Title:
発現微量タンパク質/ペプチドの検出・分離・同定法
Document Type and Number:
Japanese Patent JP4679368
Kind Code:
B2
Abstract:
A method of detection, separation and identification for expressed trace protein/peptide; and a system therefor. There is provided a method of detecting, separating and identifying a minute amount of expressed protein and/or peptide, characterized in that a fluorescent derivative of protein and/or peptide contained in a test subject sample having been labeled with a fluorescence reagent is applied to HPLC; a fluorescent fraction is collected and subjected to enzymatic hydrolysis; mass-spectrometry of the resultant fluorescence-labeled fragments and non-fluorescence-labeled fragments is carried out; and the thus obtained ion molecular weight information on each of the fragments is collated with an available protein and/or peptide fragment database to thereby accomplish a structural analysis. Further, there is provided an identification system therefor.
Inventors:
Kazuhiro Imai
Application Number:
JP2005516223A
Publication Date:
April 27, 2011
Filing Date:
December 13, 2004
Export Citation:
Assignee:
Kazuhiro Imai
International Classes:
G01N27/62; G01N21/64; G01N21/77; G01N21/78; G01N30/72; G01N30/74; G01N30/88; G01N33/483; G01N33/533; G01N33/68; G01N27/447; G01N30/02; B01D
Other References:
鳥海 他1名,蛍光標識/LC-MS/MS/データベース検索による新規プロテオーム解析法,日本薬学会第123会年会要旨集,2003年 3月,123,第45頁28[P1]II-184
Chifuyu Toriumi and Kazuhiro Imai,An Identification Method for Altered Proteins in Tissues Utilizing Fluorescence Derivatization, Liquid Chromatography, Tandem Mass Spectrometry, and a Database-Searching Algorithm,Anal. Chem.,2003年 6月20日,75 (15),3725-3730
増田 他3名,ペプチド・タンパク質解析を指向したLC-MS/MS用高感度新規蛍光誘導体化試薬の開発,日本分析化学会第52会年会講演要旨集,2003年 9月,第49頁 3B11
Thomas Faller et al.,A novel acridone derivative for the fluorescence tagging and mass spectrometric sequencing of peptides,Chem. Commun.,1997年,1529-1530
Chifuyu Toriumi and Kazuhiro Imai,An Identification Method for Altered Proteins in Tissues Utilizing Fluorescence Derivatization, Liquid Chromatography, Tandem Mass Spectrometry, and a Database-Searching Algorithm,Anal. Chem.,2003年 6月20日,75 (15),3725-3730
増田 他3名,ペプチド・タンパク質解析を指向したLC-MS/MS用高感度新規蛍光誘導体化試薬の開発,日本分析化学会第52会年会講演要旨集,2003年 9月,第49頁 3B11
Thomas Faller et al.,A novel acridone derivative for the fluorescence tagging and mass spectrometric sequencing of peptides,Chem. Commun.,1997年,1529-1530
Attorney, Agent or Firm:
Masahiko Sudo