Title:
【発明の名称】非病原性トランスフェクションベクター
Document Type and Number:
Japanese Patent JP2002533088
Kind Code:
A
Abstract:
A non-viral transfection vector which is to be delivered into the nucleus of a cell comprises a DNA molecule that is equipped with 1 to 15 NLS conjugates. Each conjugate comprises a nuclear localization signal (NLS) covalently linked to an oligonucleotide. The NLS conjugate may be covalently linked to one or both termini of a linear DNA molecule, associated with a plasmid DNA molecule by forming a triple helix, or inserted in a plasmid DNA molecule by strand invasion. The transfection vector is useful for gene therapy applications.
Inventors:
Bale Jean Paul
Bergous-Valladiere Pascal
Zanta Maria-Antonietta
Bergous-Valladiere Pascal
Zanta Maria-Antonietta
Application Number:
JP2000589713A
Publication Date:
October 08, 2002
Filing Date:
December 22, 1999
Export Citation:
Assignee:
Universite Louis Pasteur de Strasbourg
International Classes:
A61K35/76; A61K38/00; A61K47/48; A61K48/00; C12N15/79; C12N15/85; C12N15/09; (IPC1-7): C12N15/09; A61K38/00; A61K47/48; A61K48/00
Attorney, Agent or Firm:
Minoru Nakamura (9 outside)