PURPOSE: To obtain the novel antibody specifically reacting with only one of human pepsinogens I and II in an immune reaction system, not reacting with the other, capable of specifically and accurately determining the human pepsinogen I or II, and useful for diagnosing various diseases, etc.
CONSTITUTION: A novel monoclonal antibody not reacting with human pepsinogen II (hPGII) and specifically reacting human pepsinogen I (hPGI) in an immune reaction system, and a novel monoclonal antibody not reacting with the hPGI and specifically reacting with the hPGII, or a fragment containing their hypervariable parts on the structures. The monoclonal antibodies are obtained by administering the hPGI or II together with an adjuvant into a BALB/c mouse for immunizing the mouse, collecting the splenic cells of the mouse after the final immunization, fusing the collected splenic cells with myeloma cells, selectively culturing the fused cells in a HAT medium, screening the obtained hybridoma, cloning the screened hybridoma, and subsequently culturing the obtained clone. The antibodies can be used for the accurate determination of the hPGI, II.
TERANO YOSHITAKE
SAMORI TOMOHIRO
KONISHI NOBORU
HIASA YOSHIO
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