To provide a method in which a limit to a measuring container, an irradiation direction or the lower limit of the amount of a sample solution when multiple-chain nucleic acid comprising a specific base sequence is detected or quantitatively determined by using a fluorescent pigment.
This detecting method for multiple-chain nucleic acid comprises (1) a process wherein a fluorescent pigment which emits fluorescence under the existence of the multiple-chain nucleic acid or which has a fluorescent characteristic to increase the fluorescence and which can maintain the fluorescent characteristic even in a dry state is added to a sample solution as an object used to detect or quantitatively determine the multiple-chain nucleic acid. The detecting method comprises (2) a process wherein a known amount of the sample solution to which the fluorescent pigment is added is placed on a clean substrate for observation and the sample solution is dried. The detecting method comprises (3) a process wherein the fluorescence from the dried sample solution is measured and the multiple-chain nucleic acid in the sample solution is detected or quantitatively determined.
SUZUKI TOMOHIRO
YAMAMOTO NOBUKO
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