To develop a method for producing sustained-release particulates of lactoferrin which enables expression of physiological activity by oral ingestion.
Lactoferrin is dissolved in an acid polymer, for example, an aqueous sodium alginate solution, and the resulting solution is poured into liquid paraffin containing sorbitan sesquioleate, followed by drying to prepare particulates comprising lactoferrin and sodium alginate. Then, a calcium chloride solution is added to the resulting particulates to convert it into particulates of lactoferrin and calcium alginate. A basic polymer, for example, an aqueous chitosan solution, is added to the particulates comprising lactoferrin and calcium alginate to perform a polymer blend to form a polymer alloy of lactoferrin, calcium alginate, and chitosan. The polymer alloy has a property of gradually releasing lactoferrin from several hours to one day when it is immersed in water.
KOYAMA KENICHI
MACHIDA RYOJI
| WO2006016595A1 | 2006-02-16 |
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