Title:
病原体の多重定量検出方法
Document Type and Number:
Japanese Patent JP2009514551
Kind Code:
A
Abstract:
The invention allows for the quantitative detection of a plurality of pathogens in a single sample. The method includes the amplification of a sample with a plurality of pathogen-specific primer pairs to generate amplicons of distinct sizes from each of the pathogen specific primer pairs. The method further includes the use of a plurality of competitor polynucleotide targets that correspond to each of the pathogen-specific primer pairs. The competitor polynucleotides are added to the reaction mixture at a known concentration to allow for the quantitation of the amount of pathogen in the sample. The method can be used for monitoring pathogen infection in an individual, preferably an immunocompromised individual.
Inventors:
Threfneb Vladimir eye.
Shiozaki Kazumi
Heart kyle
Garcia Elizabeth
Shiozaki Kazumi
Heart kyle
Garcia Elizabeth
Application Number:
JP2008540163A
Publication Date:
April 09, 2009
Filing Date:
November 09, 2006
Export Citation:
Assignee:
Primera Biosystems Incorporated
International Classes:
C12Q1/68; G01N21/78
Attorney, Agent or Firm:
Hatsushi Shimizu
Toshi Gobe
Koichi Niimi
Tomohiko Kobayashi
Shinichi Watanabe
Ryuichi Inoue
Masato Ozeki
Toshi Gobe
Koichi Niimi
Tomohiko Kobayashi
Shinichi Watanabe
Ryuichi Inoue
Masato Ozeki