Title:
組換えDNA由来のtPA又はK2S分子の大量生産方法
Document Type and Number:
Japanese Patent JP2004520018
Kind Code:
A
Abstract:
The invention belongs to the field of thrombolysis and of tissue plasminogen activator (tPA) derivative production in prokaryotic cells. The invention relates to methods for the production of a recombinant DNA-derived tPA, a variant thereof or a (Kringle 2 Serine) K2S molecule or a variant thereof in prokaryotic cells, wherein said tPA or K2S or variant is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for said tPA or K2S or variant operably linked to the DNA coding for the signal peptide OmpA. The invention further relates to specific K2S derivatives obtainable by said method. The invention further relates to said DNA molecules and the use of said DNA molecules in said methods.
Inventors:
Werner Rolf-Günter
Getz Friedrich
Tayapi Watana Chatchai
Manos Roy Gila Jay
Manos Roy Alanya
Getz Friedrich
Tayapi Watana Chatchai
Manos Roy Gila Jay
Manos Roy Alanya
Application Number:
JP2002543647A
Publication Date:
July 08, 2004
Filing Date:
November 07, 2001
Export Citation:
Assignee:
Boehringer Ingelheim International GmbH
International Classes:
A61K38/46; A61K38/48; A61P9/00; A61P9/10; A61P11/00; C07K19/00; C12N1/21; C12N9/64; C12N9/72; C12N15/09; C12N15/58; C12P21/02; (IPC1-7): C12N15/09; A61P9/00; A61P9/10; A61P11/00; C07K19/00; C12N1/21; C12N9/64
Domestic Patent References:
| JP2004513648A | 2004-05-13 |
Attorney, Agent or Firm:
Minoru Nakamura
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda
Fumiaki Otsuka
Sadao Kumakura
Shishido Kaichi
Toshio Imajo
Nobuo Ogawa
Village shrine Atsuo
Takaki Nishijima
Atsushi Hakoda