Title:
核酸配列の複数コピーを作製するための方法および組成物、ならびにそれらを検出する方法
Document Type and Number:
Japanese Patent JP2005503755
Kind Code:
A
Abstract:
The present invention provides novel isothermal methods of generating multiple copies of, detecting and/or quantifying nucleic acid sequences of interest based on limited primer extension or attachment of oligonucleotide pairs using composite RNA/DNA primers. Methods for generating multiple copies of and/or detecting and/or quantifying nucleic acid sequences, wherein products of primer extension or attachment of oligonucleotide pairs comprising a cleavable portion are generated, and wherein cleavage of the products results in dissociation of cleaved products from target polynucleotides, are provided. The invention further provides compositions, kits and systems for practicing these methods.
Inventors:
Khan, Nuris
Application Number:
JP2002550116A
Publication Date:
February 10, 2005
Filing Date:
December 13, 2001
Export Citation:
Assignee:
Nugen Technologies, Inc.
International Classes:
G01N33/53; C12M1/00; C12N15/09; C12Q1/68; C12Q1/6823; C12Q1/6827; C12Q1/6853; C12Q1/6869; G01N33/566; (IPC1-7): C12Q1/68; C12N15/09; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita
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