Title:
MODIFIED MULLERIAN INHIBITING SUBSTANCE (MIS) PROTEINS AND USES THEREOF FOR TREATMENT OF DISEASES
Document Type and Number:
Japanese Patent JP2020074789
Kind Code:
A
Abstract:
To provide modified recombinant human Mullerian Inhibiting Substance (MIS) proteins which have improved cleavage, increased bioactivity, and increased potency, as compared to wild-type human MIS proteins.SOLUTION: Provided is a recombinant MIS protein comprising a combination of a modification of a non-MIS leader sequence or a functional fragment thereof in place of the MIS leader sequence in a particular amino acid sequence and a modification of at least one amino acid in a particular amino acid sequence, to increase cleavage as compared to in the absence of a modification, the recombinant MIS protein having increased cleavage and increased yield of production in vitro as compared to wild-type MIS protein.SELECTED DRAWING: Figure 1B
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Inventors:
PATRICIA K DONAHOE
DEMETRIOS VAVVAS
DAVID PEPIN
HOANG MIEN VAN
DEMETRIOS VAVVAS
DAVID PEPIN
HOANG MIEN VAN
Application Number:
JP2020010494A
Publication Date:
May 21, 2020
Filing Date:
January 27, 2020
Export Citation:
Assignee:
MASSACHUSETTS GEN HOSPITAL
MASSACHUSETTS EYE & EAR INFIRMARY
MASSACHUSETTS EYE & EAR INFIRMARY
International Classes:
C12N15/12; A61K9/08; A61K9/12; A61K31/337; A61K31/4188; A61K31/436; A61K31/706; A61K33/24; A61K35/76; A61K38/02; A61K45/00; A61K48/00; A61P3/06; A61P3/10; A61P15/08; A61P17/00; A61P17/14; A61P35/00; A61P43/00; C07K14/495; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/62; C12N15/70; C12N15/861; C12N15/863; C12N15/867
Domestic Patent References:
| JP6698015B2 | 2020-05-27 | |||
| JP2003509377A | 2003-03-11 | |||
| JP2007506420A | 2007-03-22 | |||
| JPS62190084A | 1987-08-20 | |||
| JPH02117384A | 1990-05-01 |
Other References:
PROTEIN EXPRESSION AND PURIFICATION (2010) VOL.70, PP.32-38, JPN6018004666, ISSN: 0004637515
PROTEIN EXPRESSION AND PURIFICATION (2004) VOL.37, PP.265-272, JPN6018004670, ISSN: 0004637516
PNAS (2006) VOL.103, NO.46, PP.17426-17431, JPN6018004669, ISSN: 0004637517
CLINICAL CANCER RESEARCH (1995) VOL.1, PP.343-349, JPN6018004671, ISSN: 0004637518
PROTEIN EXPRESSION AND PURIFICATION (2004) VOL.37, PP.265-272, JPN6018004670, ISSN: 0004637516
PNAS (2006) VOL.103, NO.46, PP.17426-17431, JPN6018004669, ISSN: 0004637517
CLINICAL CANCER RESEARCH (1995) VOL.1, PP.343-349, JPN6018004671, ISSN: 0004637518
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto