Title:
光応答性細胞固定化剤
Document Type and Number:
Japanese Patent JP7236126
Kind Code:
B2
Abstract:
[Problem] The present invention addresses the problem of developing a photoresponsive cell fixing agent that can be used repeatedly and continuously, and can selectively and reversibly control the fixing and releasing of cells by photostimulation, regardless of the targeted cell type. [Solution] A photoresponsive cell fixing agent for fixing prescribed target cells on a substrate, said photoresponsive cell fixing agent being characterized by having a hydrophobic chain that can interact with the target cells, a hydrophilic chain that can be arranged in a monomolecular film shape on a surface of the substrate, and a branched linker chain that links the hydrophobic chain and the hydrophilic chain, wherein the photoresponsive cell fixing agent has, on a side chain of the branched linker chain, a photoisomerization site that is isomerized by light irradiation and can change from hydrophobic to hydrophilic.
Inventors:
Tetsushi Yamaguchi
Mori Izumida
Akamitsu Okamoto
Mori Izumida
Akamitsu Okamoto
Application Number:
JP2018105068A
Publication Date:
March 09, 2023
Filing Date:
May 31, 2018
Export Citation:
Assignee:
National University Corporation The University of Tokyo
International Classes:
C12N5/00; C07K5/033
Foreign References:
| WO2016158327A1 |
Other References:
HE, Di et al.,A photoresponsive soft interface reversibly controls wettability and cell adhesion by conformational changes in a spiropyran-conjugated amphiphilic block copolymer,Acta Biomaterialia,Vol.51,2017年,pp.101-111,特に要約,図1,図3,図7
TADA, Yuichi et al.,Development of a photoresponsive cell culture surface: regional enhancement of living-cell adhesion Induced by Local Light Irradiation,Journal of Applied Polymer Science,Vol.100,No.1,2006年,pp.495-499,特に図1
BRIEKE,C. et al.,Light-Controlled Tools,Angew. Chem. Int. Ed.,2012年,Vol.51,No.34,pp.8446-8476,特に図2
TADA, Yuichi et al.,Development of a photoresponsive cell culture surface: regional enhancement of living-cell adhesion Induced by Local Light Irradiation,Journal of Applied Polymer Science,Vol.100,No.1,2006年,pp.495-499,特に図1
BRIEKE,C. et al.,Light-Controlled Tools,Angew. Chem. Int. Ed.,2012年,Vol.51,No.34,pp.8446-8476,特に図2
Attorney, Agent or Firm:
Makoto Ono
Kanayama Sage
Yoshiyuki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Takahiro Aoki
Yoshie Sakurada
Yosuke Kawasaki
Takuya Gomibuchi
Toshikazu Imato
Youichi Iino
Yusuke Ichikawa
Masahiro Moriyama
Yoshikazu Iwase
Yasufumi Shiroyama
Kanayama Sage
Yoshiyuki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Takahiro Aoki
Yoshie Sakurada
Yosuke Kawasaki
Takuya Gomibuchi
Toshikazu Imato
Youichi Iino
Yusuke Ichikawa
Masahiro Moriyama
Yoshikazu Iwase
Yasufumi Shiroyama