Title:
CRM蛋白質およびジフテリアトキシンを産生させるための新規なプラスミド
Document Type and Number:
Japanese Patent JP4144813
Kind Code:
B2
Abstract:
The invention pertains to a novel method and plasmid system for producing abundant quantities of CRM197 protein, diphtheria toxin or other CRM proteins related to diphtheria toxin, as well as to microorganisms transformed with the novel plasmid. A particularly preferred DNA plasmid, designated pPX 3511, that combines the gene for CRM197 from the nontoxigenic betaphage and the plasmid pNG2-22 is described. The novel plasmid system is capable of transforming strains of Corynebacterium diphtheriae into strains which are capable of expressing high levels of the CRM197 protein without the use of multiple lysogens. The invention provides in elegant means for increasing protein production without having to manipulate the expression vector, such as by increasing the promoter strength, or removing the promoter from iron regulation.
Inventors:
Benjamin Jei Metcalf
Application Number:
JP5442894A
Publication Date:
September 03, 2008
Filing Date:
March 01, 1994
Export Citation:
Assignee:
Wyeth Holdings Corporation
International Classes:
C12N1/21; C07K14/34; C12P21/00; C12N15/09; C12N15/77; C12R1/16
Domestic Patent References:
| JP60227681A |
Other References:
Proc.Natl.Acad.Sci.USA(1983)Vol.80,No.22,p.6853-6857
The Journal of Biological Chemistry(1973)Vol.248,No.11,p.3838-3844
FEMS Microbiology Letters(1990)Vol.66,p.119-123
The Journal of Biological Chemistry(1973)Vol.248,No.11,p.3838-3844
FEMS Microbiology Letters(1990)Vol.66,p.119-123
Attorney, Agent or Firm:
Patent business corporation Odashima patent office
Heikichi Odashima
Heikichi Odashima