To provide an anisotropic conductive sheet in which conductive parts are exchangeable and each conductive part is readily handled and to provide a manufacturing method of the anisotropic conductive sheet.

An anisotropic conductive sheet 10A includes, while including a plurality of pin insertion holes 21, frame bodies 20A and 20B having insulation quality of preventing conduction of electricity, and a conductive part 312 with conductivity of conducting the electricity. The conductive part 312 is positioned at the pin insertion hole 21 and protrudes from surfaces of the frame bodies 20A and 20B and includes conductive removable members 30 which are removably attached to the frame bodies 20A and 20B.