Title:
複数パラメータースクリーニングおよび複数機能性小分子産生細胞への進化方法
Document Type and Number:
Japanese Patent JP4472345
Kind Code:
B2
Abstract:
The present invention relates to the field of selecting cells producing compounds that fulfil at least 2 predetermined functionalities. The invention primarily focuses on methods for screening of cells for two or more functionalities related to small molecules generated by host cells with novel gene assemblies. The methods in one aspect of the invention are combined with methods for evolution of cells to acquire at least two predetermined functionalities related to small molecules. Furthermore, the invention relates to methods for generation of lead compounds that are selected for a primary function and at least one further secondary function. The methods according to the invention may in particular be used in high troughput screening.
Inventors:
Neil Goldsmith
Seiren Wes Nielsen
Alexandra Emi Pe Santana Sorensen
Kurt Aime Fries Nielsen
Seiren Wes Nielsen
Alexandra Emi Pe Santana Sorensen
Kurt Aime Fries Nielsen
Application Number:
JP2003562286A
Publication Date:
June 02, 2010
Filing Date:
January 24, 2003
Export Citation:
Assignee:
Evolva Ltd.
International Classes:
C12N15/09; C12N5/10; C12N15/10; C12Q1/02; C12Q1/18; C12Q1/68; G01N33/50
Foreign References:
| WO1996034112A1 | ||||
| WO1998058085A1 |
Other References:
Genome research. 1996, Vol.6, No.9, p.791-806
Cytometry. 1999, Vol.37, No.1, p.51-59
Nature biotechnology. 1996, Vol.14, No.7, p.884-887
Cytometry. 1999, Vol.37, No.1, p.51-59
Nature biotechnology. 1996, Vol.14, No.7, p.884-887
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Michiko Matsutani
Mina Shiga
Sakurai Yoko
Satoshi Hashimoto
Minato Saito
Hiroshi Yamazaki
Michiko Matsutani
Mina Shiga
Sakurai Yoko
Satoshi Hashimoto
Minato Saito