Title:
UNIVERSAL TAGS WITH NON-NATURAL NUCLEOBASES
Document Type and Number:
WIPO Patent Application WO/2010/135384
Kind Code:
A3
Abstract:
The present invention relates to amplification primers with a universal tag and sequencing primers comprising at least one non-natural nucleobase capable of hybridizing to a complementary non-natural nucleobase. The present invention further relates to amplification methods of nucleic acid amplification and sequencing using an amplification primer with a universal tag and sequencing primers, as well as kits and solid supports comprising such primers and tags.
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Inventors:
BATTERSBY THOMAS (US)
GUETTOUCHE TOUMY (DE)
HNATYSZYN JAMES (US)
GUETTOUCHE TOUMY (DE)
HNATYSZYN JAMES (US)
Application Number:
PCT/US2010/035339
Publication Date:
January 20, 2011
Filing Date:
May 19, 2010
Export Citation:
Assignee:
SIEMENS HEALTHCARE DIAGNOSTICS (US)
BATTERSBY THOMAS (US)
GUETTOUCHE TOUMY (DE)
HNATYSZYN JAMES (US)
BATTERSBY THOMAS (US)
GUETTOUCHE TOUMY (DE)
HNATYSZYN JAMES (US)
International Classes:
C07H21/04
Foreign References:
| US20080299568A1 | 2008-12-04 | |||
| US20070264694A1 | 2007-11-15 | |||
| US20070020667A1 | 2007-01-25 | |||
| US20070015200A1 | 2007-01-18 | |||
| US20040259118A1 | 2004-12-23 |
Other References:
JOHNSON ET AL: "Multiplexed genetic analysis using an expanded genetic alphabet", CLIN. CHEM., vol. 50, no. 11, 2004, pages 2019 - 2027, XP055068742
UGOZZOLI ET AL: "Multiplex assays with fluorescent microbead readout: a powerful tool for mutation detection", CLIN. CHEM., vol. 50, no. 11, 2004, pages 1963 - 1965, XP055068743
AHLE ET AL: "Sequence determination of nucleic acids containing 5-methylisocytosine and isoguanine: identification and insight into polymerase replication of the non-natural nucleobases", NUCLEIC ACIDS RES., vol. 33, no. 10, 2005, pages 3176 - 3184, XP055068744
JOHNSON ET AL: "A third base pair for the polymerase chain reaction: inserting isoC and isoG", NUCLEIC ACIDS RES., vol. 32, no. 6, 2004, pages 1937 - 1941, XP002486990
UGOZZOLI ET AL: "Multiplex assays with fluorescent microbead readout: a powerful tool for mutation detection", CLIN. CHEM., vol. 50, no. 11, 2004, pages 1963 - 1965, XP055068743
AHLE ET AL: "Sequence determination of nucleic acids containing 5-methylisocytosine and isoguanine: identification and insight into polymerase replication of the non-natural nucleobases", NUCLEIC ACIDS RES., vol. 33, no. 10, 2005, pages 3176 - 3184, XP055068744
JOHNSON ET AL: "A third base pair for the polymerase chain reaction: inserting isoC and isoG", NUCLEIC ACIDS RES., vol. 32, no. 6, 2004, pages 1937 - 1941, XP002486990
Attorney, Agent or Firm:
SIEMENS CORPORATION (170 Wood Avenue SouthIselin, NJ, US)
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