Title:
多量体タンパク質の純度の決定
Document Type and Number:
Japanese Patent JP7137663
Kind Code:
B2
Abstract:
Improved capillary zone electrophoresis (CZE), affinity capillary electrophoresis (ACE), and partially filled-ACE (PF-ACE) systems and methods for the detection and quantification of specific molecular entities in a mixture thereof are provided. During manufacturing, heterodimeric bispecific antibodies are often produced along with homodimer species, which can confound quantification of the bispecific antibody. Disclosed are capillary electrophoretic systems and methods of detecting a specific homodimer in the mixture of bispecific heterodimer and homodimers. A ligand capable of binding one of the subunits of the bispecific antibody is contacted with the mixture to form a complex having a reduced electrophoretic mobility, thereby enabling detection of the unbound homodimer.
Inventors:
mussami katil
Luo Jiang-kae
Luo Jiang-kae
Application Number:
JP2021092988A
Publication Date:
September 14, 2022
Filing Date:
June 02, 2021
Export Citation:
Assignee:
REGENERON PHARMACEUTICALS, INC.
International Classes:
C12M1/34; C07K1/26; G01N33/531; G01N33/536
Domestic Patent References:
| JP2015510764A | ||||
| JP2009139255A | ||||
| JP2002517407A |
Other References:
Introducing CaptureSelect Affinity Ligands for Antibody Detection and Characterization,[online],2011,[2020/05/14検索],
Attorney, Agent or Firm:
Shimizu Hatsushi
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto
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