Title:
ENDOGLYCOCERAMIDASE GENE
Document Type and Number:
Japanese Patent JP3527021
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject new gene containing a DNA coding a polypeptide having endoglycoceramidase activator or functionally the same activity, useful for industrially producing an enzyme for hydrolyzing a sphingoglycolipid.
SOLUTION: This new endoglycoceramidase gene has a DNA coding a polypeptide having an amino acid sequence of formula I or formula II or a part of it and endoglycoceramidase activity or functionally the same activity and is useful for industrially producing a gene which hydrolyzes a glycoside bond between a sugar chain of sphingoglycoside and a ceramide to liberate the sugar chain of a complete form and the ceramide by enzyme gene manipulation technology. The gene is obtained by separating a genome DNA from Rhodococcus sp. M-777, a bacterium capable of producing endoglycoceramidase II, preparing a DNA library by using the genome DNA, screening the library by using a probe and recovering the DNA from a positive clone.
Inventors:
Izu, Hiroyuki
Kurome, Yoko
Izumi, Yoshinari
Sano, Mutsumi
Katou, Ikunoshin
Ito, Makoto
Kurome, Yoko
Izumi, Yoshinari
Sano, Mutsumi
Katou, Ikunoshin
Ito, Makoto
Application Number:
JP18821996A
Publication Date:
May 17, 2004
Filing Date:
June 27, 1996
Export Citation:
Assignee:
TAKARA SHUZO CO LTD
International Classes:
C12N15/09; C07H21/04; C12N1/21; C12N9/24; C12N15/00; C12R1/01; C12R1/19; (IPC1-7): C12N15/09; C12N1/21; C12N9/24
Other References:
Ｂｉｏｃｈｅｍｉｓｔｒｙ， Ｖｏｌ．３１，Ｎｏ．３７，ｐｐ．９０００−９００７（１９９２）
Ｅｕｒ．Ｊ．Ｂｉｏｃｈｅｍ．， Ｖｏｌ．１８１，Ｎｏ．３，ｐｐ．５６３−５７０（１９８９）
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ，Ｖｏｌ．２６４，Ｎｏ．１６，ｐｐ．９５１０−９５１９（１９８９）
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ，Ｖｏｌ．２６９，Ｎｏ．２３，ｐｐ．１６２０３−１６２１１（Ｊｕｎｅ １０， １９９４）
Ｅｕｒ．Ｊ．Ｂｉｏｃｈｅｍ．， Ｖｏｌ．１８１，Ｎｏ．３，ｐｐ．５６３−５７０（１９８９）
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ，Ｖｏｌ．２６４，Ｎｏ．１６，ｐｐ．９５１０−９５１９（１９８９）
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ，Ｖｏｌ．２６９，Ｎｏ．２３，ｐｐ．１６２０３−１６２１１（Ｊｕｎｅ １０， １９９４）
Attorney, Agent or Firm:
細田 芳徳
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