Title:
TRANSGENIC ANIMALS
Document Type and Number:
WIPO Patent Application WO2000030437
Kind Code:
A9
Abstract:
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous 5nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
Inventors:
BREMEL ROBERT D (US)
BLECK GREGORY T (US)
BLECK GREGORY T (US)
Application Number:
PCT/US1999/026848
Publication Date:
May 17, 2001
Filing Date:
November 17, 1999
Export Citation:
Assignee:
WISCONSIN ALUMNI RES FOUND (US)
BREMEL ROBERT D (US)
BLECK GREGORY T (US)
BREMEL ROBERT D (US)
BLECK GREGORY T (US)
International Classes:
A01K67/027; C07K14/02; C12N15/85; (IPC1-7): A01K67/027; C12N15/00; C12P21/00
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