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Title:
PROTEIN-IMMOBILIZED CARRIER AND SEPARATION OF CELL USING THE SAME
Document Type and Number:
Japanese Patent JPH0632800
Kind Code:
A
Abstract:

PURPOSE: To provide a lymphotoxin-immobilized carrier for therapies, etc., capable of selectively adsorbing lymphotoxin receptor-bearing cells thereto, thus capable of separating and eliminating leukocytes from the blood for transfusion or the blood of patients with rheumatism or autoimmune diseases.

CONSTITUTION: A lymphotoxin solution produced from Chinese hamster ovary cells by stimulating lymphocytes with a phorbol ester or mitogen, etc., or stimulating established lymphocyte-derived cells with e.g. mitogen, or by using a recombinant gene prepared with a cloned lymphotoxin CDNA, is incorporated with a 0.1M glycine buffer solution (pH 8.2) containing 0.15M NaCl, and the resultant solution is added to the wells of a carrier such as a plastic 96-well plate at 100μl per well and then allowed to stand overnight at 4°C followed by addition of a 1% bovine serum albumin solution to effect blocking, thus obtaining the objective lymphotoxin-immobilized carrier capable of specifically binding lymphotoxin receptor-bearing cells thereto.


Inventors:
OOHARA TAKAAKI
SUMIYA TORU
Application Number:
JP18783992A
Publication Date:
February 08, 1994
Filing Date:
July 15, 1992
Export Citation:
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Assignee:
KANEGAFUCHI CHEMICAL IND
International Classes:
C07K17/04; C07K14/52; C07K14/525; C12N5/07; C12N5/0786; (IPC1-7): C07K17/04; C12N5/08
Attorney, Agent or Firm:
Sota Asahina (1 person outside)