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Title:
A cell viewing device, a cell observation method, and its program
Document Type and Number:
Japanese Patent JP6278519
Kind Code:
B2
Abstract:
A cell monitoring device includes an outline detecting section that detects edge pixels from a cell image in a captured image of cells arranged in a single layer and generates an edge image including the detected edge pixels; a pigmented region detecting section that detects pixels of a pigmented region of the cell image in the captured image, and generates a pigmented region image including the detected pixels of the pigmented region; and an image merging section that, in a merged image obtained by overlaying the edge image and the pigmented region image together, detects a cell image region and a background image region in the captured image based on the pixel intensity variance and thus detects the cell image region in the captured image.

Inventors:
Sadaichi Oya
Shigeyuki Kawano
Satoru Nogami
Shinsuke Onuki
Shuhei Daejeon
Koichi Watanabe
Application Number:
JP2014549862A
Publication Date:
February 21, 2018
Filing Date:
November 27, 2013
Export Citation:
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Assignee:
Japan Science and Technology Agency
International Classes:
C12M1/34; C12Q1/02; G01N21/27; G06T7/00; G06T7/12; G06T7/174
Domestic Patent References:
JP2012021904A
JP2009175334A
JP2010063403A
JP2007097584A
JP2011030494A
JP2004532410A
Foreign References:
US20090213214
WO2002067195A1
Other References:
Plant Cell Physiol., 2013, 54(11), pp.1917-1929 (Epub 2013 Sep 20)
藻類、2013年3月、61巻、1号、44頁、B17
PLoS One, 2011, 6(9), e24302, pp.1-7
Biotechnol. Bioeng., 2008, 99(4), pp.1034-1039
Biosens. Bioelectron., 2005, 21(5), pp.768-773
Cytometry, 1997, 28, pp.289-297
LI, F-M., "Cells segmentation using the hybrid of image morphology and edge detector algorithm and cell counting", Case Western Reserved University [online], 2007.12.20 [検索日 2017.6.22], インターネット
Attorney, Agent or Firm:
Masatake Shiga
Suzuki Mitsuyoshi
Makiko Otsuki