PURPOSE: To raise hydrolysis ratio of fats and oils containing long-chain unsaturated fatty acid, and to obtain fatty acid having high neutralization value in high yield, by combining specific enzymes.
CONSTITUTION: Fats and oils containing ≥20C long-chain unsaturated fatty acid such as fish oil, whale oil, colza oil, mustard oil, etc. are hydrolyzed by using an enzyme for hydrolyzing fats and oils having selectivity for triglyceride position and an enzyme for hydrolyzing fats and oils having no selectivity for triglyceride position. Lipase obtained from Aspergillus niger, Rhizopus delemar, and pancreatin is preferably used as the enzyme of the former, and lipase obtained from Geotrichum candidum, and Candida cylindracea as the enzyme of the latter. A ratio of the hydrolyzing enzymes is 1:(1W30) based on active unit, and an amount of water is 30W500wt% based on fats and oils. Hydrolyzing ratio by this method is improved by about 20% compared with a method to use one enzyme.
SUZUKI KAZUAKI
NAKAI EIJI
| JPS576480A | 1982-01-13 |