PURPOSE: To provide the subject substance useful for decoloring mold stains in houses and the stains and freckles of skins, etc., by culturing HAM-1 strain belonging to the molds to store the produced substance having an ability for decomposing the melamine pigment in the cells of the HAM-1 stain, and subsequently collecting the stored substance.
CONSTITUTION: This melamine pigment-decomposing substance useful for decoloring mold stains adhered to the walls, tiles, etc., of houses, stains or freckles produced on skins, etc., is obtained by inoculating a medium with HAM-1 strain (FERM P-14064 deposited in Biotechnology Industrial Technology Institute in Agency of Industrial Science and Technology), culturing the strain under conditions comprising a temperature of 30°C and the stirring rotation of 125rpm, adding indole-3-acetic acid to the culture solution, and further culturing the strain in the added culture solution for 50hrs to produce and store the substance having the activity for decomposing the melamine pigment, centrifugally recovering the cells from the culture solution, grinding the cells with a homogenizer under cooling with ice, centrifuging the ground cells, collecting the supernatant, adding ammonium sulfate to the supernatant under cooling with ice, centrifuging the salted-out precipitates, and subsequently purifying the recovered precipitates.
| JP5625199 | Pentose sugar fermented cells |
| WO/2009/110796 | PRODUCTION OF ITACONIC ACID |
| JPH1080291 | NEW COMPOUND WF15483, PRODUCTION AND USE THEREOF |
UEMATSU YASUSHI
FURUYA MASAHIRO
NAKAGAWA MICHIYA
