Title:
SERUM-FREE CULTURE MEDIUM, METHOD FOR CULTURING ANIMAL CELL AND PRODUCTION OF FOREIGN PROTEIN
Document Type and Number:
Japanese Patent JPH04234982
Kind Code:
A
Abstract:
PURPOSE: To provide a serum-free culture medium, capable of exhibiting cell proliferating properties and foreign protein productivity equal to those in using a serum culture medium and suitable for growing animal cells.
CONSTITUTION: A serum-free culture medium (GCM001 culture medium) is obtained by using RPMI1640 culture medium as a basal culture medium and including insulin, BP (peptone derived from beef), transferrin, HSA (human blood serum albumin), hypoxanthine, thymidine and selenium therein.
Inventors:
AMATSUJI YASUO
NAKAJIMA MASAHIDE
TANABE TOSHIZUMI
KAWABE HARUHIDE
NAKAJIMA MASAHIDE
TANABE TOSHIZUMI
KAWABE HARUHIDE
Application Number:
JP41705990A
Publication Date:
August 24, 1992
Filing Date:
December 27, 1990
Export Citation:
Assignee:
GREEN CROSS CORP
International Classes:
C12N5/10; C12N5/07; C12N5/071; C12N9/72; C12P21/00; C12R1/91; (IPC1-7): C12N5/06; C12N5/10; C12N9/72; C12P21/00
Domestic Patent References:
| JPH025859A | 1990-01-10 | |||
| JPS6062981A | 1985-04-11 |
Attorney, Agent or Firm:
Takashima Hajime