Title:
POLYNUCLEOTIDE PRODUCING DEVICE
Document Type and Number:
Japanese Patent JPS57140799
Kind Code:
A
Abstract:
n apparatus for the stepwise synthesis of polynucleotides in which the nucleotide chains are extended in stepwise fashion from a solid polymer support to which the first unit is linked comprises a reaction column containing the polymer supported product and acting as the reaction vessel, and a series of reagent bottles all connected to the reaction column by means of a fluid flow conduit to which the vessels make connection via two-way valves arranged in series. The farthest upstream vessel of the series contains reaction solvent, used for washing purposes and the like. The most downstream of the reaction vessels contain nucleotide reagents. At least the valves associated with vessels containing nucleotide reagents have two separate and discrete fluid flow passageways, the first of which is used exclusively for flow of reagent from its associated vessel into the fluid flow conduit, and the second of which is used exclusively for flow of solvent or reagents from an upstream vessel therethrough, thereby eliminating the possibilities of cross-contamination as a result of nucleotide reagent residuals left in the dead space of the valve.
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Inventors:
ROBAATO BENDAA
PIITAA DEBUITSUDO DATSUKU
PIITAA DEBUITSUDO DATSUKU
Application Number:
JP736882A
Publication Date:
August 31, 1982
Filing Date:
January 19, 1982
Export Citation:
Assignee:
ENSU BAIO ROJIKARUZU INC
International Classes:
C12N15/09; B01J19/00; C07H21/00; C07H21/04; C40B40/06; C40B60/14; (IPC1-7): C07H21/02; C07H21/04