Title:
【発明の名称】高められた光学純度を有する化合物の製造方法
Document Type and Number:
Japanese Patent JP2002533086
Kind Code:
A
Abstract:
Process for the preparation of a compound with enhanced optical purity wherein a mixture of the enantiomers of a chiral compound of formula 1 wherein: R1 represents an alkyl or an aryl group R2 represents H, an alkyl or an aryl group Y represents an alkyl group, an aryl group, (CH2)nCOOH, (CH2)n-COOR, (CH2)n-CONRR', CH2OH, or C IDENTICAL N wherein R and R' independently represent H, an alkyl or aryl group, and n represents 0 or 1, is brought into contact with an enzyme having peptide deformylase activity with a bivalent metal ion as a cofactor wherein the metal is chosen from the groups 5-11 of the periodic system, or for the preparation of a formylated compound with enhanced optical purity from a mixture of the enantiomers of the corresponding not formylated chiral compound in the presence of a formylation agent. Preferably the peptide deformylase is chosen from the class EC 3.5.2.27 or EC 3.5.1.31, and contains the sequences of (I) HEXXH, (ii) EGCLS and (iii) GXGXAAXQ. The bivalent metal may be chosen from the group of Fe, Ni, Mn and Co, preferably Ni or Fe.
Inventors:
Quaid Freag, Peter, Jean, Leonardo, Mario
Songke, Theodorus
Wagner, Adolf, Fritz, Volker
Songke, Theodorus
Wagner, Adolf, Fritz, Volker
Application Number:
JP2000589711A
Publication Date:
October 08, 2002
Filing Date:
December 17, 1999
Export Citation:
Assignee:
DSM N.V.
International Classes:
C12N9/78; C12N15/55; C12P13/04; C12P41/00; (IPC1-7): C12P41/00; C12N9/78
Attorney, Agent or Firm:
Mitsuo Matsui