Title:
プロテアーゼ操作を介した代謝経路におけるフラックスの制御のための方法
Document Type and Number:
Japanese Patent JP6407228
Kind Code:
B2
Abstract:
The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.
Inventors:
Swarts, James, Earl.
Application Number:
JP2016200349A
Publication Date:
October 17, 2018
Filing Date:
October 11, 2016
Export Citation:
Assignee:
GREENLIGHT BIOSCIENCES,INC.
International Classes:
C12N1/21; C12P1/04; C12P7/06
Domestic Patent References:
| JP8502176A | ||||
| JP10511845A |
Attorney, Agent or Firm:
Kiyoji Kuzuwa