Title:
CLEAVAGE OF CHIMERA PROTEIN WITH PROCESSING ENZYME
Document Type and Number:
Japanese Patent JP3925982
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a new chimera protein containing a part rich in histidine residues at the N-terminal and capable of being easily cleave with a processing enzyme to efficiently give an objective protein as a substrate for the production of the physiologically active peptide (precursor).
SOLUTION: A new chimera protein of the formula: A-L-B {A is a protecting protein; B is an objective protein; L is a linker peptide; the C-terminal part has the sequence: X1-X2-(Pro, Lys or Arg)-Arg (X1, X2 are each an arbitrary amino acid); the N-terminal part has a His-enriched part such as the sequence: [(His)4-Pro-Gly]n [(n) is 1-6]}. As a substrate for producing the physiologically active peptide or its precursor, the chimera protein can easily be cleaved with a processing enzyme to efficiently give the objective protein. The chimeral protein is obtained by expressing a vector containing a gene bound to a DNA coding the protecting peptide, the linker peptide and the objective peptide, respectively, in a host cell.
Inventors:
Suzuki Yuji
Koji Sonoda
Masashi Toyofumi
Koji Sonoda
Masashi Toyofumi
Application Number:
JP6398997A
Publication Date:
June 06, 2007
Filing Date:
March 04, 1997
Export Citation:
Assignee:
Daiichi Asubi Pharma Co., Ltd.
International Classes:
C12N15/09; C07H21/04; C07K1/20; C07K1/30; C07K14/195; C07K14/41; C07K14/575; C07K14/585; C07K14/635; C07K19/00; C12N1/21; C12N9/38; C12N9/50; C12N15/16; C12N15/56; C12N15/62; C12P21/02; C12R1/19; (IPC1-7): C07K19/00; C07H21/04; C07K1/30; C07K14/635; C12N15/09; C12P21/02; //C12N9/50; (C12P21/02; C12R1:19)
Domestic Patent References:
| JP4148694A | ||||
| JP5505940A | ||||
| JP5304976A | ||||
| JP4504846A |
Other References:
Yuji Suzuki et al.,High-Level Production of Recombinant Human Parathyroid Hormone 1-34,Applied and Environmental Microbiology,1998年,Vol.64, No.2,pp.526-529
Charles Brenner et al.,Structual and enzymatic characterization of a purified prohormone-processing enzyme: Secreted, souble Kex2 protease,Proc.Natl.Acad.Sci.USA ,米国,1992年 2月,Vol.89,922-926
Charles Brenner et al.,Structual and enzymatic characterization of a purified prohormone-processing enzyme: Secreted, souble Kex2 protease,Proc.Natl.Acad.Sci.USA ,米国,1992年 2月,Vol.89,922-926
Attorney, Agent or Firm:
Masaya Nishiyama
Fukumoto product
Takashi Ishida
Fukumoto product
Takashi Ishida