Title:
遺伝子送達の増強方法
Document Type and Number:
Japanese Patent JP7080184
Kind Code:
B2
Abstract:
The present invention provides improved methods for gene delivery to, or genetic modification of target cells, wherein the gene delivery or other genetic modification of the target cells is performed in the presence of endothelial cells, or after co-culture of the target cells with endothelial cells, or wherein co-culture of the target cells with endothelial cells is employed immediately alter gene delivery in order to “rescue” cells that may have been damaged during the gene delivery process. In some embodiments gene delivery is performed by transfection. In some embodiments gene delivery is performed by transduction, in some embodiments the endothelial cells are organ-specific endothelial cells. In some embodiments the endothelial cells are E40RF1-expressing endothelial cells (E40RF1+ ECs). In some embodiments the target cells are stem cells, such as hematopoietic stem cells.
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Inventors:
Paul William Finnegan
Claude Jeffrey Davis
Michael Daniel Ginsberg
Daniel Joseph Nolan
Claude Jeffrey Davis
Michael Daniel Ginsberg
Daniel Joseph Nolan
Application Number:
JP2018554116A
Publication Date:
June 03, 2022
Filing Date:
April 17, 2017
Export Citation:
Assignee:
Angiocrine Bioscience, Inc.
International Classes:
C12N5/10; C12N5/077; C12N5/0789; C12N15/09; C12N15/85; C12N15/86; C12N15/861; C12N15/864; C12N15/867; C12N15/869; C12N15/88; C12N15/89
Foreign References:
| US20070077652 | ||||
| WO2008089448A2 |
Other References:
Zhang, C. C. et al.,Hypoxia and metabolic properties of hematopoietic stem cells,Antioxid. Redox Signal,2014年,Vol. 20(12), pp. 1891-1901
Seandel, M. et al.,Generation of a functional and durable vascular niche by the adenoviral E4ORF1 gene,Proc. Natl. Acad. Sci. USA.,2008年,Vol. 105(49), pp. 19288-19293
Gori, J. L. et al.,In vivo selection and long-term engraftment of hematopoietic stem cells generated via vascular niche induction of nonhuman primate induced pluripotent stem cells,Blood,2013年,Vol. 122(21), Abstract Number: 466
Seandel, M. et al.,Generation of a functional and durable vascular niche by the adenoviral E4ORF1 gene,Proc. Natl. Acad. Sci. USA.,2008年,Vol. 105(49), pp. 19288-19293
Gori, J. L. et al.,In vivo selection and long-term engraftment of hematopoietic stem cells generated via vascular niche induction of nonhuman primate induced pluripotent stem cells,Blood,2013年,Vol. 122(21), Abstract Number: 466
Attorney, Agent or Firm:
Yamato Kento
Michiko Matsutani
Michiko Matsutani