Title:
発現自動制御コロニーアッセイ法
Document Type and Number:
Japanese Patent JP7364242
Kind Code:
B2
Abstract:
The present invention provides a colony assay method in which it is possible to automatically induce expression following colony formation and automatically stop expression after expression has occurred, the method being an "automatic expression control colony assay method" whereby screening for the expression of a functional protein that recognizes a target such as an antibody can be easily and reliably performed. The method is characterized in that neither glucose nor an expression inducer is added to a solid microorganism nutrient medium, and sugar or an expression inducer such as IPTG is added in an optimal range, along with glucose, to a microorganism inoculation liquid. In addition, the detection process can be made faster and easier by using an expression library in which alkaline phosphatase (AP) has been fused to the N-terminal of the protein.
Inventors:
Mieko Kamada
Application Number:
JP2020551102A
Publication Date:
October 18, 2023
Filing Date:
October 04, 2019
Export Citation:
Assignee:
Bio Peak Co., Ltd.
International Classes:
C12Q1/02; G01N33/15; G01N33/50; G01N33/53
Domestic Patent References:
| JP2017073995A |
Other References:
J. Biotechnol.,2017年,vol.255,p.1-8
Attorney, Agent or Firm:
Takuro Saeki
Tadashi Nakamura Exhibition
Yuko Saeki
Tadashi Nakamura Exhibition
Yuko Saeki