PURPOSE: To make easy and stable preparation of a bone marrow specimen of a single layer possible which makes it easy to discover a micronucleus and in which the dispersion of corpuscles is uniform, by adding a diluted solution to red corpuscles collected from a bone marrow left after nuclear corpuscles and blood platelets are removed and by smearing centrifugally a liquid thus obtained.
CONSTITUTION: A bone marrow 4 of a rat (or a mouse) is made to flow into a column 3 prepared by laying a filter paper piece 2 on the bottom of a syringe 1 and by packing a mixture of microcrystalline cellulose and α-cellulose thereon, nuclear corpuscles and blood platelets are made adsorbed by the column 3 and removed and a liquid flowing out is collected in a test tube 6. Next, unnecessary components in the liquid 6 are centrifuged from red corpuscles, a bovine fetus serum is added as a diluted solution to the red corpuscles collected, so that cell components occupy about 70%, and thereby a bone marrow liquid is prepared. By this method, about 99% of the nuclear corpuscles and about 90% of the blood platelets are removed and the bone marrow liquid almost all of which is constituted of the red corpuscles is obtained. By smearing this liquid centrifugally on slide glass, accordingly, a bone marrow specimen of a single layer in which only the red corpuscles necessary for a micronucleus test exist and the dispersion of the corpuscles is uniform can always be prepared stably.
| JP2005507474 | Exhaust line for internal combustion engine |
| JPH0829322 | MICROSCOPY AND HOLDER FOR MICROSCOPE |
KATO YOSHIHIKO
HAYATA ISAMU
YAMAMOTO MIKIO
OMURON RAIFU SCI KENKYUSHO KK