Title:
挿入変異体ライブラリーを血清でスクリーニングすることによる、抗原的に重要な髄膜炎菌抗原の同定
Document Type and Number:
Japanese Patent JP2007517505
Kind Code:
A
Abstract:
A method for identifying a polypeptide of a microorganism which polypeptide is associated with an immune response in an animal which has been subjected to the microorganism, the method comprising the steps of (1) providing a plurality of different mutants of the microorganism; (2) contacting the plurality of mutant microorganisms with antibodies from an animal which has raised an immune response to the microorganism or a part thereof, under conditions whereby if the antibodies bind to the mutant microorganism the mutant microorganism is killed; (3) selecting surviving mutant microorganisms from step (2); (4) identifying the gene containing the mutation in any surviving mutant microorganism; and (5) identifying the polypeptide encoded by the gene. The polypeptide identified or a variant or fragment thereof or a fusion of these is useful in a vaccine. The polypeptide may be a polypeptide comprising the amino acid sequence selected from any one of SEQ ID Nos 2, 4, 6, 8, 10, 12, 14, 16, 18, 25 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56; or a fragment or variant thereof or a fusion of such a fragment or variant, and is useful in a vaccine against Neisseria meningitidis.
Inventors:
Christoph Marcel Tongue
Yang Wen Li
Yang Wen Li
Application Number:
JP2006546330A
Publication Date:
July 05, 2007
Filing Date:
December 23, 2004
Export Citation:
Assignee:
IMPERIAL INNOVATIONS LIMITED
International Classes:
C12N15/09; A61K39/02; A61K39/095; A61P31/04; C07K14/22; C07K19/00; C12N1/21; C12P21/02; C12Q1/02; C12Q1/04; C12Q1/68; A61K39/00
Attorney, Agent or Firm:
Masatake Shiga
Takashi Watanabe
Yasuhiko Murayama
Shinya Mitsuhiro
Takashi Watanabe
Yasuhiko Murayama
Shinya Mitsuhiro