Title:
試験試料中の白血球細胞、エステラーゼ又はプロテアーゼを測定するための組成物、方法及び試験具
Document Type and Number:
Japanese Patent JP4033921
Kind Code:
B2
Abstract:
Lactate esters of formula BOOC-CHMe-OA (I) are new. A = an OH-protecting gp.; B = the residue of a cpd. B-OH and provides a detectable response when the ester is hydrolysed to generate B-OH, the response being chromogenic, fluorescent, a reflectance change, a pH change, chemiluminescent, colorimetric or spectrophotometric. Also claimed is a compsn. for determining the presence or concn. of leukocytes, esterases or proteases in a test sample, comprising a cpd. (I), a buffer, opt. an accelerator for increasing the hydrolysis rate of (I), and opt. a diazonium salt. Also claimed is a method for assaying a liq. sample for the presence or concn. of leukocytes, esterases or proteases, comprising contacting the sample with a compsn. as above, examining the compsn. for a detectable response, and correlating the response to the presence or concn. of leukocytes, esterases or proteases in the sample.
Inventors:
Gary Michael Johnson
Robert Jay Shaper
Robert Jay Shaper
Application Number:
JP21164895A
Publication Date:
January 16, 2008
Filing Date:
August 21, 1995
Export Citation:
Assignee:
Bayer Corporation
International Classes:
C07C69/12; G01N33/50; C07C69/22; C07C69/67; C07C69/68; C07C69/76; C07C69/92; C07C309/64; C07C309/72; C07D207/36; C07D307/58; C07D333/32; C12Q1/00; C12Q1/04; C12Q1/37; C12Q1/44; G01N33/52
Domestic Patent References:
| JP60260558A | ||||
| JP60256398A | ||||
| JP60181048A | ||||
| JP5529590A |
Attorney, Agent or Firm:
Hajime Tsukuni
Fusayuki Saito
Hiroshi Kazama
Fusayuki Saito
Hiroshi Kazama