caused by neuropathic conditions, post-stroke conditions,

cancer, and migraine.

Compounds of the present invention are also useful as immunosuppressants, antiinflammatory agents, agents for the treatment and prevention of neurological and psychiatric conditions, for instance, depression and Parkinson's disease, agents for the treatment of urological and reproductive conditions, for instance, urinary incontinence and premature ejaculation, medicaments for drug and alcohol abuse, agents for treating gastritis and diarrhea, cardiovascular agents and cardioprotective agents and agents for the treatment of respiratory diseases.

The compounds of the present invention are also useful in treating pain caused by osteoarthritis, rheumatoid arthritis, fibromyalgia, migraine, headache, toothache, burn, sunburn, snake bite (in particular, venomous snake bite), spider bite, insect sting, neurogenic bladder, benign prostatic hypertrophy, interstitial cystitis, rhinitis, contact dermatitis/hypersensitivity, itch, eczema, pharyngitis, mucositis, enteritis, cellulites, causalgia, sciatic neuritis, mandibular joint neuralgia, peripheral neuritis, polyneuritis, stump pain, phantom limb pain, post-operative ileus, cholecystitis, postmastectomy pain syndrome, oral neuropathic pain, Charcot's pain, reflex sympathetic dystrophy, Guillain-Barre syndrome, meralgia paresthetica, burning-mouth syndrome, post-herpetic neuralgia, trigeminal neuralgia, cluster headache, migraine headache, peripheral neuropathy, bilateral peripheral neuropathy, diabetic neuropathy, postherpetic neuralgia, trigeminal neuralgia, optic neuritis, postfebrile neuritis, migrating neuritis, segmental neuritis, Gombault's neuritis, neuronitis, cervicobrachial neuralgia, cranial neuralgia, geniculate neuralgia, glossopharyngial neuralgia, migrainous neuralgia, idiopathic neuralgia, intercostals neuralgia, mammary neuralgia, Morton's neuralgia, nasociliary neuralgia, occipital neuralgia, red neuralgia, Sluder's neuralgia, splenopalatine neuralgia, supraorbital neuralgia, vidian neuralgia, inflammatory bowel disease, irritable bowel syndrome, sinus headache, tension headache, labor, childbirth,

menstrual cramps, and cancer.

In regard to the use of the present compounds in treatment of the disases or conditions such as those listed above, a therapeutically effective dose can be determined by persons skilled in the art by the use of established animal models. Such a dose would likely fall in the range of from about 0.01 mg to about 15,000 mg of active ingredient administered 1 to 4 times per day for an average (70 kg) human.

GENERAL SYNTHETIC METHODS

Representative compounds of the present invention can be synthesized in accordance with the general synthetic methods described below and are illustrated in the schemes that follow. Since the schemes are an illustration, the invention should not be construed as being limited by the chemical reactions and conditions expressed. The preparation of the various starting materials used in the schemes is well within the skill of persons versed in the art.

Scheme 1

The preparation of compounds of this invention is illustrated in Schemes 1 through 11. The overall strategy in Scheme 1 is based on the synthesis of

appropriately substituted compounds of formula 1.4 (Y =0, S) that are condensed with an appropriately substituted compound of formula 1.5. In compounds of formula 1.5, X ₂ and X ₃ can each be a halogen atom, trifluoromethanesulfonyloxy or a nitro group. In stage 1.1 , a bridged Λ/1- protected 4-aminopiperidine 1.1 is condensed with a properly substituted O- protected phenol (Y=O) or thiophenol (Y=S) 1.2. The protective group on the N1 nitrogen of 1.1 (represented as P) may include an alkanyl, alkenyl or aralkanyl group in which case they are the therapeutically useful products of this invention. The group P may also be trifluoromethylcarbonyl, alkoxycarbonyl or aralkoxycarbonyl. Bridge A may include (CH ₂ ) 2 and (CH ₂ ) ₃ . Useful phenol or thiophenol protective groups (R) include lower alkyl groups, benzyl, trialkylsilyl and the like. Appropriate substituents on the protected phenol or thiophenol in the 2-position (X-O may include halogens and trifluoromethanesulfonyloxy. The Q group in the 5-position may be a substituent such as fluoro, chloro, bromo, cyano, iodo, carboxy, dialkylaminosulfonyl or trifluoromethanesulfonyloxy. Stage 1.2 includes deprotection of the phenol or thiophenol protective group. Such transformations may include the dealkylation of lower alkyl ethers to give their corresponding alcohols using reagents such as boron trihalides, or dealkylation of lower alkyl thioethers using reagents such as Na/NH ₃ . A benzyl protective group may be removed under conditions of hydrogenation in the presence of a transition metal such as palladium. Trialkylsilyl protective groups may be removed by treatment with a source of fluoride anion such as tetrabutyl ammonium fluoride, or by exposure to an inorganic acid such as aqueous hydrogen chloride and the like.

In stage 1.3, hydroxyaniline (Y=O) or thioaniline (Y=S) 1.4 may be condensed with an appropriately substituted benzene moiety 1.5. Substituents X ₂ and X ₃ may include halogens, trifluoromethanesulfonyloxy, or a nitro group. Useful coupling conditions of the anilino nitrogen with a compound of formula 1.5 include palladium catalyzed condensations in the presence of a phosphine ligand such as Pd ₂ (dba) ₃ and a base such as cesium carbonate. Coupling of

the hydroxy or thio moiety with the remaining substituted phenyl group may proceed using Ullmann type coupling conditions. In addition, the two steps described in stage 1.3 may be reversed with biaryl ether or biaryl thioether formation preceding the formation of the biaryl amine. Alternatively, the condensation between compounds of formula 1.4 and compounds of formula 1.5 to yield compounds of formula 1.6 in one step may be affected by treatment with an inorganic base such as potassium carbonate in a suitable solvent such as dimethyl formamide.

The regiochemical outcome of the condensation between compounds of formula 1.4 and compounds of formula 1.5 depends on the position of the R4 substituent in compounds of formula 1.5 and on the reaction conditions used for the condensation. An extensive review on this topic is available in the literature (see, for eample: The Smiles and Related Rearrangements of aromatic Systems' by W. E. Truce, E. M. Kreider, and W. W. Brand in Organic Reactions, 1970, Vol. 18, pp.99-215).

The protective group P can be removed to obtain secondary amines 1 as illustrated for Stage 1.4. These transformations may be carried out using certain acidic reagents such as hydrogen bromide or trimethylsilyl iodide. Phenoxazines (Y=O) or phenothiazines (Y=S) of type 1.6 bearing readily cleavable groups such as methyl, allyl or benzyl may be transformed into the aforementioned alkoxycarbonyl derivatives by treatment with alkanylchloroformates such as ethyl chloroformate or 1 -chloroethyl chloroformate and thus serve as sources of phenoxazines and phenothiazines 1. Phenoxazines or phenothiazines of type 1.6 bearing a trifluormethylcarbonyl group may be treated with potassium carbonate in an alcoholic solvent such as methanol to yield phenoxazines and phenothiazines 1.

Finally the secondary amines 1 may be converted to a compound of formula 2 as shown in Stage 1.5. These transformations may be carried out by reductive alkylation using a carbonyl compound and a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride, or tetramethylammonium triacetoxyborohydride. They may also be carried out

by alkylation using an alkanyl, alkenyl or aralkyl halide and an organic or inorganic base.

The Q function in compounds 1 or 2 may be converted into group G, which may be -C(Z)NRiR ₂ , an aryl substituent, or an appropriate heterocycle as defined herein, to give compounds of formula I. When the Q function is a halogen or trifluoromethanesulfonyloxy, it may be converted to an ester via alkoxycarbonylation using carbon monoxide, an aliphatic alcohol, a trialkanyl amine, and a palladium catalyst such as bis(triphenylphosphine) palladium(ll)dichloride. Subsequently, when Q is an ester, the ester may be hydrolyzed to a carboxylic acid. The carboxylic acid may then be coupled with ammonia, a primary amine, or a secondary amine to form a primary, secondary or tertiary amide, respectively. Alternatively, the conversion of a carboxylic acid to an amide may be carried out via an acid chloride using thionyl chloride, oxalyl chloride, or the like, followed by a Schotten-Baumann reaction using ammonia or an amine in the presence of an alkali metal hydroxide. Alternatively, the conversion of a carboxylic acid to an amide may be carried out via the use of peptide coupling agents such as 1 ,3- dicyclohexylcarbondiimide (DCC), O-(7-azabenzotriazol-1 -yl)-N,N,N',N'- tetramethyluronium hexafluorophosphate (HATU), O-benzotriazol-1 -yl- Λ/,Λ/,Λ/',/V-tetramethyluronium hexafluorophosphate (HBTU), or the like. Alternatively, the ester may be converted directly to the amide by the action of a dimethylaluminum amide.

Alternatively, when the Q function is a halogen or trifluoromethanesulfonyloxy, it may be converted directly to an amide via aminocarbonylation using a carbon monoxide source such as molybdenum hexacarbonyl, an appropriate amine, and a palladium catalyst such as Hermann's catalyst.

Alternatively, one may effect the transformation of the group Q to a substituent G (wherein G is an amidino or heterocycle) by way of a nitrile. Synthesis of the nitrile may be accomplished by treatment of the compounds 1 or 2 (when Q is bromo or trifluoromethanesulfonyloxy) with Zn(CN) ₂ and a

palladium catalyst such as (Ph ₃ P) ₄ Pd or by treatment of the compounds 1 or 2 with CuCN at elevated temperatures. For the synthesis of amidino functional groups, the nitrile is treated with hydroxylamine under basic conditions to afford an oxime. Treatment of the oxime with a primary or secondary amine, CuCI, and an alkali metal carbonate under microwave irradiation in an alcoholic solvent provides the amidino compounds of the present invention. Microwave accelerated reactions may be performed using either a CEM Discover or a Personal Chemistry Smith Synthesizer microwave instrument. The oxime described above is instrumental in the preparation of compounds wherein G is a heterocycle. The oxime may be cyclized with a variety of electrophiles known to one versed in the art to give the heterocycles of the present invention. For instance, reaction of an oxime with CDI provides oxadiazolones, and treatment of the oxime with TCDI provides the corresponding oxadiazolethiones. Similarly, the treatment of the oxime with thionyl chloride in the presence of a tertiary amine gives oxathiadiazoles of the present invention.

Alternatively, compounds where Q is a halogen atom or a trifluoromethanesulfonyloxy group may participate in transition metal-mediated coupling reactions such as Suzuki, Stille or Negishi chemistry. Desired end products of the present invention may include chemical modifications at R ₄ . Such transformations may include the dealkylation of lower alkyl ethers to give the corresponding alcohols using reagents such as boron trihalides. Compounds where R ₄ is a halogen atom may participate in transition metal-mediated coupling reactions such as Suzuki, Stille or Negishi chemistry.

Scheme 2

Scheme 2 outlines an alternative approach to the synthesis of phenoxazines (Y=O) or phenothiazines (Y=S) 1.6. In this scheme, an appropriately substituted phenol (Y=O) or thiophenol (Y=S) of type 2.2 is reacted with an appropriately substituted benzene moiety 2.1 in the presence of a base, such as potassium carbonate or sodium hydroxide in an organic solvent, such as dimethyl formamide, dimethyl acetamide, dimethyl sulfoxide or the like as shown in stage 2.1. Appropriate substituents Xi and X ₂ in this scheme may include halogens and trifluoromethanesulfonyloxy. In stage 2.2, the nitro functionality is reduced to the corresponding amine. This reduction can be accomplished via treatment with tin(ll) chloride in an alcoholic solvent such as ethanol. Stage 2.3 depicts the conversion of primary aniline 2.4 to secondary aniline 2.6, which can be accomplished via reductive alkylation using a carbonyl compound 2.5 and a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride, or tetramethylammonium triacetoxyborohydride. Stage 2.4 depicts formation of compounds of formula 1.6, which can be accomplished by treatment of secondary aniline 2.6 with an appropriate base such as potassium carbonate.

Scheme 3

3.1 3.2 2.3

Scheme 3 illustrates an alternative synthesis of compound 2.3. In this approach, an appropriately substituted 2-nitrophenol (Y=O) or 2-nitrothiophenol (Y=S) may be condensed with an appropriately substituted benzene moiety of type 3.2 under Ullmann type coupling conditions. Appropriate substituents Xi and X2 include halogens and trifluoromethanesulfonyloxy.

Scheme 4

Scheme 4 illustrates an alternative approach to the synthesis of phenoxazines (Y=O) or phenothiazines (Y=S) 1.6. Condensation of appropriately substituted phenols (Y=O) orthiophenols (Y=S) 2.2 with substituted benzene moiety 1.5 under Ullmann type coupling conditions as shown in stage 4.1 may result in the formation of biaryl ethers (Y=O) or biaryl thioethers (Y=S) 4.1. Appropriate Xi, X ₂ and X ₃ substituents may include halogens and trifluoromethanesulfonyloxy. Palladium catalyzed condensation of biaryl ethers or biaryl thioethers 4.1 with bridged Λ/1 -protected 4-

aminopiperidines 1.1 in the presence of a phosphine ligand such as Pd ₂ (dba) ₃ and a base such as cesium carbonate is shown in stage 4.2 may result in the formation of phenoxazines or phenothiazines 1.6.

Scheme 5

An alternative approach to the synthesis of intermediate 4.1 is depicted in Scheme 5 and is based on the reaction of appropriately substituted phenols (Y=O) or thiophenols (Y=S) 5.1 with an appropriately substituted benzene moiety 3.2 under Ullmann type coupling conditions (stage 5.1). Substituents X- _I , X ₂ , and X ₃ may include halogens or trifluoromethanesulfonyloxy.

Scheme 6 illustrates an alternative approach to the synthesis of intermediates 2.6. An appropriately substituted compound of formula 1.5 may be reacted with bridged Λ/1 -protected 4-aminopiperidines 1.1 in the presence of a palladium catalyst such as Pd ₂ (dba) ₃ , a phosphine ligand and a base such as cesium carbonate as shown in stage 6.1. Appropriate Xi and X ₂ substituents may include halogens and trifluoromethanesulfonyloxy. Compounds of formula 6.1 may then be reacted with appropriately substituted phenols (Y=O) or

thiophenols (Y=S) 2.2 under Ullmann type coupling conditions to yield anilines 2.6 as shown in stage 6.2.

Scheme 7

Scheme 7 illustrates an alternative approach to the synthesis of phenoxazines (Y=O) or phenothiazines (Y=S) 1.6. Condensation of appropriately substituted compounds of formula 3.2 with bridged Λ/1 -protected 4-aminopiperidines 1.1 may result in formation of intermediate 7.1 as shown in stage 7.1. Appropriate X ₁ , X ₂ , and X ₃ substituents may include halogens and trifluoromethanesulfonyloxy. Reaction of compounds 7.1 with appropriately substituted phenol (Y=O) or thiophenols (Y=S) 7.2 under Ullmann like coupling conditions may result in formation of compounds 7.3 as shown in stage 7.2. Finally, ring closure of compounds 7.4 may be accomplished in the presence of a palladium catalyst such as Pd ₂ (dba) ₃ , a phosphine ligand such as 4,5- bis(diphenylphosphino)-9,9-dimethylxanthene (xant phos) and a base such as potassium tert-butoxide or cesium carbonate as shown in stage 7.3.

Scheme 8 illustrates an alternative synthesis of phenoxazines (Y=O) or phenothiazines (Y=S) 1.6 and is based on an Ullmann-like coupling of bridged Λ/1 -protected 4-aminopiperidines 1.1 with appropriately substituted and protected phenols (Y=O) or thiophenols (Y=S) 8.1 as shown in stage 8.1. Useful phenol or thiophenol protective groups for compounds 8.1 include lower alkyl groups, benzyl, trialkylsilyl and the like. Appropriate X-i, X ₂ , and X ₃ substituents may include halogens and trifluoromethanesulfonyloxy. The resulting compounds 8.2 may be condensed with appropriately functionalized benzene compounds 3.2 to yield diarylanilines 8.3. Stage 8.3 includes deprotection of the phenol or thiophenol protective group. Such transformations may include the dealkylation of lower alkyl ethers to give their corresponding alcohols using reagents such as boron trihalides or the dealkylation of the alkyl thioethers using Na/NH ₃ . A benzyl protective group may be removed under conditions of hydrogenation in the presence of a transition metal such as palladium. Trialkylsilyl protective groups may be removed by treatment with a source of fluoride anion such as tetrabutyl ammonium fluoride, or by exposure to an inorganic acid such as aqueous hydrogen chloride and the like. Finally, ring closure of compounds 8.3 to phenoxazines or phenothiazines 1.6 may be accomplished via an Ullmann type

transformation as shown in stage 8.4.

Scheme 9 illustrates another synthetic approach to compounds 7.4. Displacement of Xi in appropriately substituted compounds of formula 9.2 with appropriately substituted compounds of formula 9.1 as shown in stage 9.1 may lead to biaryl ethers (Y=O) and biarylthioethers (Y=S) 9.3. Appropriate Xi and X ₂ substituents may include halogens and trifluoromethanesulfonyloxy. Reduction of compounds 9.3 to amines 9.4 as shown in stage 9.2 may be accomplished using tin(ll) chloride in an alcoholic solvent such as ethanol. Stage 9.3 depicts the conversion of primary anilines 9.4 to secondary anilines 7.4 and can be accomplished via reductive alkylation using a carbonyl compounds 2.5 and a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride, or tetramethylammonium triacetoxyborohydride.

Scheme 10

Scheme 10 illustrates another synthetic approach to compounds 9.3. For construction of diaryl ethers (Y=O) and diaryl thioethers (Y=S) 9.3, appropriately substituted 2-hydroxynitrobenzenes or 2-thionitrobenzenes 10.1 may be caused to react with appropriately substituted compounds of formula 1.5 under Ullmann type conditions as shown in stage 10.1. Appropriate X ₂ and X ₃ substituents may include halogens and trifluoromethanesulfonyloxy.

Scheme 11

Scheme 11 illustrates another synthetic approach to compounds 1.6. Stage 11.1 depicts the conversion of appropriately substituted 2-hydroxyanilines (Y=O) 11.1 to compounds 11.2 (Y=O), which can be accomplished via reductive alkylation using a carbonyl compound 2.5 and a reducing agent such as sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride, or tetramethylammonium triacetoxyborohydride. Appropriately substituted 2-hydroxyanilines 11.2 may be caused to react with an appropriately substituted benzene 3.2 under Ullmann type conditions or under basic conditions such as potassium carbonate in DMF (when X ₁ = NO ₂ ) as shown in stage 11.3 to yield compounds of formula 1.6. Appropriate Xi and

X ₂ substituents may include halogens, trifluoromethanesulfonyloxy, and a nitro group.

The regiochemical outcome of the condensation of compounds of formula 11.2 with compounds of formula 3.2 depends on the position of the Q substituent in compounds of formula 3.2 and on the reaction conditions used for the condensation. An extensive review on this topic is available in the literature (see, for eample: The Smiles and Related Rearrangements of aromatic Systems' by W. E. Truce, E. M. Kreider, and W. W. Brand in Organic Reactions, 1970, Vol. 18, pp.99-215).

In compounds of formula 11.1 where Y is sulfur, an intermediate spiro compound of formula 11.3 may be formed. Compounds of formula 11.3 may be converted to compounds of formula 11.2 (Y = S) by treatment with a hydride reagent such as lithium aluminum hydride or sodium borohydride.

In the above Schemes 1 through 11 , the Q function of compounds 2 may be converted into group G, which may be -C(Z)NRiR ₂ , an aryl substituent, or an appropriate heterocycle as defined herein, to give compounds of formula 3. When the Q function of compounds 2 is a halogen or trifluoromethanesulfonyloxy, it may be converted to an ester via alkoxycarbonylation using carbon monoxide, an aliphatic alcohol, a trialkanyl amine, and a palladium catalyst such as bis(triphenylphosphine) palladium(ll)dichloride. Subsequently, when Q is an ester, the ester may be hydrolyzed to a carboxylic acid. The carboxylic acid may then be coupled with ammonia, a primary amine, or a secondary amine to form a primary, secondary or tertiary amide, respectively. Alternatively, the conversion of a carboxylic acid to an amide may be carried out via an acid chloride using thionyl chloride, oxalyl chloride, or the like, followed by a Schotten-Baumann reaction using ammonia or an amine in the presence of an alkali metal hydroxide. Alternatively, the conversion of a carboxylic acid to an amide may be carried out via the use of peptide coupling agents such as 1 ,3- dicyclohexylcarbondiimide (DCC), O-(7-azabenzotriazol-1-yl)-Λ/,Λ/,Λ/',Λ/-

tetramethyluronium hexafluorophosphate (HATU), O-benzotriazol-1 -yl- Λ/,N,Λ/',/V-tetramethyluronium hexafluorophosphate (HBTU), or the like. Alternatively, the ester may be converted directly to the amide by the action of a dimethylaluminum amide.

Instead of proceeding to compounds 3 via an ester, one may effect the transformation of the group Q to a substituent G (wherein G is an amidino or heterocycle) by way of a nitrile. Synthesis of the nitrile may be accomplished by treatment of the compounds 2 (when Q is bromo or trifluoromethanesulfonyloxy) with Zn(CN) ₂ and a palladium catalyst such as (Ph ₃ P) ₄ Pd or by treatment of the compounds 2 with CuCN at elevated temperatures. For the synthesis of amidino functional groups, the nitrile is treated with hydroxylamine under basic conditions to afford an oxime. Treatment of the oxime with a primary or secondary amine, CuCI, and an alkali metal carbonate under microwave irradiation in an alcoholic solvent provides the amidino compounds of the present invention. Microwave accelerated reactions may be performed using either a CEM Discover or a Personal Chemistry Smith Synthesizer microwave instrument. The oxime described above is instrumental in the preparation of compounds wherein G is a heterocycle. The oxime may be cyclized with a variety of electrophiles known to one versed in the art to give the heterocycles of the present invention. For instance, reaction of an oxime with CDI provides oxadiazolones, and treatment of the oxime with TCDI provides the corresponding oxadiazolethiones. Similarly, the treatment of the oxime with thionyl chloride in the presence of a tertiary amine gives oxathiadiazoles of the present invention.

An aryl substituent may be installed in place of the functional group Q by coupling compounds 2 (when Q is bromo or trifluoromethanesulfonyloxy) with a suitably substituted arylboronic acid in the presence of a palladium catalyst and an alkali metal carbonate.

Desired end products of the present invention may include chemical modifications at R ₄ . Such transformations may include the dealkylation of lower alkyl ethers to give their corresponding alcohols, using reagents such as

boron trihalides. Compounds where R ₄ is a halogen atom may participate in transition metal-mediated coupling reactions such as Suzuki, Stille or Negishi chemistry.

It is generally preferred that the respective product of each process step be separated from other components of the reaction mixture and subjected to purification before its use as a starting material in a subsequent step. Separation techniques typically include evaporation, extraction, precipitation and filtration. Purification techniques typically include column chromatography (Still, W. C. et. al., J. Org. Chem. 1978, 43, 2921), thin-layer chromatography, crystallization and distillation. The structures of the final products, intermediates and starting materials are confirmed by spectroscopic, spectrometric and analytical methods including nuclear magnetic resonance (NMR), mass spectrometry (MS) and liquid chromatography (HPLC). In the descriptions for the preparation of compounds of this invention, ethyl ether, tetrahydrofuran and dioxane are common examples of an ethereal solvent; benzene, toluene, hexanes and heptanes are typical hydrocarbon solvents and dichloromethane and dichloroethane are representative halogenated hydrocarbon solvents. In those cases where the product is isolated as the acid addition salt the free base may be obtained by techniques known to those skilled in the art. In those cases in which the product is isolated as an acid addition salt, the salt may contain one or more equivalents of the acid. Enantiomers of the compounds of the present invention may be separated using chiral HPLC.

Representative compounds of the present invention can be synthesized in accordance with the general synthetic methods described above and are illustrated more particularly in the schemes that follow. Since the schemes are illustrations, the invention should not be construed as being limited by the chemical reactions and conditions expressed. The preparation of the various starting materials used in the schemes is well within the skill of persons versed in the art.

Abbreviations

AcOH acetic acid

Boc = fe/t-butoxycarbonyl

DIEA Λ/,Λ/-diisopropyl-Λ/-ethylamine

DMF Λ/,Λ/-dimethylformamide

DMSO dimethyl sulfoxide

Et ethyl h hour(s)

HBTU 0-benzotriazol-1-yl-Λ/,Λ/,N',Λ/4etramethyluronium hexafluorophosphate

K ₂ CO ₃ potassium carbonate

Me methyl min = minute(s) rt room temperature xantphos = 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene

Although the foregoing invention has been described in detail by way of example for purposes of clarity of understanding, it will be apparent to the artisan that certain changes and modifications are comprehended by the disclosure and can be practiced without undue experimentation within the scope of the appended claims, which are presented by way of illustration not limitation.

All publications and patent documents cited above are hereby incorporated by reference in their entirety for all purposes to the same extent as if each were so individually denoted.

EXAMPLES

Example A

Procedure 1

3-Hydroxy-4-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)- benzoic acid methyl ester, 1a

To a solution of 4-amino-3-hydroxybenzoic acid methyl ester (2.Og; 12 mmol) and 8-methyl-8-aza-bicyclo[3.2.1]octan-3-one (1.1g; 10.8 mmol) in dichloroethane (30 ml_) was added sodium triacetoxyborohydride (3.2g; 15.1 mmol) and acetic acid (0.65 ml_; 11.4 mmol). The mixture was stirred at rt for 16 h. The mixture was diluted with 1 N NaHCO ₃ solution and chloroform was added. The organic layer separated, and the aqueous phase was lyophilized. The lyophilized residue was purified via reverse phase HPLC (eluent gradient: 10 to 50% acetonitrile in water containing 0.1% TFA) to yield 1.61g (36.9%) of a mixture of endo and exo isomers of 3-hydroxy-4-(8-methyl-8-aza-

bicyclo[3.2.1]oct-3-ylamino)-benzoic acid methyl ester, 1a as a TFA salt. MS m/z (MH ⁺ ) 291.

Procedure 2

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-f0H-phenoxazin e-3-carboxylic acid methyl ester, 2a

A mixture of the TFA salt of 3-hydroxy-^8-methyl-8-aza-bicyclo[3.2.1]oct-3- ylamino)-benzoic acid methyl ester, 1a (0.424g, 0.734 mmol), 2- fluoronitrobenzene (95 μl_, 0.9 mmol) and potassium carbonate (564 mg, 4 mmol) in DMF was heated to reflux for 70 min. The mixture was allowed to cool to rt, filtered, and purified via reverse phase HPLC to yield 440 mg (88%) of 10-(8~methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3 -carboxylic acid methyl ester, 2a, as TFA salt. MS m/z (MH ⁺ ) 365.1.

Procedure 3

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin e-3-carboxylic acid, 3a

To a solution of 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3 - carboxylic acid methyl ester, 2a (440 mg, 0.646 mmol) in THF (5 mL) was added 1 N NaOH (5 mL), and the mixture was stirred for 3 hr at rt. The solvent was removed, the residue was dissolved in DMF and acidified with a 2N TFA solution, and purified via reverse phase HPLC to yield 295 mg (63%) of 10-(8- methyl-8-aza~bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbo xylic acid, 3a, as a TFA salt. MS m/z (MH ⁺ ) 351.1.

Procedure 4

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin e-3-carboxyIic acid diethylamide, 4a

To a solution of the TFA salt of 10-^-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3-carboxylic acid 3a (65 mg, 0.14 mmol) and HBTU (64 mg; 0.17 mmol) in DMF (2 mL) was added triethylamine (69 μL, 0.43 mmol). The

mixture was stirred for 1 hr at rt, and purified without prior quenching via reverse phase HPLC (eluent gradient: acetonitrile in water containing 0.1 % TFA) to yield 44.4 mg (61 %) of title compound 10-(8-methyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 4a as a TFA salt. MS m/z (MH ⁺ ) 406.2.

(3-Hydroxy-pyrrolidin-i-yl)-fiO-tβ-methyl-8-aza-bicyclo[ 3.2.1]oct-3-yl)- 10H- phenoxazin-3-yl)-methanone, 5a

Using an adaptation of Procedure 4, and substituting 3- hydroxypyrrolidine for diethylamine, the title compound (3-hydroxy-pyrrolidin-1- yl)-tiO^8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazi n-3-yl)- methanone, 5a was obtained as a TFA salt. MS m/z (MH ⁺ ) 420.1.

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10W-phenoxazin e-3-carboxyllc acid methyl-phenethyl-amide,

Using an adaptation of Procedure 4, and substituting Λ/-methyl-Λ/- phenethylamine for diethylamine, the title compound 10-(8-methyI-8-aza- bicyclo[3.2.1]oct-3-yl)-1 OH-phenoxazine-3-carboxyIic acid methyl-phenethyl- amide, 6a was obtained as a TFA salt. MS m/z (MH ⁺ ) 468.3.

Example B

Procedure 5

Endo-3-(2-Hydroxy~phenylamino)-8-aza-bicyclo[3.2.1]octane -8-carboxylic acid tert-butyl ester, 1 b and Exo-3-(2-Hydroxy-phenyIamino)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2b To a solution of 2-aminophenol (5.Og; 45.82 mmol) and 3-oxo-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester (15.5 g; 68.8 mmol) in dichloroethane (200 ml_) was added acetic acid (2.62 ml_; 45.77 mmol). The mixture was stirred at rt for 1 h and sodium triacetoxyborohydride (11.6g; 54.73 mmol) was added in small portions. The mixture was stirred at rt for 16 h and treated with H ₂ O (200 mL). The organic layer was separated, dried over MgSO4, filtered, and evaporated. The residue was purified via column

chromatography (eluent gradient: 10 to 30 % ethyl acetate in heptane) to yield 8g (55%) of endo-3-(2-hydroxy-phenylamino)-8-aza-bicyclo[3.2.1]octane-8- carboxylic acid tert-butyl ester, 1b and 1.5 g (10%) of exo-3-(2-hydroxy- phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1b. MS /77/Z (MH ⁺ ) 318.9.

Procedure 6

Endo-3-(3-Cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]oct ane-8- carboxylic acid tert-butyl ester, 3b

To a solution of 4-fluoro-3-nitrobenzonitrile (0.29 g; 1.74 mmol) and endo-3-(2- hydroxy-phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1b (0.55 g; 1.73 mmol) in DMF (6 mL) was added potassium carbonate (0.48 g; 3.47 mmol). The mixture was stirred at 170 ⁰ C for 30 min. The mixture was allowed to cool to rt and poured into ice-water 910 mL). The solid was separated via filtration, washed with water, and dried to yield 0.63 g (86%) of endo-3-(3-cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane -8-carboxylic acid tert-butyl ester, 3b. MS m/z (MH ⁺ ) 417.9.The material was used as such for the next reaction.

Procedure 7

Endo-3-t3-tiH-Tetrazol-δ-yl)-phenoxazin- 10-yll-8-aza-bicyclo[3.2.1]octane- 8-carboxylic acid tert-butyl ester, 4b

To a solution of enαfo-3-(3-cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octa ne- 8-carboxylic acid tert-butyl ester, 3b (1 g; 2.4 mmol) in DMF (20 mL) were added sodium azide (0.47 g, 7.23 mmol) and ammonium chloride (0.39 g; 7.29 mmol), and the mixture was heated at 120 °C for 16h. The mixture was allowed to cool to rt, and filtered. The filtrate was acidified with 1 N hydrochloric acid (10 mL) and extracted with ethyl acetate (3 x 10 mL). The combined organic layers were dried over MgSO ₄ , filtered, and evaporated. The residue was used as such for the next reaction.

Procedure 8

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-y l)-10H- phenoxazine, 5b

To a solution of er?c/o-3-[3-(1H-tetrazol-5-yl)-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]-octane-8-carboxylic acid tert-butyl ester, 4b (2.4 mmol) in dioxane (5 mL) was added a 4N hydrochloric acid solution (5 ml_). The mixture was stirred at rt for 16 h. The mixture was filtered, and the filtrate was evaporated. The residue was purified via reverse phase chromatography (eluent gradient: acetonitrile in water containing 0.1 % TFA) to yield crudeendo-

10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3^IH-tetrazol-8-yl)-10H -phenoxazine, 5b as a TFA salt. MS m/z (MH ⁺ ) 360.9. The material was used as such in the next reaction.

Procedure 9 Eπdo-10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl) -3-(1H-tetrazol-5-yl)-

10H-phenoxazine, 6b

To a suspension of the HCI salt of endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)~3-(1H- tetrazol-5-yl)-10H-phenoxazine, 5b (0.2 g; 0.5 mmol) and 2- pyridylcarboxaldehyde (0.071 mL; 0.74 mmol) in dichloroethane (4 mL) was added sodium triacetoxyborohydride (0.13 g; 0.61 mmol). The mixture was stirred at rt for 15 h and a saturated NaHCO ₃ solution (3 mL). The organic layer was separated, and the aqueous phase was extracted with ethyl acetate (5 mL). The combined organic layers were dried over MgSO ₄ , filtered, and evaporated. The residue was purified via reverse phase HPLC (eluent: acetonitrile in water containing 0.1% TFA) to yield 139.8 mg (quant.) of title compound encfo-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl) -3-(1 H- tetrazol-5-yl)-10H-phenoxazine, 6b as a TFA salt. MS m/z (MH ⁺ ) 452.0.

£ndo-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H -tetrazol-5-yl)-

10H-phenoxazine, 7b

Using an adaptation of Procedure 9, and substituting phenylacetaldehyde for 2- pyridyl carboxaldehyde, the title compound endo-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-3^IH-tetrazol-8-yl^iOH-phenoxazine, 7b was obtained as a TFA salt. MS m/z (MH ⁺ ) 464.9.

Endo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3- carbonitrile, 8b

Using an adaptation of Procedure 9, and substituting encfo-3-(3-cyano- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester,

3b for e/7cfo-3-[3-(1 H-tetrazol-5-yl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]- octane-8-carboxylic acid tert-butyl ester, 4b, the title compound eπdo-10-(8- aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbonitrile, 8b was obtained as a TFA salt. MS m/z (MH ⁺ ) 317.9.

Procedure 10

£ndo-3-[3-(Λ/ _Λ Λ/-Diethyl-carbamimidoyl)-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]-octane-8-carboxylic acid tert-butyl ester, 1c

To a solution of methylmagnesium bromide in diethyl ether (3.0 M , 2.4 ml_, 7.2 mmol) under nitrogen was added dropwise a solution of diethylamine (0.749 ml_, 7.19 mmol) in diethyl ether (2 mL). The mixture was heated to reflux for 30 min, cooled to rt, and a suspension of endo-3-(3-cyano-phenoxazin-10-yl)-8- aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3b (1.0 g, 2.4 mmol) was added. The mixture was stirred at 40 ⁰ C for 2 hr and water (10 mL) was added,. The organic layer was separated, and the aqueous layer was further extracted with chloroform (2 x 10 mL). The combined organic layers were dried over magnesium sulfate, filtered, and evaporated, yielding title compound eπdo-3-[3-(/V,Λ/-diethyl-carbamimidoyl)-phenoxazin-10-yl)- 8-aza-bicyclo[3.2.1]- octane-8-carboxylic acid tert-butyl ester 1c. MS m/z (MH ⁺ ) 491.0.The residue was used as such for the next reaction.

Endo-IO-tS-Aza-bicyclo[3.2.1]oct-3-yl)-WjN-diethyl-IOH-pheno xazine-S- carboxamidine, 2c

Using an adaptation of Procedure 8, and substituting enc/o-3-[3-(Λ/,Λ/-Diethyl- carbamimidoyl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]-octane -8-carboxylic acid tert-butyl ester, 4b for enc/o-3-[3-(1H-tetrazol-5-yl)-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]-octane-8-carboxylic acid tert-butyl ester, 4b, the title compound endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-Λ/,Λ/-diethyl-10H-p henoxazine-3- carboxamidine, 2c was obtained as a TFA salt. MS m/z (MH ⁺ ) 390.9.

Endo-NjW-Diethyl-IO-tδ-pyridin^-ylmethyl-δ-aza-bicyclo[ 3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxamidine, 3c

Using an adaptation of Procedure 8, and substituting enofo-10-(8-aza- bicyclo[3.2.1]oct-3-yl)-Λ/,Λ/-diethyl-10H-phenoxazine-3-ca rboxamidine, 2c for enoto-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H-phenoxazine, 5b, the title compound endo-Λ/,Λ/-diethyl-10-(8-pyridin-2-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxamidine, 3c was obtained as a TFA salt. MS m/z (MH ⁺ ) 482.0.

Endo-MW-Diethyl- 10-t8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H- phenoxazine-3-carboxamidine, 4c

Using an adaptation of Procedure 9, substituting eπdo-Λ/,/V-diethyl-10-(8- pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxa zine-3- carboxamidine, 3c for er?do-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-

yl)-10H-phenoxazine, 5b and phenyl acetaldehyde for 2-pyridyl carboxaldehyde, the title compound endo-Λ/,Λ/~diethyl-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxamidine, 4c was obtained as a TFA salt. MS m/z (MH ⁺ ) 495.0.

Example D

Procedure 11

Enc/o-3-[3-(W-Hydroxycarbamimidoyl)-phenoxazin-10-yl)-8-a za- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1d and endo-3-(3-

Carbamoyl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8- carboxylic acid tert-butyl ester, 2d

To a solution of endo-3-(3-cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane - 8-carboxylic acid tert-butyl ester, 3b (1 g; 2.40 mmol) in ethanol (20 mL) were added ammonium hydroxide hydrochloride (0.5 g; 7.19 mmol) and potassium carbonate (0.66 g; 4.78 mmol), and the mixture was heated to reflux for 16 h. The mixture was allowed to cool to rt, water (20 mL) was added, and the mixture was extracted with ethyl acetate (2 x 20 mL). The combined organic layers were dried over MgSO ₄ , filtered, and evaporated, yielding a ~ 2:1 mixture of title compounds eπc/o-3-[3-(Λ/-hydroxycarbamimidoyl)-phenoxazin- 10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1d and e/ic/o-3-(3-carbamoyl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1] octane-8- carboxylic acid tert-butyl ester, 2d. The crude mixture was used as such in the next reaction. 1d: MS m/z (MH ⁺ ) 451.2., 2d: MS m/z (MH ⁺ ) 458.3.

Procedure 12

Endo- 3-r3-(5-Oxo-4.5-dihvdro-π ,2,41oxadiazol-3-vn-phenoxazin-10-yl)-

8-aza-bicvclo[3.2.noctane-8-carboxylic acid tert-butyl ester, 3d To a solution of the 2:1 mixture of endo-3~[3-(Λ/-hydroxycarbamimidoyl)- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1d and endo-3-(3-carbamoyl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]oc tane-8- carboxylic acid tert-butyl ester, 2d (2.33 mmol) in dioxane (20 mL) was added 1 ,1'-carbonyldiimidazole (0.57 g; 3.52 mmol), and the mixture was stirred at 110 ⁰ C for 4 h. The mixture was allowed to cool to rt, and the solution containing a crude mixture of endo- 3-[3-(5-oxo-4,5-dihydro-[1 ,2,4]oxadiazol-3- yl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]-octane-8-carboxyl ic acid tert-butyl ester, 3d (MS m/z (MH ⁺ ) 477.1 )and e/ido-3-(3-carbamoyl-phenoxazin-10-yl)-8- aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2d was used as such in the next reaction.

Endo- 3-I 10-(8-Aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazin-3-yπ^H-

[1,2;4]oxadiazol-5-one, 4d and encfo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3-carboxylic acid amide, 5d

Using an adaptation of Procedure 8, and substituting a mixture of endo- 3-[3-(5- oxo-4,5-dihydro-[1 ,2,4]oxadiazol-3-yl)-phenoxazin~10-yl)-8-aza-bicydo[3.2.1]- octane-8-carboxylic acid tert-butyl ester, 3d and eA7do-3-(3-carbamoyl- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2d for enαfo-3-[3-(1 H-tetrazol-5-yl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]- octane-8-carboxylic acid tert-butyl ester, 4b, the title compounds endo- 3-[10- (8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3-yπ^H-ti ^^joxadiazol-8-one, 4d and enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-ca rboxylic acid amide, 5d were obtained as TFA salts. 4d: MS m/z (MH ⁺ ) 377.0; 5d: MS m/z (MH ⁺ ) 335.9.

Endo-3-I 10-(8-Pyridin-Z-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H- phenoxazin-3-yl)-4H-[1 ,2,4]oxadiazol-5-one, 6d

Using an adaptation of Procedure 9, and substituting endo- 3-[10-(8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3-yl)-4H-[1 ,2,4]oxadiazol-5-one, 4d for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)- 10H-phenoxazine, 5b, the title compound eπc/o-3-[10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazin-3-yl)-4H-[1 ,2,4]oxadiazol-5-one, 6d was obtained as a TFA salt. MS m/z (MH ⁺ ) 467.9.

Endo-3-I10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazin-3- yl]-4H-[1 ,2,4]oxadiazol-5-one, 7d

Using an adaptation of Procedure 9, and substituting endo- 3-[10-(8~aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3-yl)-4H-[1 ,2,4]oxadiazol-5-one, 4d for endo-λ 0-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-1 OH-phenoxazine, 5b, and phenyl acetaldehyde for 2-pyridyl carboxaldehyde, the title compound endo-3-[10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-ph enoxazin-3-yl)-4H- [1 ,2,4]oxadiazol-5-one, 7d was obtained as a TFA salt MS m/z (MH ⁺ ) 480.9.

EXAMPLE E

Procedure 13

1-Fluoro-3-methoxy-2-nitrobenzene, 1e

To a solution of 2,6-difluoronitrobenzene (5 g, 32 mmol) in methanol (50 ml_) was added potassium hydroxide (1.8 g, 32.5 mmol), and the mixture was

heated to reflux for 3 hr. Water was added, and the mixture was extracted with dichloromethane. The organic layer was separated, dried, filtered, and evaporated to yield 5.1 g (95%) of 1-fluoro-3-methoxy-2-nitrobenzene, 1e. MS m/z (MH ⁺ ) 172.

^β -Methoxy- 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3- carboxylic acid methyl ester, 2e

Using an adaptation of Procedure 2, and substituting 1-fluoro-3-methoxy-2- nitrobenzene, 1e for 4-fluoro-3-nitrobenzonitrile, the title compound 6-methoxy- 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3 -carboxylic acid methyl ester, 2e was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 395.

Procedure 14 e-Methoxy- 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10W-phenoxazine-3- carboxylic acid, 3e

To a solution of the TFA salt of e-methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazine-3-carboxylic acid methyl ester, 2e (270 mg, 0.7 mmol) in dioxane (15 mL) was added sodium hydroxide (31 mg, 0.77 mmol) for 10-(8- methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbo xylic acid methyl ester, 2a, the title compound 8-methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenoxazine-3-carboxylic acid, 3e was obtained as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 381.

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin e-3-carboxylic acid diethylamide, 4e Using an adaptation of Procedure 4, and substituting 6- methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno xazine-3- carboxylic acid, 3e for 10-^-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3-carboxylic acid, 3a, Λ/,Λ/-diisopropyl-/V-ethylamine for triethylamine and HATU for HBTU, the title compound 10-(8-methyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 4e was

obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z

(MH ⁺ ) 436.

Procedure 15

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-10W-phenoxazi ne-3-carboxylic acid diethylamide, 5e

To a solution of the TFA salt of 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazine-3-carboxylic acid diethylamide, 4e (110 mg, 0.25 mmol) in methylene chloride (10 mL) was added 1-chloroethyl chloroformate (0.58 ml_, 0.75 mmol). The mixture was heated to reflux for 2h. The mixture was evaporated, dissolved in methanol (5 mL), and heated for 2h at reflux. Water was added, and the solution was extracted with methylene chloride. The organic phase was separated, evaporated, and purified via reverse phase HPLC to yield 69 mg (44.8%) of title compound 10-(8-aza-bicyclo[3.2.1]oct-3- yl)-6-methoxy-10H-phenoxazine-3-carboxylic acid diethylamide, 5e, as a TFA salt. MS m/z= 422 (M+1 ).

6-Methoxy-10-(8φhenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 0/-/-phenoxazine-

3-carboxylic acid diethylamide, 6e

Using an adaptation of Procedure 9, and substituting 10-(8-aza- bicyclo[3.2.1]oct-3-yl)-6-methoxy-1 OH-phenoxazine-3-carboxylic acid diethylamide, 5e for θπdo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl )- 10H-phenoxazine, 5b, and phenyl acetaldehyde for 2-pyridylcarboxaldehyde, the title compound e-methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxylic acid diethylamide, 6e was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z= 526 (M+1 ).

Procedure 16

6-Hydroxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OW- phenoxazine-3-carboxylic acid diethylamide, 7e

To a solution of the TFA salt of 6-methoxy-10-(8-phenethyl-8~aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 6e (55 mg, 0.086 mmol) in 1 ,2-dichloromethane (5 ml_) at 0 ⁰ C was added a 1 M solution of BBr ₃ (0.43 ml_, 0.43 mmol). The mixture was allowed to stir for 2 h at rt. A saturated NaHCO ₃ solution was added, and the organic phase was separated. The aqueous phase was extracted with methylene chloride, and the combined organic phases were dried, filtered, and evaporated. The residue was purified via reverse phase HPLC (eluent: CH3CN in water containing 0.1% TFA) to yield 23 mg (42.8%) of title compound 6-hydroxy-10-(8-phenethyl-8- aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 7e as a TFA salt and as a mixture of endo and exo isomers. MS m/z= 512 (M+1 ).

e-Methoxy- 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10W-phenoxazine-3- carboxylic acid diethylamide, δe

Using an adaptation of Procedure 16, and substituting the TFA salt of 10-(8- methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbo xylic acid diethylamide, 4e for TFA salt of 6-methoxy-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 6e, the title compound 6-methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenoxazine-3-carboxylic acid diethylamide, δe was obtained as a TFA salt and as a mixture of endo and exo isomers after reverse phase chromatography (eluent gradient: acetonitrile in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 422.

2-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)-phenol, 1f

Using an adaptation of Procedure 5, and substituting 8-methyl-8-aza- bicyclo[3.2.1]-octan-3-one for 3-oxo-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, the title compound 2-(8-methyl-8-aza-bicyclo[3.2.1]oct-3- ylamino)-phenol, 1f was obtained as a TFA salt and as a mixture of endo and exo isomers after reverse phase chromatography (eluent gradient: acetonitrile in water containing 0.1% TFA). MS m/z (MH ⁺ ) 232.9.

10-(8-Mθthyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10W-phenoxazi ne-3-carbonitrile,

2f

Using an adaptation of Procedure 6, and substituting the TFA salt of 2-(8- methyl-8-aza-bicyclo[3.2.1]oct-3-ylaminoJ-phenol, 1f for 3-(2-hydroxy- phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1b, the title compound 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine- 3-carbonitrile, 2f was obtained as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 332.0.

10-(8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1//-tetrazol-5 -yl)-10H- phenoxazine, 3f

Using an adaptation of Procedure 7, and substituting 10-(8-methyl-8-aza- bicyclo[3.2.1]oct-3-yl)-1 OH-phenoxazine-3-carbonitrile, 2f for 3-(3-cyano- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2b, the title compound 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol- 5-yl)-10H-phenoxazine, 3f was obtained as a TFA salt and as a mixture of endo and exo isomers after reverse phase chromatography (eluent gradient: acetonitrile in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 375.1.

EXAMPLE G

/Vj/V-Diethyl- 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3- carboxamidine, 1g

Using an adaptation of Procedure 10, and substituting 10-(8-methyl-8- aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbonitrile, 2f for endo-3-(3- cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxy lic acid tert-butyl ester, 3b, the title compound Λ/,Λ/-diethyl-10-(8-methyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazine-3-carboxamidine, 1g was obtained as a TFA salt and as a mixture of endo and exo isomers after reverse phase chromatography (eluent gradient: acetonitrile in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 405.3.

EXAMPLE H

Λ/-Hydroxy-10-(8-methyl-8«aza-bicyclo[3.2.1]oct-3-yl)-1 0H- phenoxazine-3-carboxamidine, 1h

Using an adaptation of Procedure 11 , and substituting 10-(8-methyl-8- aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbonitrile, 2f for endo-3-(3- cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxy lic acid tert-butyl ester, 3b, the title compound Λ/-Hydroxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenoxazine-3-carboxamidine, 1h was obtained. MS m/z (MH ⁺ ) 365.0.

Procedure 17 3-I 10-t8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazin-S-yl)- 4H-[1 ,2,4]oxacliazol-5-one, 2h

To a solution of Λ/-Hydroxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxamidine, 1h (0.66 g; 1.81 mmol) in dioxane (20 ml_) was added 1 ,1'-carbonyldiimidazole (0.44 g; 2.71 mmol) ₍ and the mixture was stirred at 110 ⁰ C for 4 h. The mixture was allowed to cool to rt, and the solvent was evaporated. The residue was purified via reverse phase HPLC (eluent gradient: 20 to 45% CH ₃ CN in H ₂ O containing 0.1% TFA) to yield 161 mg (17.6%) of title compound 3-[10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenoxazin-3-yl]~4H-[1 ,2,4]oxadiazol-5-one, 2h as a TFA salt. MS m/z (MH ⁺ ) 390.9.

EXAMPLE

Procedure 18

Spiro compound, 1i

A mixture of 2-aminothiophenol (4.3 ml_, 39.9 mmol) and tropinone (5.6 g, 39.9 mmol) was allowed to stir and sit for 16 h at it The mixture was placed

under vacuum (<0.3 mm Hg) for 6 h. HPLC-MS analysis revealed -60% conversion to desired compound, 1i. The material was used as such for the next reaction.

Procedure 19

[2-(4-Bromo-2-nitrophenylsulfanyl)-phenyl]-(8-methyl-8-az a- bicyclo[3.2.1]oct-3-yl)-amine, 2i

To the mixture obtained from the previous reaction (Procedure 18) was added THF (20 ml_), and the mixture was cooled to -78 ⁰ C. A 1 M solution of lithium aluminum hydride in diethyl ether (60 ml_, 60 mmol) was added in portions. The cooling bath was removed. More THF (60 ml_) and 10 ml_ of the 1 M solution of lithium aluminum hydride were added. The mixture was stirred at rt for 90 min, cooled to -78 ⁰ C, and treated with water (2.6 mL). The cooling bath was removed, and the mixture was allowed to warm to rt. A 1 N NaOH solution (10.6 mL) was added, and the mixture was allowed to stir for 15 min. MgSO ₄ (1g) and THF (40 mL) were added, and the mixite was filtered. To the filtrate was added 2-fluoro-5-bromo-nitrobenzene (5.4 mL, 43.8 mmol), and the mixture was stirred for 15 min. The reaction mixture was filtered, and the solid was washed with THF (3 x 20 mL) and ethyl acetate (4 x 20 mL). The combined filtrates were evaporated, and the residue was purified via flash column chromatography (eluent gradient: 20% EtOAc containing 1% Et ₃ N in heptane to 20% MeOH containing 1% Et ₃ N in EtOAc), yielding 10 g (55.9%) of title compound [2-(4-bromo-2-nitrophenylsulfanyl)-phenyl]-(8-methyl-8-aza- bicyclo[3.2.1]oct-3-yl)-amine, 2i as a mixture of endo and exo isomers.

Procedure 20 3-Bromo- 10-(8-methyl-β-aza-bicyclo[3.2.1]oct-3-yl)- 10W- phenothiazine, 3i

To a solution of [2-(4-bromo-2-nitrophenylsulfanyl)-phenyl]-(8-methyl-8- aza-bicyclo[3.2.1]oct-3-yl)-amine, 2i (9.7 g, 21.6 mmol) in DMSO (220 mL) was added potassium carbonate (3.3 g, 23.8 mmol). The mixture was heated under

a N ₂ atmosphere to 170 ⁰ C for 45 min. The mixture was allowed to cool to rt, diluted with H ₂ O (250 ml_), and extracted with EtOAc (4 x 100 ml_). The organic phase was dried over Na ₂ SO ₄ , filtered, and evaporated. The residue was purified via flash column chromatography (eluent gradient: 1 % Et ₃ N in EtOAc to 30% MeOH in EtOAc containing 1% Et ₃ N), yielding 1.9 g (21.9%) of title compound 3-bromo-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine, 3i as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 401.1/403.1.

Procedure 21 lO^3-Aza-bicyclo[3.2.1]oct-3-yl)-3-bromo- 10H-phenothiazine, 4i

To a solution of 3-bromo-10-t8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine, 3i (1.0 g, 2.49 mmol) in 1 ,2-dichIoroethane (15 mL) were added 1-chloroethyl chloroformate (607 μL, 7.5 mmol) and Λ/,Λ/-diisopropyl-Λ/- ethylamine (1.4 mL, 7.97 mmol). The mixture was heated to reflux for 2.5 h, allowed to cool to rt, and evaporated. The mixture was evaporated and the residue was dissolved dissolved in methanol (15 mL) and heated to reflux for 1 h. After work-up, the residue was purified via flash column chromatography (eluent gradient: 0 to 30% MeOH in EtOAc containing 1% triethylamine) to yield 150 mg of recovered starting material 3i and 3δ2 mg (66%) of title compound 3-bromo-10-piperidin-4-yl-10H-phenothiazine, 4i as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 337.1/369.1.

Procedure 22 3-Bromo- 10-(8-furan-3-ylmethyl-3-aza-bicyclo[3.2.1]oct-3-yl)- 10W- phenothiazine, 5i

To a solution of 3-bromo-10-piperidin-4-yl-10H-phenothiazine, 4i (11.2 mg; 0.029 mmol) and 3-furyl carboxaldehyde (3.4 mg; 0.0δ7 mmol) in dichloroethane (120 μL) was added acetic acid (5 μL) and a solution of sodium triacetoxyborohydride (12 mg, 0.057 mmol) in DMF (100 μ). The mixture was stirred at rt for 1δ h, quenched with water (50 μL), and lyophilized. The thus

obtained crude 3-bromo-10-(8-furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl) - 10H-phenothiazine, 5i as a mixture of endo and exo isomers was used as such for the next reaction.

Procedure 23 lO-t8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyrid in-a-yl- 10H-phenothiazine, 6i

A mixture of 3-bromo-10-(8-furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenothiazine, 5i (13.5 mg, 0.029 mmol), 3-pyridyl boronic acid (10.7 mg, 0.087 mmol), potassium carbonate (12 mg, 0.087 mmol) and Pd(PPh ₃ ) ₄ (3 mg, 2.5 μmol) in NMP (300 μl_) and H ₂ O (100 μL) was heated to 160 ⁰ C for 10 min in a microwave. The mixture was absorbed onto a 1 g SPE cartridge and eluted (eluent: 10% methanol in ethyl acetate containing 1 % triethylamine). The eluent (-15 ml_) was collected and evaporated. The residue was purified via reverse phase HPLC (eluent: acetonitrile in water containing 0.1% TFA) to yield title compound 10-(8-furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3- pyridin-3-yl-10H-phenothiazine, 6i as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 466.2.

W^-tiO^8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10W- phenothiazin-3-yl]-phenyl}-acetamide, 7i

Using an adaptation of the method described in Procedure 23, substituting 2-acetylaminophenyl boronic acid for 3-pyridyl boronic acid, the title compound Λ/-{2-[10-(8-furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl) -1 OH-

phenothiazin-3-yl)-phenyl}-acetamide, 7i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 522.2.

lO-tδ^3-Methyl-but^-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-3-p yridin-3- yl-10/V-phenothiazine, 8i

Using an adaptation of the methods described in Procedures 22 and 23, substituting 3-methyl-but-2-enal for 3-furyl carboxaldehyde in Procedure 22, the title compound 10-[8-(3-methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-3- pyridin-3-yl-10H-phenothiazine, 8i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 454.3.

3-Pyridin-3-yl- 10-(8-pyridin-4-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-

10W-phenothiazine, 9i

Using an adaptation of the methods described in Procedures 22 and 23, substituting 2-pyridyl carboxaldehyde for 3-furyl carboxaldehyde in Procedure 22, the title compound 3-pyridin-3-yl-10-(8-pyridin-2-ylmethyl -8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine, 9i was obtained as a TFA salt and as

a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 477.2.

10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl) -3-pyridin-4- yl-10H-phenothiazine, 10i

Using an adaptation of the methods described in Procedures 22 and 23, substituting 3-methyl-but-2-enal for 3-furyl carboxaldehyde in Procedure 22, and 4-pyridyl boronic acid for 3-pyridyl boronic acid in Procedure 23, the title compound 10-[8-(3-methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-3- pyridin-4- yl-10H-phenothiazine, 1Oi was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 454.3.

3-Pyridin-4-yl- 10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenothiazine, 11 i

Using an adaptation of the methods described in Procedures 22 and 23,

substituting 2-pyridyl carboxaldehyde for 3-furyl carboxaldehyde in Procedure 22, and 4-pyridyl boronic acid for 3-pyridyl boronic acid in Procedure 23, the title compound 3-pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1] oct-3- yl)-10H-phenothiazine, 11i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 477.3.

3-Pyridin^-yl- 10^3-thiophen^-ylmethyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenothiazine, 12i

Using an adaptation of the methods described in Procedures 22 and 23, substituting 2-thiophene carboxaldehyde for 3-furyl carboxaldehyde in Procedure 22, and 4-pyridyl boronic acid for 3-pyridyl boronic acid in Procedure 23, the title compound 3-pyridin-3-yl-10-(8-thiophen-2-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine, 12i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 482.2.

W-ia-Ϊ 10-t8-Allyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenothiazin-3- yl]-phenyl}-acetamide, 13i

Using an adaptation of the methods described in Procedures 22 and 23,

substituting propenal for 3-furyl carboxaldehyde in Procedure 22, and 2- acetylaminophenyl boronic acid for 3-pyridyl boronic acid in Procedure 23, the title compound /V^-fiO^8-allyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiaz in- 3-yl)-phenyl}-acetamide, 13i was obtained as a TFA salt and as a mixture of endo and exo isomers.

ΛH2-[10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazin-3-yl)-phenyl}-acetamide, 14i

Using an adaptation of the methods described in Procedures 22 and 23, substituting phenyl acetaldehyde for 3-furyl carboxaldehyde in Procedure 22, and 2-acetylaminophenyl boronic acid for 3-pyridyl boronic acid in Procedure 23, the title compound N-^-tiO^8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazin-3-yl)-phenyl}-acetamide, 141 was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 546.3.

iV-(2-{10-[8-(1/y-lmiciazol-2-ylmethyl)-8-aza-blcyclo[3.2.1] oct-3-yl)-

10f/-phenothiazin-3-yl}-phenyl)-acetamide, 15i

Using an adaptation of the methods described in Procedures 22 and 23, substituting IH-imidazole-2-carboxaldehyde for 3-furyl carboxaldehyde in Procedure 22, and 2-acetylaminophenyl boronic acid for 3-pyridyl boronic acid in Procedure 23, the title compound Λ/-(2-{10-[8-(1H-lmidazol-2-ylmethyl)-8- aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazin-3-yl)-phenyl)-a cetamide, 15i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 522.3.

Λ/^-tiO-t8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenothiazin-3- yl]-phenyl}-acetamide, 16i

Using an adaptation of the method described in Procedure 23, substituting 3-bromo-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-i OH- phenothiazine, 3i for 3-bromo-10-(8-furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenothiazine, 5i and 2-acetylaminophenyl boronic acid for 3-pyridyl boronic acid, the title compound Λ/-{2-[10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenothiazin-3-yl)-phenyl}-acetamide, 16i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 456.2.

lO^3-Aza-bicyclo[3.2.1]oct-3-ylJ-3-pyridin-3-yl- 10H-phenothiazine,

17i

Using an adaptation of the method described in Procedure 23, substituting 3-bromo-10-piperidin~4-yl-10/7-phenothiazine, 4i for 3-bromo-10-(8- furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothia zine, 5i, the title compound 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-phenoth iazine, 17i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 386.2.

W-{2-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10W-phenothiazin- 3-yl)- phenyl}-acetamide, 18i

Using an adaptation of the method described in Procedure 23, substituting 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-bromo-10H-phenothiazine, 4i for 3-bromo-10-(8-furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl) -10H- phenothiazine, 5ϊ and 2-acetylaminophenyl boronic acid for 3-pyridyl boronic acid, the title compound Λ/-{2-[10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazin-3-yl)-phenyl}-acetamide, 18i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 442.2.

lO-t8-Allyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-

phenothiazine, 19i Using an adaptation of the methods described in

Procedures 22 and 23, substituting 2-propenal for 3-furyl carboxaldehyde in

Procedure 22, the title compound 10-(8-alIyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3- pyridin-3-yl-10H-phenothiazine, 19i was obtained as a TFA salt and as a mixture of endo and exo isomers. MS m/z (M+18+H) 444.2.

Endo-3-(4-Chloro-2-hydroxyphenylamino)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1j and exo-3-(4- chloro-2-hydroxyphenyl-amino)-8-aza-bicycIo[3.2.1]octane-8-c arboxylic

acid tert-butyl ester, 2j

Using an adaptation of the method described in Procedure 1 , substituting 2-amino-5-chlorophenol for 4-amino-3-hydroxybenzoic acid methyl ester and 3-oxo-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester for 8~methyl-8~aza-bicyclo[3.2.1]octan-3-one, a 3:1 mixture of title compounds endo-3-(4-chloro-2-hydroxyphenylamino)-8-aza-bicyclo[3.2.1]o ctane-8- carboxylic acid tert-butyl ester, 1j and exo-3-(4-chloro-2-hydroxyphenyl-amino)- 8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2j was obtained. Compounds 1j and 2j were separated via flash column chromatography (eluent gradient: 3% to 65% EtOAc in hexane), yielding 70% of endo isomer 1j (first eluting isomer; and 19% of exo isomer 2j (second eluting isomer)

Endo-3-(3-Chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1]oct ane-8- carboxylic acid tert-butyl ester, 3j

Using an adaptation of the method described in Procedure 2, substituting enc/o-3-(4-chloro-2-hydroxyphenyIamino)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1j for the TFA salt of 3- hydroxy-4-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)-benzo ic acid methyl ester, 1a, the title compound eπc/o-3-(3-chlorophenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3j was obtained.

Procedure 24

Endo-3-(3-Pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j

To a mixture of 3-pyridyl boronic acid (70.1 mg, 0.57 mmol), Cp ₂ Fe(P- tBu2) 2PdCI ₂ (62 mg, 0.095 mmol), potassium carbonate (88 mg, 0.63 mmol) was added a solution of e/π/o-3-(3-chlorophenoxazin-10-yl)-8-aza- bicydo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3j (135 mg, 0.317 mmol) in dioxane (3 ml_, degassed with argon for 5 min prior to use). The mixture was heated to 120 ⁰ C for 30 min in a microwave (300 W). The mixture was allowed to cool to rt, water and ethyl acetate were added. The organic layer was separated, dried over MgSO4, filtered, and evaporated to yield 195 mg of crude

title compound enc/o-3-(3~pyridin-3~yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j. The material was used as such for the next reaction.

Procedure 25

Enc/o-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10 H- phenoxazine, 5j

The crude e/?cfo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j, obtained from the previous reaction, was treated with TFA (3 ml_), and the mixture was stirred for 30 min at rt. The TFA was removed under a nitrogen stream, and the residue was purified via reverse phase HPLC (eluent gradient: 10% to 30% CH ₃ CN in water containing 0.1% TFA). The desired fractions were combined, lyophilized, and dissolved in diethyl ether. The solution was washed with a saturated NaHCO ₃ solution. The aqueous phase was washed with diethyl ether, and the combined organic phases were dried over MgSO ₄ , filtered, and evaporated to yield 58 mg (43% for 2 steps) of title compound endo-10-(8-aza- bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-phenoxazine, 5j. MS m/z (MH ⁺ ) 370.2.

Enc/o-10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyr idin-3-yl)-

10H-phenoxazine, 6j

Using an adaptation of the method described in Procedure 9, substituting eπdo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H- phenoxazine, 5j for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)- 3-Ory- tetrazol-5-yl)-10H-phenoxazine, 4b and phenyl acetaldehyde for 2- pyridylcarboxaldehyde, the title compound e/ido-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-phenoxazine, 6j was obtained as a TFA salt. MS m/z (MH ⁺ ) 474.2.

Endo-3-Pyridin-3-yl- 10-t8-pyridin^-ylmethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazine, 7j

Using an adaptation of the method described in Procedure 9, substituting endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H- phenoxazine, 5j for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H- tetrazol-5-yl)-10H-phenoxazine, 4b and 2-pyridyl carboxaldehyde for 2- pyridylcarboxaldehyde, the title compound endo-3-pyridin-3-yl~10-(8-pyridin-2- ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine, 7j was obtained as a TFA salt. MS m/z (MH ⁺ ) 461.2.

Endo- 10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyrid in- 3-yl-10H-phenoxazine, δj

Using an adaptation of the method described in Procedure 9, substituting encfo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-1 OH- phenoxazine, 5j for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H- tetrazol-5-yl)-10H-phenoxazine, 4b and 3-furaldehyde for 2- pyridylcarboxaldehyde, the title compound endo-10-(8-furan-3-ylmethyl-8-aza-

bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-phenoxazine, 8j was obtained as a TFA salt. MS m/z (MH ⁺ ) 450.2.

E/7do-3-Pyridin-3-yl-10-(8-thiophen-2-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine, 9j Using an adaptation of the method described in Procedure 9, substituting endo-10-(8-aza- bicyciotS^.iloct-3-yl)-3-pyridin-3-yl-10H-phenoxazine, 5j for the HCI salt of 10- (8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)-10H-phen oxazine, 4b and 2- thiophene carboxaldehyde for 2-pyridylcarboxaldehyde, the title compound eπc/o-3-pyridin-3-yl-10-(8-thiophen-2-ylmethyl-8-aza-bicycl o[3.2.1]oct-3-yl)-10H- phenoxazine, 9j was obtained as a TFA salt. MS m/z (MH ⁺ ) 466.2.

£nc/o-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-chloro-10H-phe noxazine,

10j

Using an adaptation of the method described in Procedure 25, substituting enc/o-3-(3-chloro-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octa ne-8- carboxylic acid tert-butyl ester, 3j for endo-3-(3-pyridin-3-yl-phenoxazin-10-yl)- 8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j, the title compound eπdo-10-(8-aza-bicyclo[3.2.1]-oct-3-yl)-3-chloro-10H-phenox azine, 1Oj was obtained as a TFA salt after reverse phase HPLC purification (eluent:

CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 327.1.

EXAMPLE K

Endo- 3-(3-Pyridin-4-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1k

Using an adaptation of the method described in Procedure 24, substituting 4-pyridyl boronic acid for 3-pyridyl boronic acid, the title compound enQfo-3^ 3-pyridin-3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane- 8- carboxylic acid tert-butyl ester, 1k was obtained.

£nc/o-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-4-yl-1 0H- phenoxazine, 2k

Using an adaptation of the method described in Procedure 25, substituting encfo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1k for enc/o-3-(3-pyridin- 3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]-octane-8-carboxy lic acid tert-butyl

ester, 4j, the title compound enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-4- yl-10H-phenoxazine, 2k was obtained as a TFA salt after reverse phase HPLC purification (eluent gradient: 10% to 30% CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 370.2.

Endo- 10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-Φyl - 10H-phenoxazine, 3k

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-4-yl- 10H~phenoxazine, 2k for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H- tetrazol-5-yl)-10H-phenoxazine, 4b and phenyl acetaldehyde for 2-pyridyl carboxaldehyde, the title compound endo-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-phenoxazine, 3k was obtained as a TFA salt. MS m/z (MH ⁺ ) 474.3.

£nc/o-3-Pyridin-4-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicy clo[3.2.1]oct- 3-yl)-10H-phenoxazine, 4k

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-phenoxazine, 2k for the HCI salt of 10-t8-aza-bicyclo[3.2.1]oct-3-yl)-3-OH- tetrazol-5-yl)-10H-phenoxazine, 4b, the title compound enc/o-3-pyridin-3-yl-10- (8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phen oxazine, 4k was obtained as a TFA salt. MS m/z (MH ⁺ ) 461.3.

Endo- 10^β-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyrid in- 4-yl-10H-phenoxazine, 5k

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-phenoxazine, 2k for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yI)~3-(1H- tetrazol-5-yl)-10H-phenoxazine, 4b and 3-furaldehyde for 2- pyridylcarboxaldehyde, the title compound eπcfo-10-(8-furan-3-ylmethyl-8-aza- bicydotS^.iloct-3-yl)-3-pyridin-4-yl-10H-phenoxazine, 5k was obtained as a TFA salt. MS m/z (MH ⁺ ) 450.3.

Endo-3-Pyridin-4-yl-10-(8«thiophen-2-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10W-phenoxazine, 6k

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-phenoxazine, 2k for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H- tetrazol-5-yl)-10H-phenoxazine, 4b and 2-thiophene carboxaldehyde for 2- pyridylcarboxaldehyde, the title compound enc/o-3-pyridin-3-yl-10-(8-thiophen- 2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine, 6k was obtained as

a TFA salt. MS m/z (MH ⁺ ) 466.2.

EXAMPLE L

£xo-3-(3-Chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1]oct ane-8- carboxylic acid tert-butyl ester, 11

Using an adaptation of the method described in Procedure 2, substituting exo-3-(4-chloro-2-hydroxyphenylamino)-8-aza-bicyclo[3.2.1]oc tane- 8-carboxylic acid tert-butyl ester, 2j for the TFA salt of 3-hydroxy-4-(8-methyI-8- aza-bicyclo[3.2.1]oct-3-ylamino)-benzoic acid methyl ester, 1a, the title compound exo-3-(3-chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane~ 8- carboxylic acid tert-butyl ester, 11 was obtained.

Endo- 3-(3-Pyridin-4-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2I

Using an adaptation of the method described in Procedure 24, substituting exo-3-(3-chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane- 8- carboxylic acid tert-butyl ester, 11 for enc/o-3-(3~chlorophenoxazin-10~yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3j, the title compound 3- (3-pyridin-3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane -8-carboxylic acid tert-butyl ester, 2I was obtained.

Exo-S^3-Pyridin-3-yl-phenoxazin- 10-yl)-8-aza-bicyclo[3.2.1]octane-

8-carboxylϊc acid tert-butyl ester, 31 Using an adaptation of the method described in Procedure 25, substituting exo-3-(3-pyridin-3-yI-phenoxazin-10-yl)-

8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2I for endo-3-(3- pyridin-3-yI-phenoxazin-10-yl)-8-aza-bicycIo[3.2.1]-octane-8 -carboxylic acid tert-butyl ester, 4j, the title compound exo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-

8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3I was obtained as a TFA salt after reverse phase HPLC purification (eluent gradient: 10% to 30%

CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 370.2.

Exo- 10-t8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-ylJ-3-pyridin-3-yl - 10H- phenoxazine, 41

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of exo-3-(3-pyridin-3-yl-phenoxazin-10-yl)~8-aza~ bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3I for the HCI salt of 10- (8-aza-bicyclo[3.2.1]oct-3-yl)-3^IH-tetrazol-8-yl)-10H-pheno xazine, 4b and phenyl acetaldehyde for 2-pyridyl carboxaldehyde, the title compound exo-10- (8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10 H-phenoxazine, 41 was obtained as a TFA salt. MS m/z (MH ⁺ ) 474.3.

Ejfo-3-Pyridin-3-yl- 10^3-pyridin-4-ylmethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10W-phenoxazine, 5I

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of exo~3-(3-pyridin-3-yl-phenoxazin-10~yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3I for the HCI salt of 10- (8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)-10H-phen oxazine, 4b, the title compound exo-3-pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3. 2.1]oct-3- yl)-10H-phenoxazine, 5I was obtained as a TFA salt. MS m/z (MH ⁺ ) 461.3.

Exo- 10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyrid ϊn-3- yMOW-phenoxazine, 6I

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of exo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 31 for the HCI salt of 10- (8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)-10H-phen oxazine, 4b and 3- furaldehyde for 2-pyridyl carboxaldehyde, the title compound exo-10-(8-furan-3- ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-ph enoxazine, 6I was

obtained as a TFA salt. MS m/z (MH ⁺ ) 450.2.

£xo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-chloro-10H-pheno xazine, 7I

Using an adaptation of the method described in Procedure 25, substituting exo-3-(3-chloro-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane -8- carboxylic acid tert-butyl ester, 11 for enc/o-3-(3-pyridin-3-yl-phenoxazin-10-yl)- 8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j, the title compound exo-10-(8-aza-bicyclo[3.2.1]-oct-3-yl)-3-chloro-10H-phenoxaz ine, 7I was obtained as a TFA salt after reverse phase HPLC purification (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 327.1.

EXAMPLE M

Exo- 3-t3-Pyridin^-yl-phenoxazin- 10-yl)-8-aza-bicyclo[3.2.1]octane- 8-carboxylic acid tert-butyl ester, 1m

Using an adaptation of the method described in Procedure 24, substituting 4-pyridyl boronic acid for 3-pyridyl boronic acid, the title compound exo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1] octane-8-carboxylic acid tert-butyl ester, 1m was obtained.

Exo- 10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-^yl- 10H- phenoxazine, 2m

Using an adaptation of the method described in Procedure 25, substituting exo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1] octane- 8-carboxylic acid tert-butyl ester, 11 for er?c/o-3-(3-pyridin-3-yl-phenoxazin-10- yl)-8-aza-bicyclo[3.2.1]-octane-8-carboxylic acid tert-butyl ester, 4j, the title

compound exo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H- phenoxazine, 2m was obtained as a TFA salt after reverse phase HPLC purification (eluent gradient: 10% to 30% CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 370.2.

Exo-10^ 3-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-^yl-10H- phenoxazine, 3m

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of exo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-phenoxazine, 2m for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3- (1H-tetrazol-5-yl)-10H-phenoxazine, 4b and phenyl acetaldehyde for 2-pyridyl carboxaldehyde, the title compound exo-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10/7-phenoxazine, 3m was obtained as a TFA salt. MS m/z (MH ⁺ ) 474.3.

Exo-3-Pyridin^-yl-10^ 3-pyridin^-ylmethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazine, 4m

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of exo-10~(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-phenoxazine, 2m for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3- (1H-tetrazol-5-yl)-10H-phenoxazine, 4b, the title compound exo-3-pyridin-3-yl- 10-(8-pyridin-2-ylmethyl -8-aza-bicyclo[3.2.1]oct-3-yl)-i 0H-phenoxazine, 4m was obtained as a TFA salt. MS m/z (MH ⁺ ) 461.2.

Exo- 10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-a-pyrid in-4- yl-10H-phenoxazine, 5m

Using an adaptation of the method described in Procedure 9, substituting the TFA salt of exo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl- 10H-phenoxazine, 2m for the HCI salt of 10-(8-aza-bicyclo[3.2.1]oct-3~yl)-3- (1H-tetrazol-5-yl)-10H-phenoxazine, 4b and 3-furaldehyde for 2-pyridyl carboxaldehyde, the title compound exo-10-(8-furan-3-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-3-pyridin-3-yl-10H-phenoxazine, 5m was obtained as a TFA salt. MS m/z (MH ⁺ ) 450.2.

EXAMPLE N

£xo- 3-(3-Cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-

carboxylic acid tert-butyl ester, 1n

Using an adaptation of the method described in Procedure 6, substituting exo-3-^-hydroxy-phenylaminoJ-8-aza-bicyclo[3.2.1]octane-8- carboxylic acid tert-butyl ester, 2b for e/7do-3-(2-hydroxy-phenylamino)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1b, the title compound exo- 3-(3-cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-ca rboxylic acid tert-butyl ester, 1n was obtained. MS m/z (MH ⁺ ) 439.9.

Exo-3-[3-(1 f/-Tetrazol-5-yl)-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2n

Using an adaptation of the method described in Procedure 7, substituting exo-3-(3-cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane- 8- carboxylic acid tert-butyl ester, 1n for endo-3-(3-cyano-phenoxazin-10-yl)-8- aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3b, the title compound exo-3~[3-(1 H-tetrazol-5-yl)-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2n was obtained. MS m/z (MH ⁺ ) 460.9.

Exo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-1 OW- phenoxazine, 3n Using an adaptation of the method described in Procedure 8, substituting exo-3-[3-(1 H-tetrazol-5-yl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]- octane-8-carboxyIic acid tert-butyl ester, 2n for enc/o-3-[3-(1H-tetrazol-5-yl)- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]-octane-8-carboxylic acid tert-butyl ester,

4b, the title compound exo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5- yl)-10H-phenoxazine, 3n was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z

(MH ⁺ ) 360.9.

Exo- 10-(8-Aza-bicyclo[3.2.1]oct-3-yl)- 10W-phenoxazine-3-

carbonitrile, 4n

Using an adaptation of the method described in Procedure 25, substituting exo- 3-(3-cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8- carboxylic acid tert-butyl ester, 1n for endo-3-(3-pyridin-3-yl-phenoxazin-10-yl)- 8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j, and using a 1:1 solution of TFAjCH ₂ Cb instead of neat TFA, the title compound exo-10-(8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbonitrile, 4n was obtained as a TFA salt after reverse phase HPLC purification (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 317.9.

EXAMPLE O

Exo-3-[3-(Λ^Hydroxycarbamϊmidoyl)φhenoxazin-10-yl)-8-a za- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1o

Using an adaptation of the method described in Procedure 11 , substituting exo-3-(3-cyano-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane- 8- carboxylic acid tert-butyl ester, 1n for enc/o-3-(3-cyano-phenoxazin-10-yl)-8- aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3b, the title compound θxo-3-[3-(Λ/-hydroxycarbamimidoyl)-phenoxazin-10-yl)-8-aza - bicydo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1o was obtained. MS

m/z (MH ⁺ ) 450.9.

£xo-3-[3-(5-Oxo-4,5-dihydro-[1,2,4]oxadiazol-3-yl)-pheno xazin-10- yl]-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2o

Using an adaptation of the method described in Procedure 12, substituting exo-3-[3-(Λ/-hydroxycarbamimidoyl)-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1o for the mixture of eπc/o-3-[3-(/V-hydroxycarbamimidoyl)-phenoxazin-10-yl)-8-az a- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1d and encfo-3-(3- carbamoyl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-car boxylic acid tert- butyl ester, 2d, the title compound exo-3~[3-(5~oxo-4,5-dihydro~[1 ,2,4]oxadiazol- 3-yl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxy lic acid tert-butyl ester, 2o was obtained.

£xo-3-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin- 3-yl)-4H-

[1,2,4]oxadiazol-5-one, 3o

Using an adaptation of Procedure 8, and substituting exo-3-[3-(5-oxo-4,5- dihydro-ti ^^loxadiazol-3-yl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]oct ane-8- carboxylic acid tert-butyl ester, 2o for enc/o-3-[3-(1H-tetrazol-5-yl)-phenoxazin- 10-yl)-8-aza-bicyclo[3.2.1]-octane-8-carboxylic acid tert-butyl ester, 4b, the title compound exo-3-[10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3-yl )-4H- [1 ,2,4]oxadiazol-5-one, 3o was obtained as TFA salt after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 376.8.

EXAMPLE P

3-Methoxy^^8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)- benzonitrile, 1p

Using an adaptation of Procedure 2, substituting 8-methyl-8-aza- bicyclo[3.2.1]oct-3-ylamine for the TFA salt of 3-hydroxy-4-(8-methyl-8-aza- bicyclo[3.2.1]oct-3-ylamino)-benzoic acid methyl ester, 1a, 1-fluoro-3-methoxy- 2-nitrobenzene, 1e for 2-fluoronitrobenzene, and cesium carbonate for potassium carbonate, the title compound 3-methoxy-4-(8-methyl-8-aza- bicyclo[3.2.1]oct-3-ylamino)-benzonitrile, 1p was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1% TFA). MS m/z= 272 (M+1).

3-Hydroxy-4-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)- benzonitrile, 2p

Using an adaptation of Procedure 13, substituting the TFA salt of 3- methoxy^8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino^benzonitr ile, 1p for the TFA salt of e-methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-

phenoxazine-3-carboxylic acid diethylamide, 6e, the title compound 3-hydroxy- 4-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)-benzonitrile, 2p was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1% TFA). MS m/z= 258 (M+1).

θ-Methoxy- 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H- phenoxazine-3-carbonitrile, 3p

Using an adaptation of Procedure 2, substituting the TFA salt of 3- hydroxy-4-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)-benzo nitrile, 2p for the TFA salt of 3-hydroxy-4-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-ylamino)-ben zoic acid methyl ester, 1a, and 1-fluoro-3-methoxy~2-nitrobenzene, 1e for 2- fluoronitrobenzene, the title compound 6-methoxy~10-(8-methyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carbonitrile, 3p was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1% TFA). MS m/z= 362 (M+1).

e-Hydroxy- 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl^iOH- phenoxazine-3-carbonitrile, 4p

Using an adaptation of Procedure 13, substituting the TFA salt of 6- methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno xazine-3- carbonitrile, 3p for the TFA salt of 6-methoxy-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 6e, the title compound 6-hydroxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenoxazine-3-carbonitrile, 4p was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1% TFA). MS m/z= 348 (M+1).

EXAMPLE Q

THF

Procedure 26

[10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenothiazin-3-yl]-pyrrolidin-1-yl-methanone, 1q

To a solution of a mixture of endo and exo isomers of 3-bromo-10- piperidin-4-yl-10H-phenothiazine, 4i (15 mg, 0.04 mmol) in dichloroethane (0.4 ml_) were added 3-furaldehyde (10 μl_, 0.12 mmol), acetic acid (5 μl_) and sodium triacetoxy-borohydride (17 mg, 0.08 mmol). The mixture was allowed to stir at rt for 16 h, and quenched with a 2N NaOH solution (200 μl_). The mixture was absorbed onto a 1 g SPE cartridge and eluted (eluent: 10% methanol in ethyl acetate containing 1 % triethylamine). The eluent (~15 ml_) was collected and evaporated. The residue was dissolved in THF (0.4 ml_), and pyrrolidine (19 μl_, 0.15 mmol), Mo(CO) ₆ (16 mg, 0.06 mmol), Herrmann's catalyst (6 mg, 0.006 mmol), and DBU (27 μl_, 0.18 mmol) were added. The mixture was irradiated in a microwave oven at 150 ⁰ C for 15 min. The mixture was evaporated, and the residue was purified via reverse phase HPLC (eluent gradient: CH ₃ CN in water containing 0.1% TFA) to yield [10-(8-furan-3- ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazin-3-yl )-pyrrolidin-i-yl- methanone, 1q as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 486.2.

[lO-t8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenothiazin-3- yl]-pyrrolidin-1-yl-methanone, 2q

Using an adaptation of Procedure 26, substituting phenyl acetaldehyde for 3-furaldehyde, the title compound [10-(8-phenethyl~8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenothiazin-3-yl)-pyrrolidin-1-yl-methanone, 2q was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1 % TFA). MS m/z= 510.3 (M+1 ).

{10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl ]-10H- phenothiazin-3-yl}-pyrrolidin-1-yl-methanone, 3q

Using an adaptation of Procedure 26, substituting 3-methyl-but-2-enal for 3-furaldehyde, the title compound {10-[8-(3-methyl-but-2-enyl)-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenothiazin-3-yl)-pyrrolidin-1 -yl-methanone, 3q was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1 % TFA). MS m/z= 474.2 (M+1 ).

[10-(8-Furan-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenothiazin-3-yl]-(3-hydroxy-pyrrolidin-1 -yl)-nr»ethanone, 4q

Using an adaptation of Procedure 26, substituting 3-hydroxypyrrolidine for pyrrolidine, the title compound [10-(8-furan-3-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenothiazin-3-y^^ 3-hydroxy-pyrrolidin-i-yl)- methanone, 4q was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1 % TFA). MS m/z= 502.2 (M+1 ).

(3-Hydroxy-pyrrolidϊn-i-yl)-I 10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenothiazin-3-yl)-methanone, 5q

Using an adaptation of Procedure 26, substituting phenyl acetaldehyde for 3-furaldehyde and 3-hydroxypyrrolidine for pyrrolidine, the title compound ( 3-hydroxy-pyrrolidin-i-yl)-tiO-C8-phenethyl-8-aza-bicyclo[3. 2.1]oct-3-yl)-10H- phenothiazin-3-yl)-methanone, 5q was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent

gradient: 20% to 45% CH ₃ CN in water containing 0.1 % TFA). MS m/z= 526.2 (M+1 ).

{lO-tδ^3-Methyl-but-a-enylJ-8-aza-bicyclo[3.2.1]oct-3-yl )- 10H- phenothiazϊn-3-yl}-(3-methyl-pyrrolidin-1-yl)-methanone, 6q

Using an adaptation of Procedure 26, substituting 3-methyl-but-2-enal for 3-furaldehyde and 3-hydroxypyrrolidine for pyrrolidine, the title compound {10-[8-(3-methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-1 0H-phenothiazin-3- yl}-(3-methyl-pyrrolidin-1-yl)-methanone, 6q was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1% TFA). MS m/z= 490.2 (M+1 ).

EXAMPLE R

Procedure 27

[lO-t8-Methyl-8-aza-bicyclo[3.2.1]oct-S-yl)- 10H-phenothiazin-3-yl]- pyrrolidin-1-yl-methanone, 1r

To a solution of 3-bromo-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine, 3i (15 mg, 0.04 mmol) in THF (0.3 mL) was added pyrrolidine

(12 μl_, 0.15 mmol), Mo(CO) ₆ (16 mg, 0.06 mmol), Herrmann's catalyst (6 mg, 0.006 mmol), and DBU (27 μl_, 0.18 mmol), and the mixture was irradiated in a microwave oven at 150 ⁰ C for 15 min. The mixture was evaporated, and the residue was purified via reverse phase HPLC (eluent gradient: CH ₃ CN in water containing 0.1% TFA) to yield [10-t8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazin-3-yl)-pyrrolidin-1-yl-methanone, 1r as a TFA salt and a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 420.2.

(3-Hydroxy-pyrrolidin-i-yl)-I 10-t8-methyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenothiazin-3-yl)-methanone, 2r

Using an adaptation of Procedure 27, substituting 3-hydroxypyrrolidine for pyrrolidine, the title compound (3-hydroxy-pyrrolidin-1-yl)-[10-(8-methyl-8- aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazin-3-yl)-methanone , 2rwas obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1 % TFA). MS m/z= 436.2 (M+1 ).

lO-t8-Methyl-8-aza-bicyclo[3.2.1]oct-S-yl)- 10H-phenothiazine-3- carboxylic acid ethylamide, 3r

Using an adaptation of Procedure 27, substituting ethylamine for

pyrrolidine, the title compound 10-(8-methyl-8-aza~bicyclo[3.2.1]oct-3~yl)-10H- phenothiazine-3-carboxylic acid ethylamide, 3r was obtained as TFA salt and as a mixture of endo and exo isomers after purification via reverse phase HPLC (eluent gradient: 20% to 45% CH ₃ CN in water containing 0.1% TFA). MS m/z= 394.2 (M+1 ).

EXAMPLE T

£/7do-3-(3-Diethylcarbamoyl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1t

Using an adaptation of Procedure 6, and substituting eπdo-3-(2-hydroxy- phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1b for 3-(2-hydroxy-phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carbo xylic acid tert-butyl ester, 1b, the title compound enc/o-3-(3-diethylcarbamoyl-phenoxazin- 10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1t was obtained. The crude material was used as such in the next reaction.

Enc/o-10-(8-Aza-bicycIoIS^.Iloct-3-yl^iOH-phenoxazine-3- carboxylic acid diethylamide, 2t

Using an adaptation of Procedure 25, substituting endo-3-(3- diethylcarbamoyl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octan e-8-carboxylic acid tert-butyl ester, 1t for encfo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane~8-carboxylic acid tert-butyl ester, 4j, and using a 25% solution of TFA in methylene chloride instead of neat TFA, the title compound endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-car boxylic acid diethylamide, 2t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 392.1.

Endo-Λ O-f8-Thiophen-a-ylmethyl-8-aza-bicyclo[3.2.1]oct-a-yl)- i OH- phenoxazine-3-carboxylic acid diethylamide, 3t

Using an adaptation of Procedure 9, and substituting enc/o-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2t for enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H-phenoxazine, 5b, thiophene-2-carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound endo-10-(8- thiophen-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenox azine-3-carboxylic acid diethylamide, 3t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 488.1.

Enc/o-10-(8-Pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl) -10W- phenoxazine-3-carboxylic acid diethylamide, 4t

Using an adaptation of Procedure 9, and substituting er?c/o- 10-(8-aza- bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxylic acid diethylamide, 2t for er?c/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl )-10H-phenoxazine, 5b, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound eπdo-1 O^8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-i OH- phenoxazine-3-carboxylic acid diethylamide, 4t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 483.1.

Endo- 10-(8-Thiazol^-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10W- phenoxazine-3-carboxylic acid diethylamide, 5t

Using an adaptation of Procedure 9, and substituting encfo-10-(8-aza- bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxylic acid diethylamide, 2t for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)- 10H-phenoxazine, 5b, thiazoI-2-ylmethyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound e/7c/o-10-(8-thiazol- 2~ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH-phenoxazine-3-carboxylic acid diethylamide, 5t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 489.1.

Endo-Λ 0-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenoxazϊne-3-carboxylic acid diethylamide, 6t

Using an adaptation of Procedure 9, and substituting enαO-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2t for eπc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl )-10H-phenoxazine, 5b, phenyl acetaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxy-borohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloro-ethane, the title compound eπoO-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine~3-carboxylic acid diethylamide, 6t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA).

Endo-Λ 0-(8-Pyridin-3-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenoxazine-3-carboxylic acid diethylamide, 7t

Using an adaptation of Procedure 9, and substituting enαto-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3~carboxylic acid diethylamide, 2t for encfo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-10H-phenoxazine,

5b, 3-pyridyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxy-boro hydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloro-ethane, the title compound enc/o-10-(8-pyridin- 3-ylmethyI-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-c arboxylic acid diethylamide, 7t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 483.1.

Endo-IO-fδ^S-Methyl-but^-enylJ-δ-aza-bicyclo[3.2.1]oct- 3-yl)-IOH- phenoxazine-3-carboxylic acid diethylamide

Using an adaptation of Procedure 9, and substituting endo- 10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2t for endo-λ 0-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H~tetrazol-5-yl)-10H-phenoxazine, 5b, 3-methyl-but-2-enyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound eπdo-10-[8-(3- methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxa zine-3-carboxylic acid diethylamide, 8t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 460.1.

Endo- 10-I3-tiH-lmidazol-a-ylmethyl)-8-aza-bicyclo[3.2.1]oct-3-yl) - 10H-phenoxazine-3-carboxylic acid diethylamide, 9t

Using an adaptation of Procedure 9, and substituting eπc/o-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2t for enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl) -10H-phenoxazine, 5b, 1H-imidazol-2-ylmethyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound eπdo-10-[8-(1H- imidazol-2-ylmethyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno xazine-3- carboxylic acid diethylamide, 9t was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 472.1.

Endo- 10-(8-Benzyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3- carboxylic acid diethylamide, 10t

Using an adaptation of Procedure 9, and substituting endo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2t for eπdo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazoi-5-yl) -10H-phenoxazine,

5b, benzaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyboro hydride, and tetrahydrofuran for dichloroethane, the title compound e/7c/o-10-(8-benzyl-8-aza~bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 1Ot was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 482.1.

EXAMPLE U

Exo-3-(3-Diethylcarbamoyl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1u

Using an adaptation of Procedure 6, and substituting exo-3-(2-hydroxy- phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2b for 3-(2-hydroxy-phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carbo xylic acid tert-butyl ester, 1b, the title compound exo-3-(3-diethylcarbamoyl-phenoxazin- 10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1u was obtained. The crude material was used as such in the next reaction.

Exo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carb oxylic acid diethylamide, 2u

Using an adaptation of Procedure 25, substituting exo-3-(3- diethylcarbamoyl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octan e-8-carboxylic acid tert-butyl ester, 1u for enc/o-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j, and using a 25% solution of TFA in methylene chloride instead of neat TFA, the title compound exo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-carb oxylic acid diethylamide, 2u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 392.1.

Exo- 10-(8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10W-phenoxazine- 3-carboxylic acid diethylamide, 3u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2u for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-1 OH-phenoxazine, 5b, phenyl acetaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxy-borohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloro-ethane, the title compound exo-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3~carboxylic acid diethylamide, 3u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ )

Exo-10-f8-Pyridin-a-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-ylH OH- phenoxazine-3-carboxylic acid diethylamide, 4u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3~yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2u for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-1 OH-phenoxazine, 5b, 3-pyridyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxy-borohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloro-ethane, the title compound exo-10-(8-py rid i n-3- ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-car boxylic acid diethylamide, 4u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 483.1.

Exo- 10-(8-Thiophen-a-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-ylJ- 10W- phenoxazine-3-carboxylic acid diethylamide, 5u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2u for eπofo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl )-10H-phenoxazine, 5b, thiophene-2-carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound exo-10-(8-thiophen- 2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH-phenoxazine-3-carboxylic acid diethylamide, 5u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 488.1.

Exo-10-[8-(3-Methyl-but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3 -yl]-10H- phenoxazine-3-carboxylic acid diethylamide, 6u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxylic acid diethylamide, 2u for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-1 OH-phenoxazine, 5b, 3-methyl-but-2-enyl carboxaldehyde for 2-pyridyI carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound exo-10-[8-(3-methyl- but-2-enyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3- carboxylic acid diethylamide, 6u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 460.1.

Exo-IO-tδ-Pyridin^-ylmethyl-δ-aza-bicyclo[3.2.1]oct-3-y lJ-IOH- phenoxazine-3-carboxylic acid diethylamide, 7u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxylic acid diethylamide, 2u for

enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1r7-tetrazol-5-yl )-10H-phenoxazine, 5b, tθtrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound exo-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 0/-y- phenoxazine-3-carboxylic acid diethylamide, 7u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS /77/Z (MH ⁺ ) 483.1.

£xo-10-(8-Benzyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno xazine-3- carboxylic acid diethylamide, 8u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxylic acid diethylamide, 2u for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)- 10H-phenoxazine, 5b, benzaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound exo-10-(8-benzyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 8u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 482.1.

£xo-10-(8-ThiazoI-2-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-y l)-10W- phenoxazine-3-carboxylic acid diethylamide, 9u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2u for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)- 10H-phenoxazine, 5b, thiazol-2-ylmethyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound exo-10-(8-thiazol-2- ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine-3-car boxylic acid diethylamide, 9u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 489.1.

Exo- 10-(8-Furan-3-ylmethyl-β-aza-bicyclo[3.2.1]oct-3-yl)- 10H- phenoxazine-3-carboxylic acid diethylamide, 10u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxylic acid diethylamide, 2u for

enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl) -10H-phenoxazine, 5b, thiazol-2-ylmethyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound exo-10-(8-furan-3- ylmethyl-8-aza-bicyclo[3.2.1]-oct-3-yl)-10H-phenoxazine-3-ca rboxylic acid diethylamide, 1Ou was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 472.1.

Exo- 10-ϊ8-tiH-lmidazol-a-ylmethylJ-8-aza-bicyclo[3.2.1]oct-3-yl ]- 10H-phenoxazine-3-carboxylic acid diethylamide, 11u

Using an adaptation of Procedure 9, and substituting exo-10-(8-aza- bicyclo-[3.2.1]oct-3-yl)-10H-phenoxazine-3-carboxy!ic acid diethylamide, 2u for endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3^IH-tetrazol-8-yl)-1 0H-phenoxazine, 5b, 1H-imidazol-2-ylmethyl carboxaldehyde for 2-pyridyl carboxaldehyde, tetrabutylammonium triacetoxyborohydride for sodium triacetoxyborohydride, and tetrahydrofuran for dichloroethane, the title compound xxo-10-[8-(1H- imidazol-2-ylmethyl)-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-pheno xazine-3- carboxylic acid diethylamide, 11u was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 472.1.

EXAMPLE V

Endo-3-(3-Chloro-6-methoxy-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1v

Using an adaptation of Procedure 2, and substituting 6-fluoro-2- methoxy-nitrobenzene, 1e for 4-fluoro-3-nitrobenzonitrile and encfo-3-(4-ch!oro- 2-hydroxy-phenylamino)-8-aza-bicyclo[3.2.1]octane-8-carboxyl ic acid tert-butyl ester, 1j for the TFA salt of 3-hydroxy-4-(8-methyl-8-aza-bicyclo[3.2.1]oct-3- ylamino)-benzoic acid methyl ester, 1a, the title compound er?oto-3-(3-chloro-6- methoxy-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carbo xylic acid tert- butyl ester, 1v was obtained. MS m/z (MH ⁺ ) 457.2.

£/7do-3-(6-Methoxy-3-pyridin-3-yl-phenoxazin-10-yl)-8-az a- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2v

Using an adaptation of the method described in Procedure 24, substituting enc/o-3-(3-chloro-6-methoxy-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1v for encfo-3-(3- chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1]-octane-8-carbox ylic acid tert-butyl ester, 3j, the title compound encfo-3-(6-methoxy-3-pyridin-3~yl-phenoxazin-10- yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2v was obtained. MS m/z (MH ⁺ ) 500.3.

Endo- 10-(8-Aza-bicyclo[3.2.1]oct-3-ylH-pyridin-3-yl- 10W- phenoxazin-4-ol, 3v

Using an adaptation of the method described in Procedure 16, substituting eπc/o-3-(6-methoxy-3-pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2v for the TFA salt of 6- methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-ph enoxazine-3- carboxylic acid diethylamide, 6e, the title compound enc/o-10-(8-aza- bicyclo[3.2.1]oct-3-yl)-7-pyridin-3-yI-10H-phenoxazin~4-ol, 3v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 386.1.

£nc/o-7-Pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicy clo[3.2.1]oct- 3-yl)-10H-phenoxazin-4-ol, 4v

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-7-pyridin-3-yl- 10H-phenoxazin-4-ol, 3v for 4-amino-3-hydroxybenzoic acid methyl ester, and 2-pyridyl carboxaldehyde for 8-methyl-8-aza-bicyclo[3.2.1]octan-3-one, the title compound eπdo-7-pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[ 3.2.1]oct- 3-yl)-10H-phenoxazin-4-ol, 4v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 477.2.

E/7θfo-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-chloro-6-meth oxy-10H- phenoxazine, 5v

Using an adaptation of the method described in Procedure 25, substituting the TFA salt of e/?c/o-7-pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-4-ol, 4v for e/7cfo-3-(3-pyridin-3-yl- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j, the title compound endo-10-(8-aza~bicyclo[3.2.1]oct-3-yl)-3-chloro-6- methoxy-10H-phenoxazine, 5v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS /77/Z (MH ⁺ ) 357.1.

E/7c/o-10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-3-pyri din-3-yl- 10H-phenoxazine, 6v

Using an adaptation of the method described in Procedure 25, substituting enαfc>-3-(6-methoxy-3-pyridin-3-yl-phenoxazin-10-yl)-8-a za- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2v for eπdo-3-(3-pyridin- 3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxyl ic acid tert-butyl ester, 4j, the title compound enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy- 3-pyridin-3-yl-10H-phenoxazine, 6v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water

containing 0.1 % TFA). MS m/z (MH ⁺ ) 400.2.

Endo-6-Methoxy-3-pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8- aza- bicyclo[3.2.1]oct-3-yl)-10W-phenoxazine, 7v

Using an adaptation of the method described in Procedure 1 , substituting enαto-3-(6-methoxy-3-pyridin-3-yl-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 2v for 4-amino-3- hydroxybenzoic acid methyl ester, and 2-pyridyl carboxaldehyde for 8-methyl-8- aza-bicyclo[3.2.1]octan-3-one, the title compound endo-6-methoxy-3-pyridin-3- yl-10-(8-pyridin-2-ylmethyl -8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine, 7v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 491.2.

Endo-Λ O-t8-Aza-bicyclo[3.2.1]oct-3-yl)-y-pyridin-^yl-i OH- phenoxazin-4-ol, 8v

Using an adaptation of the methods described in Procedures 24 and 16, substituting eπc/o-3-(3-chloro-6-methoxy-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1v for endo-3-(3- chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1 ]-octane-8-carboxylic acid tert-butyl

ester, 3j, and 4-pyridylboronic acid for 3-pyridylboronic acid in Procedure 24, the title compound enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-7-pyridin-3-yl-10H- phenoxazin-4-ol, 8v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 386.2.

Endo-y-Pyridln-Φyl- 10-t8-pyNdin^-ylmethyl-3-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazin-4-ol, 9v

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of endo-10-(8-aza-bicyclo[3.2.1]oct-3-yl^-pyridin-3-yl- 10H-phenoxazin-4-ol, 8v for 4-amino-3-hydroxybenzoic acid methyl ester, and 2-pyridyl carboxaldehyde for 8-methyl-8-aza-bicyclo[3.2.1]octan-3-one, the title compound eπc/o-7-pyridin-3-yl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo [3.2.1]oct- 3-yl)-10H-phenoxazin-4-ol, 9v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 477.3.

Endo-10-t3-Phenethyl-8-aza-bicycIoIS^.IJoct-3-yO-T-pyridin-^ yl- 10H-phenoxazin-4-ol, 1Ov

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of eπc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-7-pyridin-3-yl- 10H-phenoxazin-4-ol, 8v for 4-amino-3-hydroxybenzoic acid methyl ester, and phenyl acetaldehyde for 8-methyl-8-aza-bicycio[3.2.1]octan-3-one, the title compound endo-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-7-pyridin -3-yl- 10H-phenoxazin-4-ol, 1Ov was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 490.3.

Endo- 10-t8-Aza-bicyclo[3.2.1]oct-3-yl)-e-methoxy-3-pyridin-^yl- 10H-phenoxazine, 11v

Using an adaptation of the methods described in Procedures 24 and 25, substituting endo-3-(3-chloro-6-methoxy-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane~8-carboxylic acid tert-butyl ester, 1v for endo-3-(3- chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1]-octane-8-carbox ylic acid tert-butyl ester, 3j, and 4-pyridylboronic acid for 3-pyridylboronic acid in Procedure 24, the title compound eπdo-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-3-pyridin- 4-yl-10H-phenoxazine, 11v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS /77/Z (MH ⁺ ) 400.2.

Ei7dO-6-Methoxy-3-pyridin-4-yl-10-(8-pyridin-2-ylmethyl-8 -aza- bicyclo[3.2.1]oct-3-yl)-10A/-phenoxazine, 12v

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of enc/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-3~ pyridin-3-yl-10H-phenoxazine, 11v for 4-amino-3-hydroxybenzoic acid methyl ester, and 2-pyridyl carboxaldehyde for 8-methyl-8-aza-bicyclo[3.2.1]octan-3- one, the title compound endo-6-methoxy-3-pyridin-3-yl-10-(8-pyridin-2-ylmethyl- 8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazine, 12v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 491.2.

Enc/o-6-Methoxy-10-(8-phenethyI-8-aza-bicyclo[3.2.1]oct-3 -yl)-3- pyridin-4-yl-1 OW-phenoxazine, 13v

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of θ/?c/o-10-(8-aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-3- pyridin-3-yl-10H-phenoxazine, 11v for 4-amino-3-hydroxybenzoic acid methyl

ester, and phenyl acetaldehyde for 8-methyl-8-aza-bicyclo[3.2.1]octan-3-one, the title compound endo-6-methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3- yl)-3-pyridin-3-yl-10H-phenoxazine, 13v was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 504.3.

EXAMPLE X

E/?do-3-[3-(2-Acetylaminophenyl)-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1x

Using an adaptation of the method described in Procedure 24, substituting N-[2-(4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-phenyl]- acetamide for 3-pyridyl boronic acid, the title compound enαto-3-[3-(2- acetylaminophenyl)-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]oct ane-8-carboxylic acid tert-butyl ester, 1x was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA).

Encfo-Λ/-{2-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10 W-phenoxazin-3-yl]- phenyl}-acetamide, 2x

Using an adaptation of Procedure 25, substituting the TFA salt of endo- 3-[3-(2-acetylaminophenyl)-phenoxazin-10-yl)-8-aza-bicyclo[3 .2.1]octane-8- carboxylic acid tert-butyl ester, 1x for enc/o-3-(3-pyridin-3-yl-phenoxazin-10-yl)- 8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 4j, the title compound eπc/o-Λ/-{2-[10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxa zin-3-yl)- phenyl}-acetamide, 2x was obtained as a TFA salt after purification via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 426.2.

Endo-yV^-ΪIO^δ-Phenethyl-δ-aza-bicyclo[3.2.1]oct-3-yl) -IOW- phenoxazin-3-yl)-phenyl}-acetamide, 3x

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of enc/o-Λ/-{2-[10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazin-3-yl)-phenyl}-acetamide, 2x for 4-amino-3-hydroxybenzoic acid methyl ester, and phenyl acetaldehyde for 8-methyl-8-aza-bicyclo[3.2.1]octan- 3-one, the title compound enc/o-Λ/-{2-[10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazin-3-yl)-phenyl}-acetamide, 3x was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 530.3.

£ndo-yV-{2-[10-(8-Thiophen-2-ylmethyl-8-aza-bicyclo[3.2. 1]oct-3-yl)-

10H-phenoxazin-3-yl)-phenyl}-acetamide, 4x

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of endo-Λ/-{2-[10-(8-aza-bicyclo[3.2.1]oct-3~yl)-10H- phenoxazin-3-yl)-phenyl}-acetamide, 2x for 4-amino-3-hydroxybenzoic acid methyl ester, and 2-thiophene carboxaldehyde for 8-methyl-8-aza- bicyclo[3.2.1]octan-3-one, the title compound θnc/o-Λ/-{2-[10-(8-thiophen-2- ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3-yl)- phenylJ-acetamide, 4x was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 522.2.

£ndo-/V-{2-[10-(8-Pyridin-2-yImethyl-8-aza-bicyclo[3.2.1 ]oct-3-yl)- 10W-phenoxazin-3-yl)-phenyl}-acetamide, 5x

Using an adaptation of the method described in Procedure 1 , substituting the TFA salt of e/?c/o-Λ/-{2-[10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenoxazin-3-yl)-phenyl}-acetamide, 2x for 4-amino-3-hydroxybenzoic acid methyl ester, and 2-pyridyl carboxaldehyde for 8-methyl-8-aza- bicyclo[3.2.1]octan-3-one, the title compound eπc/o-Λ/-{2-[10-(8-pyridin-2- ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenoxazin-3-yl)- phenyl}-acetamide, 5x was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 517.2.

EXAMPLE Y

2. TFA

£jfθ-Λ/-{2-[10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10W-phen oxazin-3-yl)- phenyl}-acetamide, 1y

Using an adaptation of the methods described in Procedures 24 and 25, and substituting exo-3-(3-chlorophenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane- 8-carboxylic acid tert-butyl ester, 11 for enc/o-3-(3-chlorophenoxazin-10-yl)-8- aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3j, and Λ/-[2-(4,4,5,5- tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-phenyl]-acetamide for 3-pyridyl boronic acid in Procedure 24, the title compound exo-Λ/-{2-[10-(8-aza-bicyclo[3.2.1]oct- 3-yl)-10H-phenoxazin-3-yl)-phenyl}-acetamide, 1y was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1% TFA). MS m/z (MH ⁺ ) 426.2.

EXAMPLE Z

Endo-3-[3-(2-Acetylaminophenyl)-6-methoxy-phenoxazin-10-y l)-8- aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1z

Using an adaptation of the method described in Procedure 24, substituting Λ/-[2-(4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-phenyl]- acetamide for 3-pyridyl boronic acid, the title compound endo-3-[3-(2- acetylaminophenyl)-6-methoxy-phenoxazin-10-yl)-8-aza-bicyclo [3.2.1]octane-8- carboxylic acid tert-butyl ester, 1z was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1% TFA).

Ent/o-yV^-I 10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-e-hydroxy- 10W- phenoxazin-3-yl]-phenyl}-acetamide, 2z

Using an adaptation of the method described in Procedure 16, substituting the TFA salt of enc/o-3-[3-(2-acetylaminophenyl)-6-methoxy- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1z for the TFA salt of e-methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10H-phenoxazine-3-carboxylic acid diethylamide, 6e, the title compound endo-

Λ/-{2-[10-(8-aza-bicyclo[3.2.1]oct-3-yl)-6-hydroxy-10H-phen oxazin-3-yl)-phenyl}- acetamide, 2z was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 442.2.

Endo-yV^-fiO^8-Aza-bicyclo[3.2.1]oct-3-yl^e-methoxy- 10W- phenoxazin-3-yl)-phenyl}-acetamide, 3z

Using an adaptation of the method described in Procedure 25, substituting the TFA salt of enofo-3-[3-(2-acetylaminophenyl)-6-methoxy- phenoxazin-10-yl)-8-aza-bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 1z for endo-3-(3-pyridin-3-yl-phenoxazin-10-yl)-8-aza-bicyclo[3.2.1 ]octane-8- carboxylic acid tert-butyl ester, 4j, the title compound endo-N-{2-[\ 0-(8-aza- bicyclo[3.2.1]oct-3-yl)-6-methoxy-10H-phenoxazin-3-yl)-pheny l}-acetamide, 3z ' was obtained as a TFA salt after purification via reverse phase chromatography (eiuent: CH ₃ CN in water containing 0.1 % TFA). MS m/z (MH ⁺ ) 456.2.

EXAMPLE AA

Procedure 28

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine-3-car bonitrile,

1aa

To a solution of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-bromo-10H- phenothiazine, 4i (50 mg, 0.13 mmol) in DMF (750 μl_) was added zinc cyanide (15 mg, 0.13 mmol) and tetrakistriphenylphosphine palladium (4 mg), the solution was purged with nitrogen, and the mixture was heated in the microwave for 5 min at 160 ⁰ C. The mixture was allowed to cool to rt, and purified via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA) to yield title compound 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine-3- carbonitrile, 1aa as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 334.1.

lO^8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-ylJ- 10H-phenothiazine-3- carbonitrile, 2aa

Using an adaptation of the method described in Procedure 22,

substituting the TFA salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine-3-carbonitrile, 1 aa for 3-bromo-10-piperidin-4-yl-1 OH- phenothiazine, 4i, the title compound 10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenothiazine-3-carbonitrile, 2aa was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1% TFA) and as a mixture of endo and exo isomers.

Procedure 29

lO^8-Phenethyl-8-aza-bicyclo[3.2.1]oct-3-ylJ-3-tiH-tetraz ol-3-yl)- 10H-phenothiazine, 3aa

To a solution of 10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine-3-carbonitrile, 2aa (10 mg, 29 μmol) in dimethoxyethane was added trimethylsilyl azide (15 μL, 115 μmol) and dibutyltin oxide (1.5 mg), and the mixture was heated in a microwave for 15 min at 150 ⁰ C. The mixture was allowed to cool to rt, and purified via reverse phase HPLC (eluent: CH ₃ CN in water containing 0.1% TFA) to yield title compound 10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-3^IH-tetrazol-8-yl)-10H-phenothiazin e, 3aa as a TFA salt and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 481.2.

lO-t8-Pyridin^-ylmethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-3-JIH- tetrazol- 5-yl)-10H-phenothiazine, 4aa

Using an adaptation of the methods described in Procedures 22 and 29, substituting the TFA salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H-

phenothiazine-3-carbonitrile, 1aa for 3-bromo-10-piperidin-4-yl-10H- phenothiazine, 4i and 2-pyridyl carboxaldehyde for 3-furyl carboxaldehyde in Procedure 22, the title compound 10-(8-pyridin-2-ylmethyl-8-aza~ bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-1 OH-phenothiazine, 4aa was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA) and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 468.2.

10-(8-Aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)-10 H- phenothiazine, 5aa Using an adaptation of the method described in Procedure

29, substituting the TFA salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine-3-carbonitrile, 1aa for 10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3- yl)-10H-phenothiazine-3-carbonitrile, 2aa, the title compound 10-(8-aza- bicyclo[3.2.1]oct-3-yl)-3-(1 H-tetrazol-5-yl)-1 OH-phenothiazine, 5aa was obtained as a TFA salt after purification via reverse phase chromatography

(eluent: CH ₃ CN in water containing 0.1% TFA) and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 377.2.

W,Λ/-Diethyl-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-y l)-10H- phenothiazine-3-carboxamidine, 6aa

Using an adaptation of the method described in Procedure 10, substituting the TFA salt of 10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)- I OH- phenothiazine-3-carbonitrile, 2aa for eπdo-3-(3-cyano-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3b, the title compound Λ/^-diethyl-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 0H-phenothiazine-3- carboxamidine, 6aa was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA) and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 511.3.

W,W-Diethyl-10-(8-pyridin-2-ylmethyl-8-aza-bicyclo[3.2.1] oct-3-yl)- 1 OH-phenothiazine-3-carboxamidine, 7aa

Using an adaptation of the methods described in Procedures 22 and 10, substituting the TFA salt of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine-3-carbonitrile, 1aa for enc/o-3-(3-cyano-phenoxazin-10-yl)-8-aza-

bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3b, and 2-pyridyl carboxaldehyde for 3-furyl carboxaldehyde, the title compound Λ/,Λ/-diethyl-10- (8-pyridin-2-ylmethyl-8-aza-bicycioP^.iloct-3-yl)-I OH-phenothiazine-3- carboxamidine, 7aa was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA) and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 498.3.

EXAMPLE BB

10-t8-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiaz ine-3- carbonϊtrile, 1bb

Using an adaptation of the method described in Procedure 28, substituting 3-bromo-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-1 OH- phenothiazine, 3i for enαto-3-(3-cyano-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3b, the title compound 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine -3-carbonitrile, 1bb was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1% TFA) and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 348.1.

lO^β-Methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-S^IH-tetrazol- 8-yl)- 10W- phenothiazine, 2bb

Using an adaptation of the method described in Procedure 29, substituting the TFA salt of 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-

phenothiazine-3-carbonitrile, 1bb for 10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3- yI)-10H-phenothiazine-3-carbonitrile, 2aa, the title compound 10-(8-methyl-8- aza-bicyclo[3.2.1]oct-3-yl)-3-(1H-tetrazol-5-yl)-10H-phenoth iazine, 2bb was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA) and as a mixture of endo and exo isomers.

MS /77/Z (MH ⁺ ) 391.1.

/V,N-Diethyl-10-(8-methyl-8-aza-bicycIo[3.2.1]oct-3-y I)- 10 H- phenothiazine-3-carboxamidine, 3bb

Using an adaptation of the method described in Procedure 10, substituting the TFA salt of 10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine-3-carbonitrile, 1 bb for endo-3-(3-cyano-phenoxazin-10-yl)-8-aza- bicyclo[3.2.1]octane-8-carboxylic acid tert-butyl ester, 3b, the title compound M/V-diethyl-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-p henothiazine-3- carboxamidine, 3bb was obtained as a TFA salt after purification via reverse phase chromatography (eluent: CH ₃ CN in water containing 0.1 % TFA) and as a mixture of endo and exo isomers. MS m/z (MH ⁺ ) 421.2.

EXAMPLE CC

Spiro compound, 1cc

Using an adaptation of the method described in Procedure 18, substituting for 2-amino-5-bromothiophenol for 2-aminothiophenol, the title compound 1cc was obtained.

[2-(3-Methoxy-2-nitro-phenylsuIfanyl)-phenyl]-(8-methyl-8-az a- bicyclo[3.2.1]oct-3-yl)-amine, 2cc

Using an adaptation of the method described in Procedure 19, substituting spiro compound, 1cc for spiro compound 1i, the title compound [2- (3-methoxy-2-nitro-phenylsulfanyl)-phenyl]-(8-methyl-8-aza-b icyclo[3.2.1]oct-3- yl)-amine, 2cc was obtained a mixture of endo and exo isomers.

3-Bromo-θ-methoxy- 10-t8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)- 10W- phenothiazine, 3cc

Using an adaptation of the method described in Procedure 20, substituting [2-(3~methoxy-2-nitro-phenylsulfanyl)-phenyl]-(3-rnethyl-8-a za- bicyclo[3.2.1]oct-3-yl)-amine, 2cc for [2-(4-bromo-2-nitrophenylsulfanyl)- phenylH8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-amine, 2i, the title compound 3- bromo- 6-methoxy-10^ 3-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine, 3cc was obtained a mixture of endo and exo isomers.

lO-f8-Aza-bicyclo[3.2.1]oct-3-yl)-3-bromo-β-methoxy- 10H- phenothiazine, 4cc

Using an adaptation of the method described in Procedure 21 , substituting 3-bromo-6-methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl) -1 OH- phenothiazine, 3cc for 3-bromo-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine, 3i, the title compound 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-bromo- 6-methoxy-10H-phenothiazine, 4cc was obtained a mixture of endo and exo isomers.

lO-t8-Aza-bicyclo[3.2.1]oct-3-yl)-T-bromo- 10H-phenothiazin^-ol,

5cc

Using an adaptation of the method described in Procedure 16, substituting 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-bromo-6-methoxy-1 OH- phenothiazine, 4cc for 6-methoxy-10-(8-phenethyl-8-aza-bicyclo[3.2.1]oct-3-yl)-

10H-phenoxazine-3-carboxylic acid diethylamide, 6e, a mixture of title compound 10-(8-aza-bicyclo-[3.2.1]oct-3-yl)-7-bromo-10H-phenothiazin- 4-ol, 5cc and starting material 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-bromo-8-methoxy- 10H-phenothiazine, 4cc was obtained a mixture of endo and exo isomers.

10-t8-Aza-bicyclo[3.2.1]oct-3-yl)- 6-methoxy-3-pyridin-3-yl-10//- phenothiazine, 6cc and 10-f 6-Aza-bicycIoIS^.Iloct-3-yl^-pyridin-3-yl-

10W-phenothiazin-4-ol, 7cc

Using an adaptation of the method described in Procedure 23, substituting a mixture of 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-3-bromo- 6-methoxy- 10H-phenothiazine, 4cc and 10-(8-aza-bicyclo-[3.2.1]oct-3-yl)-7-bromo-10H- phenothiazin-4-ol, 5cc for 3-bromo-10-(8-furan-3-ylmethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine, 5i, a mixture of title compounds 10- (8-aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy-3-pyridin-3-yl-10H- phenothiazine, 6cc and 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-y-pyridin-3-yl-10H-phenoth iazin^-ol, 7cc was obtained. The compounds were separated via reverse phase HPLC (eluent: CH3CN in water containing 0.1 % TFA) to yield 6cc [MS m/z (MH ⁺ ) 416.2] and 7cc [MS m/z (MH ⁺ ) 402.2], bothy as TFA salts and mixtures of endo and exo isomers.)

EXAMPLE DD

6-Methoxy-10^ 3-methyl-8-aza-bicycIolS^.iloct-3-yl)-10H- phenothiazine-3-carboxylic acid diethylamide, 1dd

Using an adaptation of the method described in Procedure 26, substituting a mixture of endo and exo isomers of 3-bromo-6-methoxy-10-(8- methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine, 3cc for 3-bromo-10- piperidin-4-yl-10H-phenothiazine, 4i, title compound 6-methoxy-10-(8-methyl-8- aza-bicyclo[3.2.1]oct-3-yl)-10H-phenothiazine-3-carboxylic acid diethylamide, 1dd was obtained as a mixture of endo and exo isomers.

lO-t8-Aza-bicyclo[3.2.1]oct-3-yl)-β-methoxy- 10H-phenothiazine-3- carboxylic acid diethylamide, 2dd

Using an adaptation of the method described in Procedure 21 , substituting 3-bromo-6-methoxy-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl) -1 OH- phenothiazine, 3cc for 3-bromo-10-(8-methyl-8-aza-bicyclo[3.2.1]oct-3-yl)-10H- phenothiazine, 3i, title compound 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-6-methoxy- 10H-phenothiazine-3-carboxylic acid diethylamide, 2dd was obtained as a TFA salt and as a mixture of endo and exo isomers.

lO^3-Aza-bicyclo[3.2.1]oct-3-yl)-β-hydroxy-10W-phenothiazin e-3- carboxylic acid diethylamide, 3dd

Using an adaptation of the method described in Procedure 16, substituting 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-8-methoxy-10H-phenothiazin e-3- carboxylic acid diethylamide, 2dd for 6-methoxy-10-(8-phenethyl-8-aza- bicyclo[3.2.1]oct-3-yl)-10/7-phenoxazine-3-carboxylic acid diethylamide, 6e, a mixture of title compound 10-(8-aza-bicyclo[3.2.1]oct-3-yl)-6-hydroxy-10H- phenothiazine-3-carboxylic acid diethylamide, 3dd was obtained. MS m/z (MH ⁺ ) 424.2.

Biological Examples

Rat Brain Delta Opioid Receptor Binding Assay

Procedure: Male, Wistar rats (150-250 g, VAF, Charles River, Kingston, NY) were killed by CO ₂ , and their brains were removed and placed immediately in ice cold Tris HCI buffer (50 mM, pH 7.4). The forebrains were separated from the remainder of the brain by a coronal transection, beginning dorsally at the colliculi and passing ventrally through the midbrain-pontine junction. After dissection, the forebrains were homogenized in Tris buffer in a Teflon ^® -giass homogenizer. The homogenate was diluted to a concentration of 1 g of forebrain tissue per 80 mL Tris and centrifuged at 39,000 x g for 10 min. The pellet was resuspended in the same volume of Tris buffer containing 5 mM MgCb with several brief pulses from a Polytron homogenizer. This particulate preparation was used for the delta opioid binding assays. Following incubation with the delta selective peptide ligand ~4 nM [ ³ H]DPDPE or 0.15 nM [ ³ H]naltrindole at 25°C for 2.5 h in a 96-well plate with total volume of 1 mL, the plate contents were filtered through Wallac filtermat B sheets on a Tomtec 96-well harvester. The filters were rinsed three times with 2 mL of 10 mM

HEPES (pH 7.4), and dried in a 650 W microwave oven for 1.75 min twice. To each sample area 2 X 50 μL of Betaplate Scint scintillation fluid (LKB) was added and the radioactivity quantified on a LKB (Wallac) 1205 BetaPlate liquid scintillation counter.

Analysis: The data from the scintillation counter were used to calculate either the % inhibition compared to control binding (when only a single concentration of test compound was evaluated) or a Kj value (when a range of concentrations was tested). Percent inhibition was calculated as: [(total dpm-test compound dpm)/(total dpm-nonspecific dpm)]*100. Kd and Ki values were calculated using GraphPad PRISM data analysis program.

Rat Brain Mu Opioid Receptor Binding Assay

Procedure: Male, Wistar rats (150-250 g, VAF, Charles River, Kingston, NY) were killed by CO ₂ , and their brains were removed and placed immediately in ice cold Tris HCI buffer (50 mM, pH 7.4). The forebrains were separated from the remainder of the brain by a coronal transection, beginning dorsally at the colliculi and passing ventrally through the midbrain-pontine junction. After dissection, the forebrains were homogenized in Tris buffer in a Teflon ^® -glass homogenizer. The homogenate was diluted to a concentration of 1 g of forebrain tissue per 80 mL Tris and centrifuged at 39,000 x g for 10 min. The pellet was resuspended in the same volume of Tris buffer containing 5 mM MgCb with several brief pulses from a Polytron homogenizer. This particulate preparation was used for the delta opioid binding assays. Following incubation with the mu selective peptide ligand, -0.8 nM [ ³ H]DAMGO, at 25°C for 2.5 h in a 96-well plate with total assay volume of 1 mL, the plate contents were filtered through Wallac filtermat B sheets on a Tomtec 96-well harvester. The filters were rinsed three times with 2 mL of 10 mM HEPES (pH 7.4), and dried in a 650 W microwave oven for 1.75 min twice. To each sample area 2 X 40 μL of Betaplate Scint scintillation fluid (LKB) was added and the radioactivity quantifed on a LKB (Wallac) 1205 BetaPlate liquid scintillation counter.

Analysis: The data from the scintillation counter were used to calculate either the % inhibition compared to control binding (when only a single concentration of test compound was evaluated) or a Kj value (when a range of concentrations tested). Percent inhibition was calculated as: [(total dpm-test compound dpm)/(total dpm-nonspecific dpm)]*100. Kd and Ki values were calculated using GraphPad PRISM data analysis program.

T ³⁵ SIGTPYS Binding Assay in NG108-15 Cell Membranes (delta opioid)

Methods: NG108-15 cell membranes can be purchased from Applied Cell Sciences (Rockville, MD). 8 mg/mL of membrane protein suspended in 10 mM TRI 3-HCI pH 7.2, 2 mM EDTA, 10% sucrose. Membranes can be maintained at 4-8 ⁰ C. A 1 ml_ volume of membranes can be added into 10 mL cold binding assay buffer. The assay buffer contained 50 mM Tris, pH 7.6, 5 mM MgCI ₂ , 100 mM NaCI, 1 mM DTT and 1 mM EGTA. The membrane suspension can be homogenized twice with a Polytron, and centrifuged at 3000 rpm for 10 min. The supernatant can be then centrifuged at 18,000 rpm for 20 min. Ten mL assay buffer can be added into the pellet containing tube. The pellet and buffer can be mixed with a Polytron.

Incubation procedure: The pellet membranes (75 μg/mL) can be preincubated with SPA (10 mg/mL) at 25°C for 45 min in the assay buffer. The SPA (5 mg/mL) coupled with membranes (37.5 μg/mL) can then be incubated with 0.1 nM [ ³⁵ S] GTPγS in the same Tris buffer containing 100 μM GDP in total volume of 200 μL. Increasing concentrations of receptor agonists can be used to stimulate [ ³⁵ S]- GTPDS binding. The basal binding can be tested in the absence of agonists and non-specific binding can be tested in the presence of 10 μM unlabeled GTPγS. The data can be analyzed on a Packard Top Count. DATA

% of Basal = (stimulated - non specific)*100/(basal - non specific). EC ₅₀ value values can be calculated using GraphPad Prism.

F ³⁵ SIGTPvS Binding Assays in CHO-hMOR Cell Membranes

Methods: CHO-hMOR cell membranes can be purchased from Receptor Biology, Inc. (Baltimore, MD). About 10 mg/mL of membrane protein can be suspended in 10 mM TRIS-HCI pH 7.2, 2 mM EDTA, 10% sucrose, and the suspension kept on ice. A 1 mL volume of membranes can be added to 15 ml_ cold binding assay buffer containing 50 mM HEPES, pH 7.6, 5 mM MgCI ₂ , 100 mM NaCI, 1 mM DTT and 1 mM EDTA. The membrane suspension can be homogenized with a Polytron and centrifuged at 3,000 rpm for 10 min. The supernatant can then be centrifuged at 18,000 rpm for 20 min. The pellet can be resuspended in 10 mL assay buffer with a Polytron. The membranes can be preincubated with wheat germ agglutinin coated SPA beads (Amersham) at 25 ⁰ C for 45 min in the assay buffer. The SPA bead (5 mg/mL) coupled membranes (10 μg/mL) can be then incubated with 0.5 nM [ ³⁵ S]GTPyS in the assay buffer. The basal binding can be that taking place in the absence of added test compound; this unmodulated binding can be considered as 100%, with agonist stimulated binding rising to levels significantly above this value. A range of concentrations of receptor agonist can be used to stimulate [ ³⁵ S]GTPyS binding. Both basal and non-specific binding can be tested in the absence of agonist; non-specific binding determination included 10 μM unlabeled GTPyS.

Compounds can be tested for function as antagonists by evaluating their potential to inhibit agonist-stimulated GTPyS binding. Radioactivity can be quantified on a Packard TopCount. The following parameters can be calculated:

EC _δ o values can be calculated using GraphPad Prism.

Biological Data

ind Number delta (Ki, nM) mu (Ki, r

4t 0.1

4aa 0.1

6b 0.1 168.45

6d 0.1 336

9t 0.10

8t 0.18

4v 0.3

2z 0.62

1Ot 1.14

7e 2.2

7t 2.43

7u 3.27

8v 5.2

3v 8.3

8j 18.8

8u 20.70

3t 25.1

3z 27.63

12v 36.3

6u 44.49

3c 48.1 525

7v 56.6

5t 56.7

5x 81.82

2t 86.6

6e 99.5

7j 103.6

7i 122.84 554.3

9v 136.3

4x 161.3

2u 196.5

6t 231.0

5k 246.7

6v 263.2

6i 282.35 1055.7

4e 320.9

5aa 321.1

11 i 366.9 170.985

4a 373 6761

Compound Number delta (Ki, nM) mu (Ki, nM)

3r 396.8 682.2

11v 399.5

5b 406 6849

5j 409.4

2x 414.6

4I 424.6

8i 443.5 889.4

4k 444.8

5u 477.80

3m 480.1

12i 491.45 1450

2c 492 9448

5I 539.2

4u 542.55

13i 551.6 3740

6a 575 819

2h 598 2889

17i 629 1533.5

15i 629.55 168.9

2m 634.2

9i 649.35 538.4

1 r 651.95 2758

2k 695.5

2a 697 5152

3I 729.1

2r 863.65 4340

4p 864.5

4m 868.9

4i 881 6613

1aa 930.9

18i 940.75 8073

10i 949.05 317.3

6I 1269.1

5m 1278.5

3u 1356.30

16i 1453 2654

3x 1585.0

1y 1601.8

14i 1603.5 598.75

3f 1752 1435

8b 1896 >10000

19 2041 4285

5a 2116 6091

5v 2260.0

3i 2532 3185

7d 2633 123

Compound Number delta (Ki, nM) mu (Ki, nM)

2f 2644.5 2752

4c 3106 89.0

6j 3261.5

3k 3388.0

3p 5123.0

19i 5191 254

7b 5475 288

7\ 6211.5

9j 11865.0

10j 13321.5

6k 14223.5

10v >10000

13v >10000