Title:
キャリブレータを考慮したメタゲノミクス分析による標的生物種の検出および定量方法
Document Type and Number:
Japanese Patent JP7483855
Kind Code:
B2
Abstract:
A method for detecting a biological species of interest (SOI) potentially present in an analysis sample, the biological species of interest having a known or partially known genome, the analysis sample comprising a mixture of various biological species, the methodcomprising (a) extracting nucleic acids from the analysis sample; (b) sequencing nucleotide sequences extracted, (c) on the basis of the sequencing result, (i) assigning the sequences resulting from the sequencing, based on a reference database of sequences; (i) assigning sequences; (ii) determining a quantity (RSOI, RNSOI) of sequences assigned to the biological species of interest; and, prior to the sequencing, adding a calibrator, the calibrator being a biological species added in a known concentration, to the analysis sample, the calibrator having a known genome, and on the basis of the sequencing result, determining a quantity (RCAL) of sequences assigned to the calibrator; (d) on the basis of the quantities of sequences estimated in the determining of (RSOI, RNSOI) and (RCAL), estimating a concentration (CSOI) of the biological species of interest (SOI) in the sample.
Inventors:
Lazarevich Vladimir
Hauser Sebastian
Tournoud Moud
Hauser Sebastian
Tournoud Moud
Application Number:
JP2022504012A
Publication Date:
May 15, 2024
Filing Date:
July 22, 2020
Export Citation:
Assignee:
BIOMERIEUX
International Classes:
C12Q1/04; C12Q1/6869; G16B30/10
Other References:
BioTechniques,2018年,Vol.65, pp.315-321
Microbiome,2016年,Vol.4, 28 (13 pages)
Microbiome,2016年,Vol.4, 28 (13 pages)
Attorney, Agent or Firm:
Ken Ieiri