PURPOSE: To purify the titled compound, by bringing a solution containing interleukin 2 into contact with porous glass beads, adsorbing the interleukin 2 in the glass beads, and eliminating and eluting the interleukin 2 from the porous glass beads.
CONSTITUTION: A solution containing interleukin 2 (IL-2), e.g. a human peripheral blood, splenic cell or human T leukocytic cell, at 5W10pH is brought into contact with porous glass beads (CPG), and adsorbed therein. The ionic strength of the IL-2 solution in the adsorption is preferably ≤0.5M based on the NaCl concentration. The adsorbed interleukin 2 adsorbed in the CPG is then eluted with an eluent, e.g. a buffer solution containing 1W99% ethylene glycol or glycerol, and the resultant IL-2 solution is then separated by the concentration with a membrane, salting out, etc. and used for an antitumor drug or diagnostic agent, etc.
EFFECT: An economic method having a high recovery ratio, simplified desalting steps and high efficiency.
TSUJI HISASHI
IWASHITA YUUJI
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