Title:
PROBE FOR DETECTION OF NUCLEIC ACID AND DETECTION OF NUCLEIC ACID UTILIZING THE SAME PROBE
Document Type and Number:
Japanese Patent JP3925981
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject probe for detection of a nucleic acid, capable of forming an intermolecular bond, having high detection sensitivity and simplified in operation by providing a sequence complementary to a prescribed sequence which a target nucleic acid molecule has and providing terminal sequences at the both ends.
SOLUTION: A sequence 11 which is complementary to a prescribed sequence which a target nucleic acid molecule to be detected has is provided so as to form a complex with the target nucleic acid through the part and further, a self-complementary sequence 13 inserting spacer sequences 12 at both ends of the complementary sequence 11 is provided and an intramolecular bond is formed through the self-complementary sequence 13 so as to form a cyclic structure and each sequence is arranged so as to improve stability when a complex is formed between a probe and the target nucleic acid to provide the objective probe 10 for detection of nucleic acid, used for a detection method of nucleic acid which is increased in detection sensitivity and simplified in experimental operation and further reduced in cost, compared with a conventional detection method of nucleic acid.
Inventors:
Koji Shigemori
Jun Fujiwara
Jun Fujiwara
Application Number:
JP6018197A
Publication Date:
June 06, 2007
Filing Date:
February 28, 1997
Export Citation:
Assignee:
Aisin Cosmos Research Institute Co., Ltd.
International Classes:
C12Q1/68; G01N33/53; C07H21/04; C12N15/09; G01N33/566; (IPC1-7): C12Q1/68; //C07H21/04; C12N15/09; G01N33/53; G01N33/566
Foreign References:
| US5514546 | ||||
| US5563034 | ||||
| US5215899 | ||||
| EP0466367A1 | ||||
| EP0427074A1 | ||||
| WO1996017079A1 | ||||
| WO1995018236A1 | ||||
| US5426180 | ||||
| US5176996 | ||||
| WO1992010590A1 | ||||
| WO1992009705A1 | ||||
| US4888274 |
Other References:
Proc Natl Acad Sci U S A. 1988, Vol.85, No.17, p.6242-6246
Nucleic Acids Res. 1993, Vol.21, No.11, p.2729-2735
Biochem Biophys Res Commun. 1988, Vol.157 No.3, p.986-991
J Biol Chem. 1985, Vol.260, No.1, p.170-173
Proc Natl Acad Sci U S A. 1984, Vol.81, No.22, p.7026-7030
Proc Natl Acad Sci U S A. 1985, Vol.82 ,No.2, p.297-301
J Biol Chem. 1992, Vol.267, No.6, p.4215-4222
Nucleic Acids Res. 1996, Vol.24, No.17, p.3478-3480
山本たけ万 他,高効率DNAマッピング技術の研究,バイオテクノロジーシンポジウム予稿集,1996年11月15日,Vol.14,p.185-190
Nucleic Acids Res. 1993, Vol.21, No.11, p.2729-2735
Biochem Biophys Res Commun. 1988, Vol.157 No.3, p.986-991
J Biol Chem. 1985, Vol.260, No.1, p.170-173
Proc Natl Acad Sci U S A. 1984, Vol.81, No.22, p.7026-7030
Proc Natl Acad Sci U S A. 1985, Vol.82 ,No.2, p.297-301
J Biol Chem. 1992, Vol.267, No.6, p.4215-4222
Nucleic Acids Res. 1996, Vol.24, No.17, p.3478-3480
山本たけ万 他,高効率DNAマッピング技術の研究,バイオテクノロジーシンポジウム予稿集,1996年11月15日,Vol.14,p.185-190
Attorney, Agent or Firm:
Takehiko Suzue
Sadao Muramatsu
Ryo Hashimoto
Satoshi Kono
Makoto Nakamura
Sadao Muramatsu
Ryo Hashimoto
Satoshi Kono
Makoto Nakamura
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