PURPOSE: To provide a lymphotoxin-immobilized carrier for therapies, etc., capable of selectively adsorbing lymphotoxin receptor-bearing cells thereto, thus capable of separating and eliminating leukocytes from the blood for transfusion or the blood of patients with rheumatism or autoimmune diseases.
CONSTITUTION: A lymphotoxin solution produced from Chinese hamster ovary cells by stimulating lymphocytes with a phorbol ester or mitogen, etc., or stimulating established lymphocyte-derived cells with e.g. mitogen, or by using a recombinant gene prepared with a cloned lymphotoxin CDNA, is incorporated with a 0.1M glycine buffer solution (pH 8.2) containing 0.15M NaCl, and the resultant solution is added to the wells of a carrier such as a plastic 96-well plate at 100μl per well and then allowed to stand overnight at 4°C followed by addition of a 1% bovine serum albumin solution to effect blocking, thus obtaining the objective lymphotoxin-immobilized carrier capable of specifically binding lymphotoxin receptor-bearing cells thereto.
SUMIYA TORU