Title:
DETERMINATION OF INTERFERON ACTIVITY
Document Type and Number:
Japanese Patent JP3467515
Kind Code:
B2
Abstract:
PURPOSE: To easily determine the activity of interferon useful as an antiviral agent or an antitumor agent in improved sensitivity by reacting a specimen containing an interferon with a cell and determining the induced protein.
CONSTITUTION: A specimen containing interferon (e.g. interferon-3a or interferon-β) such as serum, blood plasma, buffer solution, medium or supernatant of cell culture medium is added to e.g. a buffer solution containing suspended cells such as leukocyte, monocyte, lymphocyte, fibroblast or epithelial cell or established cells such as THP-1 cell, U-937 cell, H9 cell, FL cell or WISH cell, incubating the mixture under a cell culture condition for 3-48hr and determining the induced protein composed of an enzyme such as 2'→5' oligoadenylic acid synthetase or phosphodiesterase to easily determine the biological activity of interferon in high sensitivity compared with conventional method.
Inventors:
Katsuko Uno
Kotaro Kishida
Takayuki Sato
Yoshihiro Takada
Keiji Fujioka
Kotaro Kishida
Takayuki Sato
Yoshihiro Takada
Keiji Fujioka
Application Number:
JP29372494A
Publication Date:
November 17, 2003
Filing Date:
November 01, 1994
Export Citation:
Assignee:
Louis Pasteur Medical Research Center
International Classes:
C12N5/08; C12Q1/02; C12Q1/25; C12Q1/42; G01N33/68; (IPC1-7): C12Q1/02; C12Q1/25; G01N33/68
Domestic Patent References:
| JP62115291A | ||||
| JP55144898A |
Attorney, Agent or Firm:
Yoshinori Hosoda
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