Title:
MEASUREMENT OF REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE(NADH) OR REDUCED NICOTINAMIDE DINUCLEOTIDE PHOSPHATE(NADPH)
Document Type and Number:
Japanese Patent JP3875366
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for measuring reduced nicotinamide adenine dinucleotide(NADH) or reduced nicotinamide dinucleotide phosphate(NADPH), enabling to rapidly and simply measure the NADH or the NADPH in high sensitivity.
SOLUTION: This method for measuring NADH or NADPH comprises electrochemically measuring the concentration of the NADH or the NADPH with two electrodes consisting of a working electrode and a counter electrode. Therein, the characteristics comprise applying a constant electric potential to the working electrode within a potential range not causing the electrolysis of the NADH or the NADPH, subsequently applying a pulse electric potential to the working electrode within a potential range causing the electrolysis of the NADH or the NADPH, and subsequently measuring an electric current flowing between the working electrode and the counter electrode at 10-1000 msec after the application of the pulse potential. The method enables to measure the NADH or the NADPH in high sensitivity having a detection limit concentration of ≤10 μmmol/L.
Inventors:
Mitsugu Senda
Application Number:
JP23834097A
Publication Date:
January 31, 2007
Filing Date:
September 03, 1997
Export Citation:
Assignee:
Arkray Co., Ltd.
International Classes:
C12Q1/26; (IPC1-7): C12Q1/26
Attorney, Agent or Firm:
Hiroyuki Ikeuchi
Kimihiro Sato
Naomi Matsumoto
Kimihiro Sato
Naomi Matsumoto
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