PURPOSE: To obtain a reagent for a clotting reaction between a reverse passive antibody and a red blood cell having high specificity to the ovary cancer and blasenmole by sensitizing the specific antihuman BMAP antibody obtd. by immunizing the refined basement membrane protein (abbreviated as BMAP) obtd. from the human placenta to an animal with the red blood cell of another animal.
CONSTITUTION: The BMAP which is recovered from the human placenta and has about 10,000mol.wt., 3.5pH at the isoelectric point, and the easy mobility by electrophoresis in the β- globulin region and contains 10% hexose is immunized to a horse, by which the antihuman BMAP antibody is obtd. Such anitbody which is sensitized with the red blood cell of a sheep is freeze-dried and is suspended in a phosphoric acid buffer soln., to which sodium azide is added and the reagent that can be preserved is obtd. A kit is constituted of such reagent, a standard human plasma positive control, a standard human plasma negative control and a buffer soln. for measurement. A specified amt. of the reagent is added to the double-double diluted system of a liquid to be examined and the resulted precipitate is compared with the clotting of the positive control and the settling image (small and distinct) of the negative control and the max. dilution time at which the positive clotting is admitted is determined as the quantity of the human BMAP. The quick and exact diagnosis of the ovary cancer and blasenmole is thus made possible.
FUKUSHIMA TSUNEKAZU
FUNAKOSHI SATORU
SUYAMA TADAKAZU
Next Patent: AUTOMATIC ANALYZING APPARATUS