Title:
核酸増幅方法を改良するためのRNAポリメラーゼの使用
Document Type and Number:
Japanese Patent JP3881009
Kind Code:
B2
Abstract:
This invention relates to the use of a eukaryotic or prokaryotic DNA-directed RNA polymerase of a class that synthesizes cellular RNA, in a process for the amplification of a specific nucleic acid sequence or of its complement. It also relates to a new process for amplifying a specific nucleic acid sequence. The process includes one or more reactions which may take place in a single reaction vessel. In one instance, in a first reaction, the process includes providing a first RNA polymerase which uses a DNA first template to synthesize an RNA first template, and, in a second reaction, providing the RNA first template and a number of other reagents such that the reagents use the RNA first template to synthesize a DNA second template and an RNA second template. Thereafter a cycle ensues in which the reagents use the RNA second template to synthesize a DNA third template and multiple copies of the RNA second template. The RNA second template is the specific nucleic acid sequence or its complement. This invention includes a kit containing the reagents of this invention.
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Inventors:
Souknanan, Roy
Application Number:
JP50454596A
Publication Date:
February 14, 2007
Filing Date:
July 13, 1995
Export Citation:
Assignee:
Biomerio B.B.
International Classes:
C12Q1/68; C12Q1/6865
Domestic Patent References:
| JP2005864A | ||||
| JP4500759A |
Attorney, Agent or Firm:
Yoshio Kawaguchi
Mitsuru Inoue
Akio Ichiiri
Katsuma Osaki
Takamasa Soma
Mitsuru Inoue
Akio Ichiiri
Katsuma Osaki
Takamasa Soma